Use of native mosses as biomonitors of heavy metals and nitrogen deposition in the surroundings of two steel works

A biomonitoring survey using the moss species Hypnum cupressiforme Hedw. was conducted in the surroundings of two steel plants located in the North of Spain. Levels of V, Cr, Ni, Cu, Zn, As, Cd, Hg, Pb and N were determined. Very high concentrations in the areas of study were detected when compared to nearby unaffected regions. Similar trends were observed for all the elements in the differently orientated transects, showing an appreciable influence of the NW prevailing winds of the region in the dispersion of pollutants, as well as a clear decreasing gradient in the concentrations of metals in mosses within a distance of 1500 meters from the facilities. A differentiation between the elements emitted by the chimney as result of the industrial activity (V, Cr, Ni, Cu and As) and those with a high presence in steel slag deposits (Zn, Cd, Hg and Pb) was observed. The range of contamination was also established by means of the Contamination Factor, indicating a category 4 out of 6 categories, which shows the high levels reported in the areas of study. A different dynamic was registered for nitrogen regarding the rest of the heavy metals analysed except for Hg, probably due to the elevated volatility and mobility of both elements, as well as their high persistence in the atmosphere.     

Twenty-five years of HAZOPs

The HAZOP or hazard and operability study was developed by ICI over 25 years ago and is in wide use today throughout the world. The technique is intended to review, in a formalized manner, the design of a system such as a chemical process production plant to detect potential problems in the operation of the system. The system has been extended for use on all types of production, storage and distribution units. Experience in the use of hazard studies has lead to the development of a phased approach with three distinct design phases.     

An exploratory study with the proton microprobe of the ontogenetic distribution of 16 elements in the shell of living oysters (Crassostrea virginica)

Study of the chemical composition of shell of exoskeletonous organisms in the past has required the sacrifice of the organism. Because the beam of the proton microprobe is relatively nondestructive and analyzes the surface layer of the shell, organisms do not have to be killed. The present paper presents results of a preliminary experiment in which distribution of elements (Na to Sr) in shell of living juvenile oysters, Crassostrea virginica (Gmelin), was studied in situ with a proton microprobe at monthly intervals for four months. The relative concentration of 16 elements was measured in the newly deposited prismatic edge of the right valve of three oysters reared in controlled laboratory conditions. Na, Mg, Al, Si, S, Cl, K, Ca, Ti, Cr, Mn, Fe, Cu, Zn, Br, and Sr were detected in concentrations as low as a few parts per million relative to the concentration of standards added to pure CaCO₃. Concentration of elements varied nominally among shells of the three individual oysters and in their successive ontogenetic stages. Fluctuations in concentration of Na, Mg, S, Cl, Ca, Mn, Fe, Cu, and Zn were generally similar in the two normally growing oysters, but differed from those in the oyster that stopped growing. Trends in concentration of Al, Si, and Sr were similar in the three oysters: those of Br were variable. Relative concentrations of Na, Cl, S, Mn, Fe, and Zn increased slightly with age of oysters, that of the other elements stayed relatively constant. Concentration of most elements was higher in shell than in seawater. Variable concentrations, especially of Na, Cl, and Si in valve edges, tend to support the hypothesis of earlier workers that separate mineral phases are present as impurities entrapped within the shell during calcification.     

The effects of benomyl and its breakdown products carbendazim and butyl isocyanate on the structure and function of tracheal ciliated cells

Abstract

The effects of the fungicide benomyl and its breakdown products, carbendazim and butyl isocyanate, were examined on canine tracheal epithelial tissue in primary culture. Changes in ciliary frequencies were monitored with an optical spectrum analysis system. Serial dilutions of the test compounds were prepared in 100% corn oil and applied to the cell cultures for intervals up to 6 hours and frequencies measured at intervals of 15 minutes to 1 hour. Benomyl and butyl isocyanate caused concentration‐dependent decreases in ciliary beat frequency. Benomyl at 300 μg/ml (3 mM) caused ciliostasis within 75 minutes of exposure. Butyl isocyanate at a molar concentration three times lower than benomyl (1 mM) caused a similar response, although within 30 minutes. The IBC₅₀ for benomyl was 0.75 mM, while for butyl isocyanate it was 0.52 mM. Carbendazim caused a moderate decrease in frequency over a 6 hour exposure period. Benomyl caused moderate to severe swelling of the mitochondria of ciliated epithelial cells with other cell organelles appearing normal. Butyl isocyanate did not cause any noticeable effect on cell ultrastructure and the apparently low rate of penetration of carbendazim into cells made it impossible to obtain an effect which justified ultrastructural analysis. It appears, at least for benomyl and butyl isocyanate, that while the physiological effect of these two compounds (inhibition of ciliary beat) is the same, the sites of action in the cell may be different.     

