Fate of bisphenol A during treatment with the litter-decomposing fungi Stropharia rugosoannulata and Stropharia coronilla

Bisphenol A is an endocrine disrupting compound, which is ubiquitous in the environment due to its wide use in plastic and resin production. Seven day old cultures of the litter-decomposing fungus Stropharia coronilla removed the estrogenic activity of bisphenol A (BPA) rapidly and enduringly. Treatment of BPA with purified neutral manganese peroxidase (MnP) from this fungus also resulted in 100% reduction of estrogenic activity, as analyzed using a bioluminescent yeast assay, and in the formation of polymeric compounds. In cultures of Stropharia rugosoannulata, estrogenic activity also quickly disappeared but temporarily re-emerged in the further course of cultivation. LC-MS analysis of the extracted estrogenic culture liquid revealed [M−H]⁻ ions with m/z values of 219 and 235. We hypothesize that these compounds are ring fission products of BPA, which still exhibit one intact hydroxyphenyl group to interact with estrogen receptors displayed by the yeast.     

Circular Makerspaces: the founder’s view

Abstract

Makerspaces – open access design and fabrication workshops – provide new contexts for design practice through ‘distributed production’. The global community of makerspaces has evolved quickly and in turn, substantial hype is attributed to its potential for radical sustainable innovation. In this article, we explore this potential in the context of the new ‘circular economy’ agenda. We focus the research on the critical role of makerspace managers/founders who are recognised as gatekeepers to circular practices. The research method is action-led including expert interviews (academics and founders/managers) as well as two generative context-mapping workshops, run at selected makerspaces in collaboration with their founders/managers. We unearth everyday ‘how-to’ guidance to interweave circular practices within makerspaces from the outset by: fostering an enabling culture; building local connections; nurturing individual/community capacities; and stimulating practical know-how. However, while the research reveals immense opportunities to cultivate circular literacy from within makerspaces, the prospects to ask more profound questions about our economy, through makerspace practices, are found to be compromised by day-to-day concerns. The insights from this research can act as a starting point for future work in this emerging research area.     

Evaluation of sites for the location of WEEE recycling plants in Spain

As a consequence of new European legal regulations for treatment of waste electrical and electronic equipment (WEEE), recycling plants have to be installed in Spain. In this context, this contribution describes a method for ranking of Spanish municipalities according to their appropriateness for the installation of these plants. In order to rank the alternatives, the discrete multi-criteria decision method PROMETHEE (Preference Ranking Organisation METHod for Enrichment Evaluations), combined with a surveys of experts, is applied. As existing plants are located in North and East Spain, a significant concentration of top ranking municipalities can be observed in South and Central Spain. The method does not present an optimal structure of the future recycling system, but provides a selection of good alternatives for potential locations of recycling plants.