Biosynthesis of Poly(3-hydroxybutyrate) from Cheese Whey by <Emphasis Type="Italic">Bacillus megaterium</Emphasis> NCIM 5472

Microbial polyhydroxyalkonate such as homopolyester of poly(3-hydroxybutyrate) (PHB) was produced from cheese whey by Bacillus megaterium NCIM 5472. Due to their numerous potential industrial applications, the focus was given to competently enhance the amount of PHB produced. The amount of PHB produced from whole cheese whey, and ultrafiltered cheese whey was first compared, and after observing a rise in PHB production by using ultrafiltered cheese whey, cheese whey permeate was chosen for further analysis. The presence of PHB was then confirmed by GCMS. Since the main aim of the study was to increase the amount of PHB produced through batch fermentation, various process parameters like time, pH, C/N ratio, etc. were optimized. After optimization, it was found that B. megaterium NCIM 5472 was capable of accumulating 75.5% of PHB of its dry weight and a PHB yield of 8.29 g/L. The chemical structure of the polymer was further analyzed by using FTIR and NMR spectroscopy methods. Also, the physical and thermal properties were studied by using Differential scanning calorimetry and Thermogravimetric analysis. It was found that the polymer produced had excellent thermal stability, thus allowing the possibility to exploit its properties for industrial purposes such as adhesives, packaging materials, etc.     

Reproductive toxicity of lead,cadmium, and phthalate exposure in men

Environmental toxicants viz lead or cadmium and phthalate esters (di(2-ethylhexyl) phthalate [DEHP], dibutyl phthalate [DBP], and diethyl phthalate [DEP]) widely found in different environmental strata are linked to deteriorating male reproductive health. The objective was to assess the relationships between the seminal lead, cadmium, and phthalate (DEHP, DBP, DEP) concentrations at environmental level and serum hormone levels and semen quality in non-occupationally exposed men and specify the effect of individual and combined exposure of toxicants on semen quality. A study of 60 male partners of couples attending the Andrology Laboratory of the Reproductive Biology Department, All India Institute of Medical Sciences (AIIMS), New Delhi, India for semen analysis to assess their inability to achieve a pregnancy was selected for the study. The results of univariate and stepwise multiple regression analysis in the unadjusted model showed a significant correlation between lead or cadmium and phthalates DEHP/DBP/DEP and sperm motility, sperm concentration, and DNA damage. After adjusting for potential confounders, an association with lead or DEHP was only observed. The present data shows that lead (Pb) or cadmium (Cd) or phthalates might independently contribute to decline in semen quality and induce DNA damage. Phthalates might influence reproductive hormone testosterone. These findings are significant in light of the fact that men are exposed to a volley of chemicals; however, due to the small sample size, our finding needs to be confirmed in a larger population.     

Indoor–outdoor concentrations of RSPM in classroom of a naturally ventilated school building near an urban traffic roadway

A study on indoor–outdoor RSPM (PM₁₀, PM_2.5 and PM_1.0) mass concentration monitoring has been carried out at a classroom of a naturally ventilated school building located near an urban roadway in Delhi City. The monitoring has been planned for a year starting from August 2006 till August 2007, including weekdays (Monday, Wednesday and Friday) and weekends (Saturday and Sunday) from 8:0 a.m. to 2:0 p.m., in order to take into account hourly, daily, weekly, monthly and seasonal variations in pollutant concentrations. Meteorological parameters, including temperature, rH, pressure, wind speed and direction, and traffic parameters, including its type and volume has been monitored simultaneously to relate the concentrations of indoor–outdoor RSPM with them. Ventilation rate has also been estimated to find out its relation with indoor particulate concentrations. The results of the study indicates that RSPM concentrations in classroom exceeds the permissible limits during all monitoring hours of weekdays and weekends in all seasons that may cause potential health hazards to occupants, when exposed. I/O for all sizes of particulates are greater than 1, which implies that building envelop does not provide protection from outdoor pollutants. Further, a significant influence of meteorological parameters, ventilation rate and of traffic has been observed on I/O. Higher I/O for PM₁₀ is indicating the presence of its indoor sources in classroom and their indoor concentrations are strongly influenced by activities of occupants during weekdays.     

