Snow cover and vegetation greenness with leaf water content control the global land surface temperature

The land surface temperature (LST) and land use land cover (LULC) are the major components of climate- and environment-related studies. The objective of this study was to assess the relationship of LST with remotely sensed LULC-derived vegetation indices during 2018 at the global, latitudinal and continental scales. Moderate resolution imaging spectroradiometer (MODIS) Aqua daytime LST and eight LULC MODIS indices (NDVI, EVI, LAI, DSI, NDWI, albedo, NDSI, NDBI) were processed using Earth Engine Code Editor. The analysis was conducted using correlation coefficient and significance of the relationship of variables based on 2050 randomly selected points at the global scale. Based on the univariate and geographically weighted regression methods, the research confirmed that vegetation greenness (NDVI), leaf water content (NDWI) and snow cover (DSI) are the codominant drivers of decreasing LST at the global scale including Europe, Asia, South America and North America at the continental scale. Snow cover during winter and vegetation greenness in summer seasons control the global LST. Although albedo shows an inverse relationship, NDBI and NDSI displayed a positive relationship to LST at the global scale. In conclusion, temporal seasonal and inter-annual dynamics of LST in response to snow cover and vegetation properties (greenness, moisture) should be focused on understanding and regulating LST at varying scales.

     

Statistical Optimization of Polyhydroxybutyrate Production by <Emphasis Type="Italic">Bacillus Pumilus</Emphasis> H9 Using Cow Dung as a Cheap Carbon Source by Response Surface Methodology

A gram positive bacterium (designated strain H9) found to be a potential polyhydroxybutyrate (biodegradable polymer) producer was isolated from the soil samples of a stress prone environment (municipal waste areas). This bacterium was identified as Bacillus pumilus H9 from its morphological, physiological and 16S rRNA gene sequence analysis. A four-factor central composite rotary design was employed to optimize the medium and to find out the interactive effects of four variables, viz. concentrations of cow dung, sucrose, peptone and pH on PHB production. Using response surface methodology, a second-order polynomial equation was obtained by multiple regression analysis and a yield of 2.47 g/L of PHB dry weight was achieved from the optimized medium at pH 7. Here, we report cow dung as a cheap carbon source for the production of PHB. Further, phbA, phbB and phbC genes were amplified by polymerase chain reaction which confirms the bacterium to be able to produce polyhydroxybutyrate.     

Carbaryl stress induced cellular changes in Calothrix brevissima

Cyanobacterial biofertilizers are affected by paddy field pesticides as nontarget organism. Carbaryl is a carbamate pesticide and is commonly used against rice thrip pest in paddy fields. In the present work, cellular changes caused by exposure of the cyanobacterial biofertilizer namely Calothrix brevissima to carbaryl were studied with special reference to fatty acids, electrolyte leakage, sulfur metabolism, and osmolytes. To study the toxic effect of carbaryl, the test cyanobacterium was exposed to varying concentrations of pesticide (0, 10, 20, 30, and 40 mg L^?1) for biochemical analyses. At 40 mg L^?1 carbaryl, polyunsaturated fatty acids were reduced by 32 % and membrane leakage was increased by 27 % suggesting that free radical-mediated lipid peroxidation took place. The sulfur-containing metabolites namely cysteine, cystine, and methionine were increased by 79, 64, and 52 %, respectively. The enzymatic and nonenzymatic antioxidants namely glutathione S-transferase, glutathione reductase, reduced glutathione, and oxidized glutathione were increased to 56, 71, 72, and 60 %, respectively. Osmolytes that serve as stress enzyme protectors as well as nonenzymatic free radical scavenger were also increased, indicating their protective nature in context with carbaryl-induced stress. The respective increase in mannitol, trehalose, and glycogen were 158, 98, and 159 %. In C. brevissima, carbaryl-induced membrane leakage was counteracted by increasing enzymatic and nonenzymatic parameters that helped in scavenging free radicals.