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排序方式: 共有115条查询结果,搜索用时 312 毫秒
1.
Dr Marianne Johansen Marian Knight Edward J. Maher Kim Smith Ian L. Sargent 《黑龙江环境通报》1995,15(10):921-931
Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method. 相似文献
2.
Hasselquist Eliza Maher Mancheva Irina Eckerberg Katarina Laudon Hjalmar 《Ambio》2020,49(7):1341-1351
Ambio - Improving water quality has become an important environmental issue, spurred in part by the Water Framework Directive. However, the relationship of policy change with forest water... 相似文献
3.
随着地下工程与城市管网的布设日益发展,在规划设计阶段往往需要考虑潜在发生的安全事故影响,确定安全距离。利用ANSYS/LS-DYNA有限元软件,结合天津滨海新区饱和软土特点,分析了可能发生的燃气管线爆炸所产生的冲击荷载对土中埋置盾构隧道的结构安全影响。运用有限元数值模拟,得到了隧道与管线的间距—隧道结构振速峰值的曲线关系;通过对标相关规范的振速限制,得到不同燃气压力下隧道—管线的安全避让距离关系。结果表明:随着燃气管道压强的增大,隧道—管线的安全避让距离呈现先增大后平稳的趋势。结论对于爆破与防护工程设计均具有重要的参考价值。 相似文献
4.
Inhalation of particulate pollutants below 10 μm in size (PM10) is associated with adverse health effects. Here we use magnetic remanence measurements of roadside tree leaves to examine levels of vehicle-derived PM around Lancaster, UK. Leaf saturation remanence (SIRM) values exhibit strong correlation with both the SIRM and particulate mass of co-located, pumped-air samples, indicating that these leaf magnetic values are an effective proxy for ambient PM10 concentrations. Biomagnetic monitoring using tree leaves can thus provide high spatial resolution data sets for assessment of particulate pollution levels at pedestrian-relevant heights. Leaf SIRM values not only increase with proximity to roads with higher traffic volumes, but are also ~100% higher at 0.3 m than at ~1.5–2 m height. Magnetic and SEM data indicate that the particle populations are dominated by spherical, iron-rich particles ~0.1–1 μm in diameter, with fewer larger, more angular, silica-rich particles. Comparison of the roadside leaf-calculated PM10 concentrations with PM10 concentrations predicted by a widely-used atmospheric dispersion model indicates some agreement between them. However, the model under-predicts PM10 concentrations at ‘urban hotspots’ such as major–minor road junctions and traffic lights. Conversely, the model over-predicts PM10 concentrations with distance from the road wherever one tree is screened by another, indicating the filtering/protective effect of roadside trees in leaf. 相似文献
5.
Biodegradability of alkylates as a sole carbon source in the presence of ethanol or BTEX 总被引:1,自引:0,他引:1
The biodegradability of alkylate compounds in serum bottles was investigated in the presence and absence of ethanol or benzene, toluene, ethylbenzene, and p-xylene (BTEX). The biomass was acclimated to three different alkylates, 2,3-dimethylpentane, 2,4-dimethylpentane and 2,2,4-trimethylpentane in porous pot reactors. The alkylates were completely mineralized in all three sets of experiments. They degraded more slowly in the presence of BTEX than in their absence because BTEX inhibited the microbial utilization of alkylates. However, in the presence of ethanol, their slower biodegradation was not related to inhibition by the ethanol. Throughout the experiments alkylates, ethanol, and BTEX concentrations did not change in the sterile controls. 相似文献
6.
7.
高黏度污泥螺带螺杆搅拌混合特性的数值模拟 总被引:1,自引:0,他引:1
污泥处理过程通常需要加入添加剂,而高黏度污泥流动性差,搅拌混合是一个难点。选用螺带螺杆搅拌器加添加剂搅拌高黏度污泥,用Solid Works软件几何建模后,将模型导入Gambit划分网格,结合Fluent软件选用多重参考系法(MRF)分别对双螺带螺杆搅拌器与单螺带螺杆搅拌器下的污泥与添加剂混合过程进行了模拟,对比了两种搅拌桨的混合时间、体积分数流场分布及混合效率,分析了不同加料区域的影响,并将搅拌功率模拟与试验结果进行了对比。结果表明:螺带螺杆搅拌器适用于高黏度污泥的搅拌且双螺带螺杆搅拌器混合时间更短,体积分数流场更快趋于稳定且混合效率更高;最佳加料区域为搅拌槽的中部。 相似文献
8.
Ray Maher Melanie Maher Clive A. McAlpine Samuel Mann Leonie Seabrook 《Sustainability Science》2018,13(5):1357-1373
Sustainability challenges rarely align with the conventional boundaries of our disciplines, institutions and means of communication. To address these challenges amid real-world complexity, we need to think holistically and collaborate across disciplines. In this paper, we synthesise three themes: (1) more integrated conceptual frameworks; (2) digital visual communication which provides fluid expression of complex ideas and perceptions; and (3) online networks which can empower sustainability initiatives and communicate them across social and institutional barriers at a global scale. Each of these tools can help to overcome persistent barriers to sustainability. When used together, they provide a strategic basis for the design of digital collaboration platforms for addressing sustainability challenges. Using design thinking, we developed a Synergy Map which identifies relationships among a number of barriers to sustainability and conceptual and digital tools which help to address them. The Map identifies the potential for synthesising these tools into effective digital artefacts. We provide several examples and identify characteristics of particular value for overcoming barriers to sustainability. Combining new theoretical developments in sustainability sciences with recent advances in communication and networking technologies offers substantial potential for advancing sustainability on multiple fronts. 相似文献
9.
Dr Abdal‐Majeed I. Daghistani BA MSc PhD MRTPI 《Journal of Environmental Planning and Management》1991,34(1):2-9
The towns and cities of Saudi Arabia have been subjected to sustained growth pressures since the transformation of the country's economy following the discovery of oil in the Eastern Province of the Kingdom. The rapid growth which has been experienced has created major problems for the central and local government agencies responsible for planning and managing urban growth. The experience of Jeddah, one of the major cities in Saudi Arabia, is described and the problems of planning and managing its growth are analysed. Weaknesses relating to the structure of the planning system are identified, as are major difficulties in relation to the quality and quantity of professional staff. Recent attempts to improve the operation of the planning system are described and evaluated. 相似文献
10.