An assessment of coastal land-use and land-cover change from 1974–2008 in the vicinity of Mobile Bay,Alabama

The purpose of this research is to quantify and assess geospatial land-use and land-cover (LULC) changes in the coastal counties of Mobile and Baldwin, Alabama using nine Landsat images from 1974–2008. A study-specific classification scheme was devised comprising upland herbaceous, upland forest, non-woody and woody wetlands, open water, and urban categories. Upland forest was the most dominant terrestrial cover type. Wetlands averaged 17% and urban averaged 7%. A majority of the urban expansion occurred between 1974 and 1979 (26%). Thirty-four percent of the 2008 urban areas were upland forest in 1974. Watershed-scale analysis of Three Mile Creek and D’Olive Bay highlights the temporal and spatial differences of urbanization for watersheds found within the same region. This study is a Gulf of Mexico Alliance (GOMA) Application Pilot project that uses NASA data products to benefit coastal environmental managers and community members. Results have led to increased effectiveness of coastal conservation decision-making, increased understanding of post-hurricane LULC change, continued research on habitat change impacts, and contributed to timely conservation planning efforts. This study has benefited the development of watershed management plans by the Mobile Bay National Estuary Program, which is especially important given projected climate change.     

The development and application of remote sensing to monitor sand dune habitats

Sand dunes are complex systems that contain several habitats, often as mosaics or transitions between types. Several of these habitats are afforded protection under European Legislation and in the UK nationally within Special Areas of Conservation (SAC) and Sites of Special Scientific Interest (SSSI). Natural England has a statutory duty to report to Europe on the conservation status and condition of sand dunes; and is required to report to the UK Government on designated sites. To achieve this we have sought ways of capturing, analysing and interpreting data on the extent and location of sand dune habitats. This requires an ability to be able to obtain data over large areas of coastline in an efficient way. Natural England and Environment Agency Geomatics have worked collaboratively for over 16 years, sharing data and ecological knowledge. In 2012 work started to evaluate the use of remote sensing to map UK BAP and Annex I sand dune habitats. A methodology has now been developed and tested to map sand dune habitats. The key objective was to provide an operational tool that will help to map these habitats and understand change on sites around England. This has been achieved through analysis of LIDAR and Compact Airborne Spectrographic Imager (CASI) data using Object Orientated Image Analysis. Quality Control (QC) and accuracy assessments have shown this approach to be successful and 11 sites have been mapped to date. These techniques are providing a new approach to monitoring change in coastal vegetation communities and informing management of protected sites.     

Chemical elements in the aragonitic and calcitic microstructural groups of shell of the oysterCrassostrea virginica: A proton probe study

We report the results of a study with proton-induced X-ray emissions (PIXE) of the distribution and concentration of 15 chemical elements (Na to Sr in periodic chart) in four microstructural and two mineralogical regions of shell of rapidly growing adult oysters [Crassostrea virginica (Gmelin)]. Hatchery-raised oysters were grown in Broadkill Estuary, Delaware, USA, for 16 wk in summer 1978. Their valve edges were filed as a marker, and the oysters were replaced in the estuary, where they grew rapidly. Shell deposited after marking had a normal microstructure and mineralogy after a narrow zone of disturbance. After 17 d oysters were sacrificed, and different mineralogical and microstructural regions of valves of three oysters, and parts of valves of two oysters, were analyzed for the elements Na to Sr. Quadrats (2.5 × 0.45 mm) included three calcitic groups (prismatic, foliated, chalky) and one aragonitic (myostracal) microstructural group; four quadrats were on the exterior and five on the interior of right and left valves. Inhalant and exhalant margins of valves and ground right and left valves of one oyster were also analyzed. Elemental chemistry of different regions of shell varied among the three microstructural groups within the single calcitic polymorph, between aragonitic and calcitic regions, and between exhalant and inhalant margins of the valves. Elements were most concentrated in the prismatic region of the right valve. Element concentrations were similar in ground right and left valves, except for higher levels of Si, Fe, and Zn in the right valve (corresponding to their high contents in prismatic shell) and of Cl in the left valve (reflecting high concentration in chalky shell, abundant in this valve). Na, Mg, Cl, Cr, Cu, Zn and Br were more concentrated in prismatic than in foliated shell. Chalky shell contained higher concentrations of Na than did prismatic shell, and high concentrations (but lower than in prismatic shell) of Mg, Cl, Ti, Mn, Fe, Zn and Br. Element concentration in myostracum was approximately the same as, or lower than, in foliated shell, except for Sr, which was higher than that in any other shell group. In the right valve most elements were concentrated in inhalant margins, and on the left valve, in exhalant margins. With increased weathering of the exterior surface of prismatic shell, Mg, Si, and Mn increased in concentration and Na, Al, Cl, Ti, Cr, Fe, Br, and Sr decreased.