MTBE oxidation byproducts from the treatment of surface waters by ozonation and UV-ozonation

In recent years, there has been considerable concern over the release of methyl tert-butyl ether (MTBE), a gasoline additive, into the aquifers used as potable water sources. MTBE readily dissolves in water and has entered the environment via gasoline spills and leaking storage tanks. In this paper, we investigate ozonation and UV-ozonation for treatment of MTBE in contaminated drinking water sources. We report the test protocol and results of using solid-phase microextraction (SPME) to determine the level of MTBE and its oxidation byproducts in samples drawn from laboratory-scale ozone and UV-ozone reactors being evaluated at a US EPA research facility. Analysis of a prepared MTBE standard indicated a detection limit on the order of 0.1 microgl(-1) with a repeatability of +/-0.4%. Results show that the overall rate of removal of MTBE via UV-ozonation in a relatively turbid surface water (15 ntu) is twice that of ozonation alone. In addition, GC-MS analysis of decomposition products showed that tert-butyl formate (TBF), methyl acetate, butene, acetone, and acetaldehyde were produced by both processes. TBF and butene reach similar maximum yields from the two processes, but are more efficiently degraded by UV-ozonation treatment. This indicates that these treatment processes also degrade these byproducts. In contrast, the remaining byproducts (methyl acetate, acetone, and acetaldehyde) are formed at similar levels during treatment, but are not degraded once formed. These byproducts may be resistant to hydrogen abstraction by hydroxyl radical.     

Toxicity and uptake of mercurials in a cyanobacterium

Nostoc calcicola cells exposed to mercuric chloride (0.05–0.25 M), methyl mercuric chloride (0.05–0.15 M) and the fungicide ceresan (phenyl mercuric acetate; 0.05–0.20 M) showed sensitivity in the sequence: methyl mercury3) over phenyl mercuric acetate (0.51×10³); inorganic mercury occupied the intermediate position with a bioconcentration factor of 1.32×10³. The data infer that larger molecules of organomercurials may not be taken up by cells at the rate and extent comparable to the smaller species.     

Formulation of a landfill pollution potential index to compare pollution potential of uncontrolled landfills

Generally speaking, landfilling is one of the prominent methods of waste disposal around the globe, but some under-developed and developing countries still continue to practice uncontrolled open dumping of waste. These uncontrolled landfills pose a relatively high threat to the various elements of the environment in comparison with the conventional engineered landfills that are used in many developed countries. However, some closed, un-engineered landfills do exist in developing countries. This paper presents a novel approach to compare the pollution potential of uncontrolled landfills using an index. The landfill pollution potential index (LPPI) has been developed using the Delphi technique and is an aggregation of six pollution indices that have already been developed for the quantification of different environmental elements. The LPPI is an increasing scale index, in which a higher index value indicates a higher pollution threat. The LPPI of a landfill in Delhi was calculated and the high LPPI value indicates that the respective landfill poses a significant threat to the environment. The LPPI can be used as an aid to diagnose a landfill's pollution potential relative to other landfills and therefore also to rank remediation investments.     

Origin and nature of spermatophoric mass of the spiny lobster Panulirus homarus

In the spiny lobster Panulirus homarus (Linnaeus) the spermatophoric mass consists of a highly convoluted tube, containing the sperm mass and a gelatinous matrix. The spermatophoric components have their origin in the proximal vas deferens and the completed spermatophoric mass is stored within the distal dilated part of the vas deferens. In the proximal vas deferens the inner glandular epithelial cells give rise to leaf-like typhlosoles which gradually diminish in size and finally disappear in the distal vas deferens. The secretions originating from the typhlosole form the outer gelatinous matrix of the spermatophoric mass. Histochemical observations reveal that the wall of the spermatophore tube consists of neutral mucopolysaccharide, whereas the sperm mass and the gelatinous matrix are rich in acidic mucopolysaccharides. Both polyacrylamide gel electrophoresis and agarose gel electrophoresis were used to separate the acidic mucopolysaccharide (AMPS) complexed with the proteins and the simple proteins. In addition, AMPS have been isolated following the method of Rahemtulla and Lovtrup (1974) and used for electrophoresis. In both the polyacrylamide gel electrophoresis and agarose gel electrophoresis, the extracted acid mucopolysaccharides give only a single fraction which, in comparison with the standard acid mucopolysaccharides, corresponds to chondroitin sulphate. The functional significance of the chondroitin sulphate and neutral mucopolysaccharides in the spermatophoric components of P. homarus is discussed in relation to their functional role in spermatophore hardening and protection of the delicate spermatozoa during their prolonged storage on the sternum of the female.