Improved xenobiotic-degrading activity of Rhodococcus opacus strain 1cp after dormancy

The goals of the present work were as follows: to obtain the dormant forms of R. opacus 1cp; to study the phenotypic variability during their germination; to compare phenotypic variants during the growth on selective and elective media; and to reveal changes in the ability of the strain to destruct xenobiotics that had not been degradable before dormancy. It was shown that Rhodococcus opacus 1cp (the strain degrading chlorinated phenols) became able to utilize a broader spectrum of xenobiotics after storage in the dormant state. Germination of the dormant forms of R. opacus 1cp on an agarized medium was followed by emergence and development of phenotypic variants that could grow on 4-chlorophenol and 2,4,6-trichlorophenol without adaptation. The cells of R. opacus 1cp phenotypic variants also utilized all of the tested chlorinated phenols: 2,3-, 2,5-, and 2,6-dichloro-, 2,3,4- and 2,4,5-trichloro-, pentachlorophenol, and 1,2,4,5-tetrachlorobenzene in concentrations up to 60 mg/L, though at the lower rates than 4-CP and 2,4,6-TCP. The improved degradation of chlorinated phenols by R. opacus strain 1cp exposed to the growth arrest conditions demonstrates the significance of dormancy for further manifestation of the adaptive potential of populations. A new principle of selection of variants with improved biodegradative properties was proposed. It embraces introduction of the dormancy stage into the cell life cycle with subsequent direct inoculation of morphologically different colonies into the media with different toxicants, including those previously not degraded by the strain.     

Microbial conversion of fungicide vinclozolin

An ecological safety study of using vinclozolin in field and laboratory experiments showed that the effect of the preparation led to a decrease in the abundance of actinomycetes and mycelial fungi and an enhancement of nitrification. The residual amounts of vinclozolin in soil after 12 months were 6-12% of the dose introduced. The persistent chlorinated derivatives of the toxicant were found. Microbial strains pertaining to the genera Pseudomonas and Bacillus were isolated that utilized vinclozolin as the sole source of carbon and energy.     

Biodiversity Differences between Managed and Unmanaged Forests: Meta-Analysis of Species Richness in Europe

Abstract: Past and present pressures on forest resources have led to a drastic decrease in the surface area of unmanaged forests in Europe. Changes in forest structure, composition, and dynamics inevitably lead to changes in the biodiversity of forest‐dwelling species. The possible biodiversity gains and losses due to forest management (i.e., anthropogenic pressures related to direct forest resource use), however, have never been assessed at a pan‐European scale. We used meta‐analysis to review 49 published papers containing 120 individual comparisons of species richness between unmanaged and managed forests throughout Europe. We explored the response of different taxonomic groups and the variability of their response with respect to time since abandonment and intensity of forest management. Species richness was slightly higher in unmanaged than in managed forests. Species dependent on forest cover continuity, deadwood, and large trees (bryophytes, lichens, fungi, saproxylic beetles) and carabids were negatively affected by forest management. In contrast, vascular plant species were favored. The response for birds was heterogeneous and probably depended more on factors such as landscape patterns. The global difference in species richness between unmanaged and managed forests increased with time since abandonment and indicated a gradual recovery of biodiversity. Clearcut forests in which the composition of tree species changed had the strongest effect on species richness, but the effects of different types of management on taxa could not be assessed in a robust way because of low numbers of replications in the management‐intensity classes. Our results show that some taxa are more affected by forestry than others, but there is a need for research into poorly studied species groups in Europe and in particular locations. Our meta‐analysis supports the need for a coordinated European research network to study and monitor the biodiversity of different taxa in managed and unmanaged forests.     

The Evolution and Role of Non-OPEC Production in the International Oil Market

In 1982, for the first time since OPEC was founded in 1961, its production was surpassed by the non-OPEC countries in the world excluding the centrally planned economies. In that year, for the world as a whole, OPEC provided 35% of the oil consumed. The decline was brought about by a decrease in consumption and by a marked increase in production from non-OPEC sources. It is expected that non-OPEC producers will have the capacity to meet more than half of the world's oil requirements until the early 1990s, at which time OPEC producers will again assume the role of providing over 50% of the world's oil.     

