Trophoblast-like cells sorted from peripheral maternal blood using flow cytometry: A multiparametric study involving transmission electron microscopy and fetal dna amplification

Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities.     

Gold mining in Siberia and the Far East as a source of mercury contamination of the environment

ＩｎｔｒｏｄｕｃｔｉｏｎＳｉｂｅｒｉａａｎｄｔｈｅＦａｒＥａｓｔａｒｅｔｈｅｏｌｄｅｓｔａｎｄｔｈｅｒｉｃｈｅｓｔｇｏｌｄ ｍｉｎｉｎｇａｒｅａｓｏｆＲｕｓｓｉａ .Ｉｎｔｅｎｓｉｖｅｇｏｌｄ ｍｉｎｉｎｇｈｅｒｅｓｔａｒｔｅｄｉｎｔｈｅｂｅｇｉｎｎｉｎｇｏｆｔｈｅ 19ｔｈｃｅｎｔｕｒｙ(Ｖｙａｚｅｌｓｈｃｈｉｋｏｖ ,1963) .Ｆｏｒｔｈｅｍｏｓｔｐａｒｔｇｏｌｄ ｂｅａｒｉｎｇｏｒｅｓａｎｄｓａｎｄｓｗｅｒｅｐｒｏｃｅｓｓｅｄｗｉｔｈｔｈｅｕｓｅｏｆａｍａｌｇａｍａｔｉｏｎｍｅｔｈｏｄｆｏｒｇｏｌｄｅ…     

Attitudes to prenatal screening,diagnosis and research among pregnant women who accept or decline an alpha-fetoprotein test

The aim of the study was to describe the opinion of pregnant women who had accepted or declined an alpha-fetoprotein (AFP) test, not only on AFP screening in general, but also on whether every pregnant woman should be offered amniocentesis (AC)/chorionic villus sampling (CVS) and an ultrasound scan for fetal malformations. An additional aim was to describe pregnant women's attitudes concerning continued research in the prenatal field. The study was performed as a questionnaire study in two regions over a 1-year period from 1 October 1988 to 30 September 1989. Results are based on answers from 3331 women who had taken an AFP test and 336 women who had declined the offer of a test. A total of 79 per cent of the women thought that an AFP test, 70 per cent that an ultrasound scan for fetal malformations, and 26 per cent that AC or CVS should be offered to all pregnant women. Fifty-nine per cent of the women were positive towards continued research in the prenatal field. Women who had had an AFP test were generally much more positive towards screening and research than women who had declined, who were generally against. Women who had left school without a high school degree were on average more positive towards the screening issues than women who had this degree. In conclusion, the results obtained in this study strongly suggest that women's attitudes are very dependent on how the prenatal screening programme is already organized in their local area.     

Prenatal diagnosis of medium-chain acyl-CoA dehydrogenase (MCAD) deficiency in a family with a previous fatal case of sudden unexpected death in childhood

Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is a potentially fatal inherited disease with a carrier frequency of approximately 1:100 in most Caucasian populations. The disease is implicated in sudden unexpected death in childhood. A prevalent disease-causing point mutation (A985G) in the MCAD gene has been characterized, thus rendering diagnosis easy in the majority of cases. Since the clinical spectrum of MCAD deficiency ranges from death in the first days of life to an asymptomatic life, there are probably other genetic factors—in addition to MCAD mutations—involved in the expression of the disease. Thus, families who have experienced the death of a child from MCAD deficiency might have an increased risk of a seriously affected subsequent child. In such a family we have therefore performed a prenatal diagnosis on a chorionic villus sample by a highly specific and sensitive polymerase chain reaction (PCR) assay for the G985 mutation. The analysis was positive and resulted in abortion. We verified the diagnosis by direct analysis on blood spots and other tissue material from the aborted fetus and from family members.     

Review of information on pesticide residues in the Canadian environment

Summary Pesticide residues in soil, water, food, wildlife and other media have been analysed in several monitoring studies. The purpose was to establish the distribution of these residues across a defined area, ascertain their trend over a specific period and use the results to assess environmental compliance and safety. In the present review the availability of information on pesticide residues in various Canadian environmental components was sought. With the possible exception of localized pesticide contamination of some private water supply wells, ground and surface water, most analyses revealed concentrations below guideline limits. Only a few cases were reported of mishandling, misuse and poisoning from pesticides. Continuing surveillance programmes and in-depth and well-organized monitoring studies, with special focus on areas that are vulnerable to contamination, by both provincial and federal governments, are largely responsible for the encouraging results. This experience in the control of pesticide use and monitoring of residues in the environment should be of particular interest in developing countries.Dr A.Y. Sangodoyin is a senior lecturer in the Department of Agricultural Engineering at the University of Ibadan. He was recently involved in a study visit to the University of Alberta, Edmonton, Alberta, Canada T6G 2G7, where his co-author Dr D.W. Smith is a member of staff in the Department of Civil-Engineering.     

Enzyme-Retted Flax Fiber and Recycled Polyethylene Composites

Municipal solid wastes generated each year contain potentially useful and recyclable materials for composites. Simultaneously, interest is high for the use of natural fibers, such as flax (Linum usitatissimum L.), in composites thus providing cost and environmental benefits. To investigate the utility of these materials, composites containing flax fibers with recycled high density polyethylene (HDPE) were created and compared with similar products made with wood pulp, glass, and carbon fibers. Flax was either enzyme- or dew-retted to observe composite property differences between diverse levels of enzyme formulations and retting techniques. Coupling agents would strengthen binding between fibers and HDPE but in this study fibers were not modified in anyway to observe mechanical property differences between natural fiber composites. Composites with flax fibers from various retting methods, i.e., dew- vs. enzyme-retting, behaved differently; dew-retted fiber composites resulted in both lower strength and percent elongation. The lowest level of enzyme-retting and the most economical process produces composites that do not appear to differ from the highest level of enzyme-retting. Flax fibers improved the modulus of elasticity over wood pulp and HDPE alone and were less dense than glass or carbon fiber composites. Likely, differences in surface properties of the various flax fibers, while poorly defined and requiring further research, caused various interactions with the resin that influenced composite properties.     

Tillage erosion and its effect on soil properties and crop yield in Denmark

Tillage erosion had been identified as a major process of soil redistribution on sloping arable land. The objectives of our study were to investigate the extent of tillage erosion and its effect on soil quality and productivity under Danish conditions. Soil samples were collected to a 0.45-m depth on a regular grid from a 1.9-ha site and analyzed for 137Cs inventories, as a measure of soil redistribution, soil texture, soil organic carbon (SOC) contents, and phosphorus (P) contents. Grain yield was determined at the same sampling points. Substantial soil redistribution had occurred during the past decades, mainly due to tillage. Average tillage erosion rates of 2.7 kg m(-2) yr(-1) occurred on the shoulderslopes, while deposition amounted to 1.2 kg m(-2) yr(-1) on foot- and toeslopes. The pattern of soil redistribution could not be explained by water erosion. Soil organic carbon and P contents in soil profiles increased from the shoulder- toward the toeslopes. Tillage translocation rates were strongly correlated with SOC contents, A-horizon depth, and P contents. Thus, tillage erosion had led to truncated soils on shoulderslopes and deep, colluvial soils on the foot- and toeslopes, substantially affecting within-field variability of soil properties. We concluded that tillage erosion has important implications for SOC dynamics on hummocky land and increases the risk for nutrient losses by overland flow and leaching. Despite the occurrence of deep soils across the study area, evidence suggested that crop productivity was affected by tillage-induced soil redistribution. However, tillage erosion effects on crop yield were confounded by topography-yield relationships.