光合细菌与酵母跨界融合(杂合)子发酵味精废水研究

光合细菌球形红假单胞菌（Ｒｈｏｄｏｐｓｅｕｄｏｍｏｎａｓ ｓｐｈａｅｒｏｉｄｅｓ）酿酒酵母（Ｓａｃｃｈａｒｏｍｙｃｅｓ ｃｅｒｅｖｉｓｉａｅ）细胞分别经溶菌酶和蜗牛酶脱壁形成原生质体，在聚乙二醇（ＰＥＧ）和Ｃａ＾２＋诱导下实现了真核与原核细胞的超远缘杂交，经抗菌素培养基初步鉴定，融合产物能同时在含有链霉素和制霉菌素的培养基中生长，而双亲细胞只能在含有单一抗菌素的培养基中生长，经初筛与得获得高絮弟性     

味精废水中重金属及金属污染对处理利用的影响

本文应用等离子光谱（ＩＣＰ）测试三个厂家味精生产废水中２１种金属元素的水平，其中金属元素的总水平分别达１９１９２ｍｇ．ｌ＾－１，３１９０ｍｇ．ｌ＾－１和３１６１ｍｇ．ｌ＾－１，对照国内外有关的四项水质标准，三厂家味精生产废水中存在着Ｆｅ，Ｚｎ等多种重金属或其他金属元素严重超标现象，味精生产废水对青芋存在急性毒性，２４，４８，７２和９６ｈ的半致死味精废水浓度ＬＣ５０分别为６．９％，３．２％，２．５％     

光合细菌与酵母跨界融合子降解味精废水性能测定

为了筛选出能综合双亲优势的杂种细胞,为废资源化生产ＳＣＰ提供更为理想的菌株来源,将原核球形红假单胞菌细胞与真核酿酒酵母细胞的原生质体融合,构建成跨界杂种细胞。从中筛选出Ｆ１３,Ｆ１５,Ｆ２０三株高絮凝性融合细胞,在起始ＢＯＤ５浓度为１９１－６９０ｍｇ／Ｌ,ｐＨ７．０,３０℃的味精废水中振荡反应７－８ｈ测得融合细胞双亲菌株降解利用有机污染物的系列的反应动力学参数μｍａｘ,ｑｍａｘ,Ｋｓμ,Ｋｓｑ,Ｙ     

麦草畏除草剂厌氧降解可生化性测定

苯环由¹⁴C标记合成的麦草畏除草剂,厌氧降解反应至第7天,转化率达64%,有¹⁴C标记的CO₂产生;第16天,转化率达91%,出现一种主要的中间代谢产物,有CH₄产生;第27天,转化率达95%,仍然只有一种主要的中间代谢产物.麦草畏苯环的断裂量仅占总量的2.96%,转化成CO₂的为0.74%,转化成CH₄的为0.11%.在降解27d中,主要中间代谢产物是3,6-二氯水杨酸,占麦草畏总量的92.04%.3,6-二氯水杨酸的苯环没有被打开,可生化性极低,仍然继续污染环境研究结果为控制麦草畏对人体健康危害,进行生物修复工程,提供了重要的参考依据.     

螺旋藻的应用价值及环境保护功能

本文综述了螺旋藻的基本特性，营养价值，药用价值及开发利用现状，着重讨论了螺旋藻的能源价值和在环境污染净化中的作用，展望了应用发展前景。     

江门市燃生物质成型颗粒锅炉的排污现状与对策研究

生物质成型颗粒是《江门市能源发展"十三五"规划》中着力发展的能源之一.根据江门市燃生物质成型颗粒锅炉当前的使用状况和排污情况,分析总结了造成颗粒物和氮氧化物超标的原因主要为锅炉内部结构、燃烧操作以及燃生物质成型颗粒质量等.在此基础上,探讨改善江门市燃生物质成型颗粒锅炉污染的对策,提出了科学定位生物质成型颗粒,促进行业健康发展,加快各相关企业升级改造,藉此也为其他地区的燃生物质成型颗粒锅炉提供参考.     

Degradation kinetics of terephthalic acid wastewater with the hybrid strains constructed with Phanerochaete chrysosporium

The degradation kinetic parameters in terephthalic acid (TPA) wastewater for the hybrid strains of Fhh and Fhhh obtained through the protoplast fusion of the fungi Phanerochaete chrysosporium (PC) and Saccharomyces cerevisiae Y99, and the native bacteria YZ1 were measured in this research. The highest level of the specific degradation rate for Fhhh and Fhh during 20h reaction were 0.2238 and 0.2163 h^‐1, which were higher than that of their three parental strains and that of the anaerobic bacteria reported. It suggested that the abilities of growth and degradation for Fhh and Fhhh in TPA wastewater were better than their three parental strains. They could create potentials for the purification of TPA wastewater with higher efficiency.     

Chronic exposure to contaminated drinking water stimulates PPAR expression in mice livers

Mice were fed with source water (SW) and tap water (TW) for 90 d to evaluate hepatotoxicity induced by the drinking water. Histopathologic observation showed no obvious damage to hepatic tissue in the SW and TW groups. However, microarray analysis indicated that the SW and TW exposures affected many metabolic pathways, among which PPAR (peroxisome proliferator-activated receptors) signaling was most susceptible. Immunohistochemical staining demonstrated that both PPAR-α and PPAR-γ were significantly increased in the exposure groups compared to control. Enzyme-linked immunosorbent assay revealed that PPAR-α expression level was increased from 23.37±0.53 ng g(-1) liver weight in control group to 26.60±1.43 ng g(-1) liver weight in SW group and 27.68±1.10 ng g(-1) liver weight in TW group (p<0.05). For PPAR-γ, the expression level was also significantly enhanced from 0.83±0.07 ng g(-1) liver weight in control group to 1.11±0.20 ng g(-1) liver weight in SW group and 1.16±0.07 ng g(-1) liver weight in TW group (p<0.05). The SW and DW posed no obvious hepatotoxicity on mice and PPAR-α/-γ could be used as a novel biomarker to assess public health risk induced by slightly contaminated drinking water.