Expression of copper-resistance genes in microbial communities under copper stress and oxic/anoxic conditions

Microorganisms have developed copper-resistance mechanisms in order to survive in contaminated environments. The abundance and expression of the copper-resistance genes cusA and copA, encoding respectively for a Resistance Cell Nodulation protein and for a P-type ATP-ase pump, was assessed along a gradient of copper concentration in microcosms prepared from Seine estuary mudflat sediment. We demonstrated that the abundance of copA and cusA genes decreased with the increase of copper concentration and that cusA gene was up to ten times higher than the copA gene. Only the copA gene was expressed in both oxic and anoxic conditions. The abundance and activity of the microbial community remained constant whatever the concentrations of copper along the gradient. The molecular phylogeny of the two copper-resistance genes was studied and revealed that the increase of copper increased the diversity of copA and cusA gene sequences.     

Trends in cyberspace: can governments keep up?

Whether carried out by individuals or states, cyberattacks are both growing in number and becoming more sophisticated. Since the attack on Estonian cyber infrastructure in 2007, many other examples of massive attacks have been reported. The use of spyware and malware—such as with Stuxnet, DuQu or Flames—to disrupt critical infrastructure has made headlines, questioning the ability of governments and private actors to respond to cyber threats. A broad array of potential threats poses a substantial challenge to existing governance structures, which are often behind the curve in comparison with the dynamically evolving cyberspace. Using existing literature and recent foresight studies, the article analyses the trends in the governance of cyberspace and their implications for governments and global regulatory regimes.     

Application of fuzzy logic to explosion risk assessment

Safety and health of workers potentially being at risk from explosive atmospheres are regulated by separate regulations (ANSI/AIHA in USA and ATEX in the European Union). The ANSI/AIHA does not require risk assessment whereas it is compulsory for ATEX. There is no standard method to do that assessment. For that purpose we have applied the explosion Layer of Protection Analysis (ExLOPA), which enables semi-quantitative risk assessment for process plants where explosive atmospheres occur. The ExLOPA is based on the original work of CCPS for LOPA taking into account an explosion accident scenario at workplace. That includes typical variables appropriate for workplace explosion like occurrence of the explosive atmosphere, the presence of effective ignition sources, activity of the explosion prevention and mitigation independent protection layers as well as the severity of consequences. All those variables are expressed in the form of qualitative linguistic categories and relations between them are presented using expert based engineering knowledge, expressed in the form of appropriate set of rules. In this way the category of explosion risk may be estimated by the semi-quantitative analysis. However, this simplified method is connected with essential uncertainties providing over or under estimation of the explosion risk and may not provide real output data.In order to overcome this problem and receive more detailed quantitative results, the fuzzy logic system was applied. In the first stage called fuzzification, all linguistic categories of the variables are mapped by fuzzy sets. In the second stage, the number of relation between all variables of analysis are determined by the enumerative combinatorics and the set of the 810 fuzzy rules “IF-THEN” is received. Each rule enables determination of the fuzzy risk level for a particular accident scenario. In the last stage, called defuzzification, the crisp value of final risk is obtained using a centroid method. The final result of the risk presents a contribution of each risk category represented by the fuzzy sets (A, TA, TNA and NA) and is therefore more precise and readable than the traditional approach producing one category of risk only. Fuzzy logic gives a possibility of better insights into hazards and safety phenomena for each explosion risk scenario. It is not possible to receive such conclusions from the traditional ExLOPA calculation results. However it requires the application of computer-aided analyses which may be partially in conflict with a simplicity of ExLOPA.The practical example provides a comparison between the traditional results obtained by ExLOPA and by fuzzy ExLOPA methods.     

Microfiber shedding from nonwoven materials including wipes and meltblown nonwovens in air and water environments

Environmental Science and Pollution Research - Nonwoven products are widely used in disposable products, such as wipes, diapers, and masks. Microfibers shed from these products in the aquatic and...