Effect of alkyl hydroxybenzenes on the properties of dioxygenases

The aim of the present work was to investigate the influence of alkylhydroxybenzenes (AHBs) and tyrosol, which belong to cell differentiation factors d(1) group of autoregulators on properties of biodegradation enzymes, catechol 1,2-dioxygenase (Cat 1,2-DO) and methylcatechol 1,2-dioxygenase (MCat 1,2-DO) of Rhodococcus opacus 6a. AHBs were found to have a greater effect on MCat 1,2-DO than on Cat 1,2-DO. It was expressed by more pronounced changes in the activity of MCat 1,2-DO with unsubstituted catechol at different AHB concentrations and by increasing thermostability of MCat 1,2-DO compared to Cat 1,2-DO under the protective action of AHBs. The compound C(7)-AHB shifted the maximum of dioxygenase activities towards higher temperatures and increased their operation optimum. AHBs changed the specificity constant of dioxygenases by decreasing/increasing the K(m)/V(max) value. For example, the increase in the V(max) value of 3,6-dichlorocatechol oxidation by Cat 1,2-DO in the presence of C(7)-AHB was 300-fold higher compared to the same reaction without AHB. The influence of cell differentiation factors on the properties of dimeric enzymes has been shown for the first time. It gives an idea of how the specificity of enzymes can be changed in vivo when strains contact new substrates. The work has shown the possibility of modification of the properties of dimeric enzymes towards the extension of enzyme activity with difficulty converted substrates or in more extreme conditions, which may be important for biotechnological processes.     

Variability of enzyme system of Nocardioform bacteria as a basis of their metabolic activity

The present review describes some aspects of organization of biodegradative pathways of Nocardioform microorganisms, first of all, with respect to their ability to degrade aromatic compounds, mostly methylbenzoate, chlorosubstituted phenols, and chlorinated biphenyls and the intermediates of their transformation: 4-chlorobenzoate and para-hydroxybenzoate. Various enzyme systems induced during degradation processes are defined. The ability of microorganisms to induce a few key enzymes under the influence of xenobiotics is described. This ability may increase the biodegradative potential of strains allowing them to survive in the changing environment or demonstrate to some extent the unspecific response of microorganisms to the effect of toxicants. Nocardioform microorganisms responsible for degradation of such persistent compounds as polychlorinated biphenyls, polyaromatic hydrocarbons, chlorinated benzoates and phenols and other xenobiotics are characterized. The possibility of using Nocardioform microorganisms in some aspects of biotechnology due to their ability to produce some compounds important for industry is also estimated.     

渤海湾两条河流及其近岸海域水体中DOM的光谱特征及影响因素

应用三维荧光光谱结合吸收光谱分析,研究了2019年夏季天津市大清河-独流减河水系和河北省黄骅市捷地减河及其河口区水体中溶解有机物（DOM）的空间分布特征、来源及其影响因素。紫外-可见光谱结果表明,研究区水体的SUVA₂₅₄与盐度有显著正相关关系;两条河流的下游水体中S_275-295均值均大于上游和中游。平行因子分析结果表明,两条河流均检测到两种类腐殖质组分C1（240,300/390 nm）和C3（260,335/477 nm）以及一种类蛋白质组分C2（225,275/339 nm）。在大清河-独流减河水系及其近岸海域水体中,这三种组分与盐度呈显著负相关关系,其中由于人为输入（TN和TP含量高,工业排污）影响导致C2与盐度的相关性弱于C1和C3。该水系水体在低盐度时C2的占比高于捷地减河,主要受到工农业废水输入与其支流子牙河汇入的共同影响,而捷地减河主要受农业废污水输入的影响。此外,自然因素（盐度、海水混合）和人为因素（人为输入、筑坝拦截）的不同导致渤海湾两条入海河流中有色溶解有机质（CDOM）的光学特性和混合行为出现显著差异。其中筑坝拦截导致独流减河坝前水体的CDOM平均分子量低于下游水体,并且增强了河口区陆源类腐殖质的去除作用。综上,三维荧光光谱结合平行因子分析能判别河流局部条件的差异对整体CDOM空间分布特征的影响,研究结果可为渤海湾地区河流及河口水体CDOM生物地球化学过程研究提供基础数据。     

