Differential fitness of allelic isozymes in the marine gastropodsLittorina punctata andLittorina neritoides,exposed to the environmental stress of the combined effects of cadmium and mercury pollution

The present study tested the separate and the interactive pollution effects of cadmium and mercury on the electrophoretically detected allelic isozyme frequencies of the enzyme phosphoglucose isomerase for two species of littoral marine gastropods —Littorina punctata andL. neritoides — and the enzyme amino peptidase forL. neritoides. Our results indicate differential survivorship of allelic isozyme genotypes specific for each type of pollutant and for their interaction, as well as trends common to all pollutants. Theoretically the results reflect the adaptive nature of at least some allozymic genotypes in these marine gastropods and seem inconsistent with the neutral theory of allozyme polymorphisms. Practically, the results reinforce earlier conclusions that changes in the frequency of allelic isozymes may be used as a genetic monitor of pollution.     

Ecophysiological constraints associated with aggression,and evolution toward pacifism in Spalax ehrenbergi

Evolution from a solitary way of life to sociality involves changes in the modalities of within-population aggression. This paper is concerned with environmental causes of variation in aggression within the complex of mole-rat species Spalax ehrenbergi. We focus on physiological constraints associated with aggressive behaviour, i.e. those resulting from emotional arousal and thus stress. Aggression, social stress sensitivity and urine osmolarity were studied in males of two chromosomal species of Spalax ehrenbergi, 2n = 52 (occurring in cool humid areas) and 2n = 60 (occurring in warm arid zones). Males with 2n = 52 reached a higher level of within-population aggression during a 3-round tournament (an average, 488 ± 140 acts) than males with 2n = 60 (163 ± 54 acts). The species with 2n = 52 displayed a higher corticosterone response after a social encounter (in average, 5.15 ± 0.54 μg/100 ml) than that with 2n = 60 (3.0 ± 0.3 μg/100 ml) whereas resting levels of this hormone were low and not different between the two species. Urine osmolarity was significantly higher in males with 2n = 60 (331.9 ± 18.6 mmol/kg) than males with 2n = 52 (267.3 ± 10.9 mmol/kg). Considering the overall general trend urine osmolarity tended to be negatively related to the level of aggression, while corticosterone levels tended to vary positively with aggressiveness (Fig. 3). Our results allow further discussion of the hypothesis that high levels of aggression could be selected against in arid habitats due to their physiological correlates (decrease in water economy, increased corticosterone leading to potential energy mobilisation and loss). We propose that behavioural ecophysiology could have contributed to the radiation of the S. ehrenbergi superspecies into increasingly arid environments, and may favour evolution toward social tolerance. Received: 10 June 1995/Accepted after revision: 25 October 1995     

Genetic variability in the marine gastropods Patella coerulea and Patella aspera: patterns and problems

Allozymic variation encoded by 15 gene loci was compared and contrasted in naturally coexisting populations of two marine gastropods (Patella coerulea and Patella aspera) collected along the rocky beaches of the Mediterranean sea of Israel in June, 1979. The genetic variability did not differ consistently or significantly between the two species, but in all the populations studied, P. aspera was more heterozygous than P. coerulea. These results are discussed in terms of the potential ecological factors affecting this genetic differentiation.     

Heavy metal selection of phosphoglucose isomerase allozymes in marine gastropods

The effects of zinc and copper pollution on the allozymic variation of phosphoglucose isomerase (Pgi) genotypes were tested in the Mediterranean marine gastropods Monodonta turbinata and M. turbiformis in 26 laboratory tests involving 2 081 adult individuals, with 545 survivors. Our results indicate differential survivorship of electrophoretical Pgi allozyme genotypes for both pollutants. Zinc pollution selected against the allozyme genotype SS in M. turbinata, whereas no differential survivorship was observed in M. turbiformis which lacks this allozyme. Copper pollution selected against the MM genotype in both species. These results are inconsistent with the neutral theory of allozyme polymorphisms and appear to reflect the adaptive nature of some Pgi genotypes in these marine gastropods.     

Species,population and individual specific odors in urine of mole rats (Spalax ehrenbergi) detected by laboratory rats

Summary Urine odors from 4 species of blind subterranean mole rats belonging to theSpalax ehrenbergi superspecies in Israel were evaluated to determine whether there were discriminable differences in the odors that were specific to different individuals, populations, and species of mole rats. Trained laboratory rats assessed these differences in an automated olfactometer using an operant conditioning paradigm. They demonstrated the discriminability of the urine odors in the 3 categories by their correct responses in generalization trials. These characteristic species, population and individual odors may provide these solitary, territorial and blind rodents a means for diverse chemical communication in spacing behavior, reproduction, species isolation and speciation.     

Motorcycle fuel tanks and pelvic fractures: A motorcycle fuel tank syndrome

Objective: Pelvic injuries are a serious and commonly occurring injury to motorcycle riders involved in crashes, yet there has been limited research investigating the mechanisms involved in these injuries. This study aimed to investigate the mechanisms involved in pelvic injuries to crashed motorcyclists.

Method: This study involved in-depth crash investigation and 2 convenience-based data sets were used. These data sets investigated motorcycle crashes in the Sydney, Newcastle, and Adelaide regions. Participants included motorcycle riders who had crashed either on a public road or private property within the study areas. The mechanism of injury and the type of injuries were investigated.

Results: The most frequent cause of pelvic injuries in crashed motorcyclists was due to contact with the motorcycle fuel tank during the crash (85%). For riders who had come into contact with the fuel tank, the injury types were able to be grouped into 3 categories based on the complexity of the injury. The complexity of the injury appeared to increase with impact speed but this was a nonsignificant trend. The pelvic injuries that did not occur from contact with the fuel tank in this sample differed in asymmetry of loading and did not commonly involve injury to the bladder. They were commonly one-sided injuries but this differed based on the point of loading; however, a larger sample of these injuries needs to be investigated.

Conclusion: Overall improvements in road safety have not been replicated in the amelioration of pelvic injuries in motorcyclists and improvements in the design of crashworthy motorcycle fuel tanks appear to be required.     

Discordant growth in twins

Discordant growth in twins contributes significantly to rates of perinatal morbidity and mortality. These rates vary according to chorionicity, timing of onset and severity. We have reviewed English language literature in Medline since 1980. It is clear that diagnosis of discordant growth has improved due to the use of serial ultrasound examination. Following the detection of differences in fetal size, diagnosis is facilitated by umbilical artery and fetal Doppler studies. Management options vary according to chorionicity, timing of onset and umbilical-fetal Doppler studies. The mode of delivery in discordant twins remains controversial. We conclude that ultrasound surveillance of twin gestations, combining serial biometry and selective Doppler studies, is effective in the recognition of siginificant intrauterine growth restriction in co-twins. Differences in etiology and management underscore the imortance of establishing chorionicity routinely as soon as twin gestation is diagnosed. Copyright © 2005 John Wiley & Sons, Ltd.