A multi-approach monitoring of particulate matter, metals and PAHs in an urban street canyon

For the first time until now, the results from a prediction model (Atmospheric Dispersion Modelling System (ADMS)-Road) of pollutant dispersion in a street canyon were compared to the results obtained from biomonitors. In particular, the instrumental monitoring of particulate matter (PM10) and the biomonitoring of 14 polycyclic aromatic hydrocarbons (PAHs) and 11 metals by Quercus ilex leaves and Hypnum cupressiforme moss bags, acting as long- and short-term accumulators, respectively, were carried out. For both PAHs and metals, similar bioaccumulation trends were observed, with higher concentrations in biomonitors exposed at the leeward canyon side, affected by primary air vortex. The major pollutant accumulation at the leeward side was also predicted by the ADMS-Road model, on the basis of the prevailing wind direction that determines different exposure of the street canyon sides to pollutants emitted by vehicular traffic. A clear vertical (3, 6 and 9 m) distribution gradient of pollutants was not observed, so that both the model and biomonitoring results suggested that local air turbulences in the street canyon could contribute to uniform pollutant distribution at different heights.     

TAMMEF therapy in the treatment of shoulder periarthritis: efficacy and safety

The utility and the safety of the extremely low frequencies (ELF) electromagnetic fields in the treatment of numerous diseases have been demonstrated. Moreover, the effects of these fields seem to depend on their respective codes (frequency, intensity, waveform). We want to value the effects and the safety of the therapeutic application of a musically modulated electromagnetic field (TAMMEF) system, which field is piloted by a musical signal and its parameters (frequency, intensity, waveform) are modified in time, randomly varying within the respective ranges, so that all possible codes can occur during a single application. Sixty subjects, affected by shoulder periarthritis were enrolled in the study and randomly divided into three groups of 20 patients each: the first exposed to TAMMEF, the second exposed to ELF, the third exposed to a simulated field. All subjects underwent a cycle of 15 daily sessions of 30 min each and a clinical examination upon enrolment, after 7 days of therapy, at the end of the cycle and at a follow-up 30 days later. All the patients of TAMMEF group and ELF group completed the therapy without the appearance of side effects: they presented a significant improvement of the subjective pain and the functional limitation, which remained stable at the follow-up examination. In those exposed to a simulated field group, there was no improvement of the pain symptoms or articular functionality. This study suggests that the TAMMEF system is efficacious and safe in the control of pain symptoms and in the reduction of functional limitation in patients with shoulder periarthritis. Moreover, the effects of the TAMMEF system cover those produced by the ELF field.     

Preliminary studies about effects of ELF and TAMMEF electromagnetic fields on human lymphomonocytes

In our paper we studied the effects of exposure to ELF (extremely low frequency) or musically generated TAMMEF (therapeutic application of musically modulated electromagnetic fields) electromagnetic fields on the tumor necrosis factor alpha (TNF-α) release induced by a lipopolysaccharide (LPS) from cultured human lymphomonocytes of peripheral blood. Lymphomonocytes, isolated from blood donors buffy-coat, were prepared using standard techniques in cell culture flasks, kept in CO₂ incubator, with controlled temperature and humidity for 5 days. Flasks were subjected to an ELF electromagnetic field (100 Hz sinusoidal) or to TAMMEF electromagnetic fields (with intensity, frequency, and wave shape randomly modified in time, so that all possible codes can occur during a single application). The TNF-α release was determined by ELISA test every 24 h for 92 h and the results were evaluated by a non-parametric test. LPS induces a stronger TNF-α release in cultures that were subjected to ELF when compared with cultures subjected to TAMMEF, at each time period of the experimental protocol. These results seem to indicate that TAMMEF is able to induce a complex modulation of LPS-induced TNF-α release, a cytokine with pro inflammatory property whose release during chronic or neoplastic inflammatory diseases has strong negative effects on several organ systems.     

Empirical research in environmental psychology: Past, present, and future

Have research interests in environmental psychology changed over the years? If so, in which direction? What can we learn from the past to direct future research? To answer these questions, empirical studies published in Environment & Behavior (E&B) and in Journal of Environmental Psychology (JEP), from their foundation to 2005, were reviewed. The articles were classified in relation to the following criteria: mode of human–environment transaction, research topic, type of setting and function of places, socio-demographic characteristics and environmental role of people, mode of presentation of the setting, sampling procedure, and source of data. Results showed both variations through the years and differences between the journals. The main research topics can be identified as the study of the residential environment, environmental cognition, observation of actual behaviour in the environment, and concern for the ecological value of the global environment. Trends in research interests showed a stable interest in the analysis of the built environment, a more place-specific approach in the beginning, strongly anchored in observational studies, and a central concern for sustainability and conservation of the environment in recent years. With respect to journals, the central role attributed to psychology by JEP, and the stronger participation by designers and planners in E&B, are reflected in the emphases given to the different modes of human–environment transaction. Trends in research interests help address the strengths and weaknesses of the discipline, thus suggesting future directions of inquiry.     