Transformation of the insecticide teflubenzuron by microorganisms

Transformation of teflubenzuron, the active component in the insecticide commercialized as Nomolt, by soil microorganisms was studied. It was shown that microorganisms, belonging to Bacillus, Alcaligenes, Pseudomonas and Acinetobacter genera are capable to perform the hydrolytic cleavage of the phenylurea bridge of teflubenzuron in different positions, especially active was Bacillus brevis 625. The structure of the intermediates formed was established using TLC, HPLC, mass-spectrometry and 19F NMR techniques. It was shown that for a dose range of 53-132 microM and upon 12 days of fermentation about 30% of the teflubenzuron was modified. About 10-15% was transformed into 2,6-difluorobenzamide, 3-5% into 2,6-difluorobenzoic acid, 10-12% into 2,4-difluoro-3,5-dichloro-aniline. The late compound gave rise to formation of a condensed compound, identified as 1,2-bis(2,4-difluoro-3,5-dichlorophenyl)urea with molecular mass of 420. The results obtained indicate degradation of teflubenzuron by soil microorganisms to be a process to be mediated by microbial consortia, and starting with hydrolysis of the phenylurea bridge by several bacterial species. Subsequent further degradation of the aromatic degradation products has to be mediated by other strains known to be capable of degradation of halogenated aromatics.     

Improved xenobiotic-degrading activity of Rhodococcus opacus strain 1cp after dormancy

The goals of the present work were as follows: to obtain the dormant forms of R. opacus 1cp; to study the phenotypic variability during their germination; to compare phenotypic variants during the growth on selective and elective media; and to reveal changes in the ability of the strain to destruct xenobiotics that had not been degradable before dormancy. It was shown that Rhodococcus opacus 1cp (the strain degrading chlorinated phenols) became able to utilize a broader spectrum of xenobiotics after storage in the dormant state. Germination of the dormant forms of R. opacus 1cp on an agarized medium was followed by emergence and development of phenotypic variants that could grow on 4-chlorophenol and 2,4,6-trichlorophenol without adaptation. The cells of R. opacus 1cp phenotypic variants also utilized all of the tested chlorinated phenols: 2,3-, 2,5-, and 2,6-dichloro-, 2,3,4- and 2,4,5-trichloro-, pentachlorophenol, and 1,2,4,5-tetrachlorobenzene in concentrations up to 60 mg/L, though at the lower rates than 4-CP and 2,4,6-TCP. The improved degradation of chlorinated phenols by R. opacus strain 1cp exposed to the growth arrest conditions demonstrates the significance of dormancy for further manifestation of the adaptive potential of populations. A new principle of selection of variants with improved biodegradative properties was proposed. It embraces introduction of the dormancy stage into the cell life cycle with subsequent direct inoculation of morphologically different colonies into the media with different toxicants, including those previously not degraded by the strain.     

The fate of methoxychlor in soils and transformation by soil microorganisms

Abstract

Methoxychlor was found to be sufficiently persistant in soil and its residues were present even 18 months after the soil treatment. Saprophytes, fungi and actinomyces were unaffected by varying concentrations of methoxychlor, azotobacter however was susceptable. Soil strains isolated did not utilize methoxychlor as a sole carbon source except for 9 cultures belonging to the genera Bacillus, Acineto‐bacter and Rhodococcus which carried out the complete dechlorination, demethylation and splitting of one of methoxychlor aromatic rings. Anaerobic conditions were more favorable for methoxychlor biodegradation by soil and pure microbial cultures.