Statistical analyses of regional surface water quality in southeastern Ontario

Historical records from Ontario's Provincial Water Quality Monitoring Network (PWQMN) for rivers and streams were analyzed to assess the feasibility of mapping regional water quality patterns in southeastern Ontario which spans two major geologic zones, the Precambrian Shield and the St. Lawrence Lowlands, thus serving as a paradigm for much of Ontario. Despite biases toward the populated Lowlands and associated pollution problems, general spatial trends are evident via the analysis of intervariable relations, individual parameter maps and multivariate analysis. Using a robust algorithm designed to identify outliers and abstract underlying bivariate relations, a linear regional hardness-alkalinity relation was derived, where most anomalies proved to be mine drainage impacted sites. Regionally, multivariate ordination reveals that central tendencies of common indicators of ionic strength and nutrient richness correlate positively and that site clusters broadly reflect the transition from oligotrophic Shield waters to eutrophic conditions of heavily agricultural Lowland streams. Results suggest that on the Shield more precisely delineated aquatic regions may be realized by applying GIS to integrate river and synoptic lake survey data. Further synthesis with bedrock and surficial geology, physiography, pedology and other temporally invariant spatial attributes should yield regional patterns of background quality from which locally attainable water objectives might be defined. Additional synoptic surveys of sedimentary Lowland streams may be required as most available sites relflect anthropogenic pollution.     

The influence of acidic mine and spoil drainage on water quality in the mid-Wales area

The many abandoned base metal mines of the mid-Wales ore field are sources of extensive pollution. Some of the mineralised veins contain large amounts of pyrite and marcasite and oxidative weathering of these produces sulphuric acid resulting in very acidic mine drainage waters. In addition, the spoil tips associated with these mines can contain abundant iron sulphides. Drainage waters from these sources have pH values as low as 2.6 and are heavily contaminated with metals such as Al, Zn, Cd and Ni.Two of the main rivers of the area, the Rheidol and Ystwyth, intercept heavily contaminated acidic drainage which has a marked effect on water quality. The Rheidol contains over 100 g L^–1 Zn for 16 km downstream of the acid water influx. This level is over three times the recommended EEC limit for Zn in salmonoid waters of low hardness.     

A national framework for monitoring and reporting on environmental sustainability in Canada

In 1991, a collaborative project to revise the terrestrial component of a national ecological framework was undertaken with a wide range of stakeholders. This spatial framework consists of multiple, nested levels of ecological generalization with linkages to existing federal and provincial scientific databases. The broadest level of generalization is the ecozone. Macroclimate, major vegetation types and subcontinental scale physiographic formations constitute the definitive components of these major ecosystems. Ecozones are subdivided into approximately 200 ecoregions which are based on properties like regional physiography, surficial geology, climate, vegetation, soil, water and fauna. The ecozone and ecoregion levels of the framework have been depicted on a national map coverage at 1:7 500 000 scale. Ecoregions have been subdivided into ecodistricts based primarily on landform, parent material, topography, soils, waterbodies and vegetation at a scale (1:2 000 000) useful for environmental resource management, monitoring and modelling activities. Nested within the ecodistricts are the polygons that make up the Soil Landscapes of Canada series of 1:1 000 000 scale soil maps. The framework is supported by an ARC-INFO GIS at Agriculture Canada. The data model allows linkage to associated databases on climate, land use and socio-economic attributes.     

Interlaboratory evaluation of endotoxin analyses in agricultural dusts--comparison of LAL assay and mass spectrometry

Endotoxin exposure is associated with wheeze and asthma morbidity, while early life exposure may reduce risk of allergy and asthma. Unfortunately, it is difficult to compare endotoxin results from different laboratories and environments. We undertook this study to determine if lipopolysaccharide (LPS) extraction efficiency could account for differences among laboratories. We generated and collected aerosols from chicken and swine barns, and corn processing. We randomly allocated side-by-side filter samples to five laboratories for Limulus assay of endotoxin. Lyophilized aliquots of filter extracts were analyzed for 3-hydroxy fatty acids (3-OHFAs) as a marker of LPS using gas chromatography-mass spectrometry. There were significant differences in endotoxin assay and GC-MS (LPS) results between laboratories for all dust types (p < 0.01). Patterns of differences between labs varied by dust type. Relationships between assay and GC/MS results also depended on dust type. The percentages of individual 3-OHFA chain lengths varied across labs (p < 0.0001) suggesting that each lab recovered a different fraction of the LPS available. The presence of large amounts of particle associated LPS and absence of a freezing thawing cycle were associated with lower correlations between LPS and bioactivity, consistent with an absence of Limulus response to cell-bound endotoxin. These data suggest that extraction methods affect endotoxin measurements. The LAL methods may be most suitable when comparing exposures within similar environments; GC-MS offers additional information helpful in optimizing sample treatment and extraction. GC-MS may be of use when comparing across heterogeneous environments and should be considered for inclusion in future studies of human health outcomes.     

Robust graphical methods for diagnosing trend in irregularly spaced water quality time series

Graphical methods can play an important role in the reliable assessment of trends in typically ill behaved river quality data series both as diagnostic tools and as visual corroborative evidence when assumptions required for formal statistical tests are not met. Robust, graphically-oriented trend diagnosis procedures are presented for data series characterized by nonnormal populations, uneven time spacing, nonmonotonic trend and other factors which can create serious problems for standard parametric time series methods. Cleveland's robust locally weighted regression (RLWR) developed for investigating nonlinearity in x-y scatterplots is adapted as a robust/resistant smoothing filter for the analysis of irregular time series comprising quantitative observations. Low powered RLWR trend lines reveal temporally local phenomena, e.g. abrupt jumps (often associated with point source impacts) and periodicities, while higher powered RLWR yields smooth lines characterizing medium and longer term trends. Simple variants of Tukey smoothing concepts are developed for series with censored observations. Applications to Ontario river quality series reveal that graphical evidence is frequently sufficient to obviate the need for formal trend testing. The methods are generally applicable to most time series.     

An investigation of methods for enriching trophoblast from maternal blood

Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method.     

Prenatal ultrasound diagnosis of Apert's syndrome

A mother affected with Apert's syndrome was diagnosed by ultrasound scan at 16–17 weeks to have a fetus similarly affected. The typical features of acrocephaly and symmetrical syndactyly were seen. This is probably the first time that this condition has been diagnosed at such a gestation by ultrasound scan. The patient decided to continue the pregnancy, and intrauterine death occurred at 34 weeks. The diagnosis was confirmed by pathological examination.     

A decision tree approach modelling functional group abundance in a pasture ecosystem

Decision tree, one of the data mining approaches, was used to model the relative abundance of five functional groups of plant species, namely high fertility response grasses (HFRG), low fertility tolerance grasses (LFTG), legume, moss and flatweeds in a New Zealand hill-pasture ecosystem using aboveground biomass. The model outputs were integrated with a geographic information system (GIS) to map and validate the predictions on a pasture. The decision tree models clearly revealed the interactions between the functional groups and environmental and management factors, and also indicated the relative importance of these factors in influencing the functional group abundance. Soil Olsen P was the most significant factor influencing the abundance of LFTG and moss, while soil bulk density, slope and annual P fertiliser input were the most significant factors influencing the abundance of legume, HFRG and flatweeds, respectively. Generally, slope and soil Olsen P were the two key factors underlying the patterns of abundance for these five functional groups. For the five functional groups studied, there was an overall predictive accuracy of 75%. Modelling functional group abundance simplified the investigation of the complex interrelationship between species and environment in a pasture ecosystem. The integration of the decision tree with GIS in this study provides a platform to investigate community structure and functional composition for a pasture over space, and thus can be applied as a tool in pasture management.     

COALBED METHANE PRODUCT WATER CHEMISTRY IN THREE WYOMING WATERSHEDS1

ABSTRACT: The Powder River Basin in Wyoming has become one of the most active areas of coalbed methane (CBM) development in the western United States. Extraction of methane from coalbeds requires pumping of aquifer water, which is called product water. Two to ten extraction wells are manifolded into one discharge point and product water is released into nearby unlined holding ponds. The objective of this study was to evaluate the chemistry, salinity, and sodicity of CBM product water at discharge points and associated holding ponds as a function of watershed. The product water samples from the discharge points and associated holding ponds were collected from the Cheyenne River (CHR), Belle Fourche River (BFR), and Little Powder River (LPR) watersheds during the summers of 1999 and 2000. These samples were analyzed for pH, electrical conductivity (EC), total dissolved solids (TDS), alkalinity, sodium (Na), calcium (Ca), magnesium (Mg), potassium (K), sulfate (SO₄^2‐), and chloride (C^1‐). From the chemical data, practical sodium adsorption ratio (SARp) and true sodium adsorption ratio (SARt) were calculated for the CBM discharge water and pond water. The pH, EC, TDS, alkalinity, Na, Ca, Mg, K, SARp, and SARt of CBM discharge water increased significantly moving north from the CHR watershed to the LPR watershed. CBM discharge water in associated holding ponds showed significant increases in EC, TDS, alkalinity, Na, K, SARp, and SARt moving north from the CHR to the LPR watershed. Within watersheds, the only significant change was an increase in pH from 7.21 to 8.26 between discharge points and holding ponds in the LPR watershed. However, the LPR and BFR exhibited larger changes in mean chemistry values in pH, salinity (EC, TDS), and sodicity (SAR) between CBM product water discharges and associated holding ponds than the CHR watershed. For instance, the mean EC and TDS of CBM product water in LPR increased from 1.93 to 2.09 dS/m, and froml,232 to 1,336 mg/L, respectively, between discharge and pond waters. The CHR exhibited no change in EC, TDS, Na, or SAR between discharge water and pond water. Also, while not statistically significant, mean alkalinity of CBM product water in BFR and LPR watersheds decreased from 9.81 to 8.01 meq/L and from 19.87 to 18.14 meq/L, respectively, between discharge and pond waters. The results of this study suggest that release of CBM product water onto the rangelands of BFR and LPR watersheds may precipitate calcium carbonate (CaCO₃) in soils, which in turn may decrease infiltration and increase runoff and erosion. Thus, use of CBM product water for irrigation in LPR and BFR watersheds may require careful planning based on water pH, EC, alkalinity, Na, and SAR, as well as local soil physical and chemical properties.