Temporal variations in PAH concentrations in Quercus ilex L. (holm oak) leaves in an urban area

Temporal variations of polycyclic aromatic hydrocarbon (PAH) concentrations in leaves of a Mediterranean evergreen oak, Quercus ilex L., were investigated in order to assess the suitability of this species to biomonitor PAH air contamination. Leaf samples were collected at six sites of the urban area of Naples (Italy) and at a control site in the Vesuvius National Park, in May and September 2001, and in January and May 2002. PAH extraction was conducted by sonication in dichloromethane-acetone and quantification by GC-MS. In winter, leaf total PAH concentrations showed, at all the urban sites, values 2-fold higher than in all the other samplings, reflecting the temporal trend reported for PAH air contamination in the Naples urban area. Moreover, leaf PAH concentrations showed, at all the urban sites, a decrease in May 2002 after the winter accumulation. At the control site leaf PAH concentrations showed lower values and smaller temporal variations than at the urban sites. The findings support the suitability of Q. ilex leaves to monitor temporal variations in PAH contamination. The highest winter concentrations of total PAHs were due to the medium molecular weight PAHs that increased with respect to both low and high molecular weight PAHs. The medium molecular weight PAHs showed the same temporal trend both at the urban and remote sites.     

Biological evidences than human osteoarthritic chondrocytes could be affected by electromagnetic fields with extremely low-frequency or new TAMMEF system

Osteoarthritis (OA) is a chronic degenerative condition involving the joints caused by metabolic changes in chondrocytes metabolism. Treatment with magnetic fields (EMFs) produces benefits to patients affected by this pathology. The present research has the aim to investigate, by a proteomic approach, the effect of magnetic fields on cultured chondrocytes, because EMFs interferes greatly with OA chondrocytes metabolism. Human osteoarthritic chondrocytes were obtained from the femur head of osteoarthritis adult patients and were cultured under standard conditions and exposed for precise times both to extremely low-frequency (ELF; 100-Hz) electromagnetic fields and to the Therapeutic Application of Musically Modulated Electromagnetic Fields (TAMMEF), which are characterized by variable frequencies, intensities, and wave shapes. We tested the effects of the different exposure by 2-DE analysis and demonstrated that chondrocytes metabolism was highly influenced by the treatment with EMFs; in fact many proteins change their expression after the stimulation, but differently, depending on whether we use ELF or TAMMEF system.     

Direct and indirect photolysis of cyhalofop in aqueous systems

The photodegradation of the aryloxyphenoxy propionic herbicide cyhalofop-butyl (2R)-2-[4-(4-cyano-2-fluorophenoxy)phenoxy]butylpropanoate (CyB), and of its primary metabolite (2R)-2-[4-(4-cyano-2-fluorophenoxy)phenoxy]propanoic acid (CyA) was studied in water at different irradiation wavelengths. The sunlight irradiation was investigated also in the presence of humic acid (HA), Fe oxide, titanium dioxide (TiO₂) and zinc oxide (ZnO) as photocatalysts.CyB and CyA were rapidly degraded by UV irradiation. CyB afforded the butyl ester of 2-[3-(4-cyano-2-fluorophenyl)-4-hydroxy-phenoxy]propanoic acid (CyI), a metabolite arising from a photo-Fries rearrangement. Instead, CyA yielded (R)-2-4-(4-carboxyl-2-fluorophenoxy)phenoxypropanoic acid (CyD), a dicarboxylic acid arising from the photo-hydrolysis of cyano group via amide. CyB was stable in simulated sunlight also in the presence of the catalysts tested.The irradiation of a CyA solution, in the presence of HA or Fe oxide, with simulated sunlight did not produce any significant degradation. In the same experimental conditions, CyA was totally mineralized in the presence of TiO₂ and ZnO.     

Lysosomal,genetic and chromosomal damage in haemocytes of the freshwater bivalve (Unio pictorum) exposed to polluted sediments from the River Cecina (Italy)

The response to chemical disturbance of an Italian river (Cecina River) characterised by high levels of trace metals was assessed in the sentinel species freshwater painter’s mussel (Unio pictorum). We exposed U. pictorum in the laboratory to metal, metalloid and other trace element polluted river sediments in order to evaluate genetic and cellular biomarkers. Bivalves exposed to sediments taken from all the impacted sites of the river basin exhibited a significant impairment at the DNA, chromosomal and lysosomal levels. The lysosomal membrane stability of circulating haemocytes, assessed by the Neutral Red Retention Time, was found to be higher in specimens from Lake Maggiore and Berignone, while exhibited a significant decrease in specimens exposed to Ponteginori sediments. An increase of DNA migration in specimens exposed to the two contaminated sites in comparison with controls was found. Moreover, higher micronucleated haemocytes frequencies were observed in bivalves exposed to sediments collected at Possera 1 site. This study suggested that biomarkers evaluating cellular impairment, genetic and chromosomal damages, are highly sensitive and suitably applied to laboratory experiments. The painter’s mussel was confirmed to be an appropriate sentinel species for quality assessment in metal-contaminated freshwater environments.     

Efficacy and safety of new TAMMEF (therapeutic application of musically modulated electromagnetic fields) system in the treatment of chronic low back pain

Numerous studies have demonstrated the utility of extremely low frequencies (ELF) electromagnetic fields in the treatment of pain. Moreover, the effects of these fields seems to depend on their respective codes. In our study we want to value the effects and the safety of the TAMMEF (Therapeutic Application of a Musically Modulated Electromagnetic Field) system, which field is piloted by a musical signal and its parameters are modified in time, randomly varying within the respective ranges, so that all possible codes can occur during a single application. Ninety subjects, affected by chronic low back pain, were enrolled in the study and randomly divided into three groups of 30 patients each: A exposed to TAMMEF, B exposed to ELF, C exposed to a simulated field. All subjects underwent a cycle of 15 daily sessions of 30 minutes each and a clinical examination upon enrolment, after 7 days of therapy, at the end of the cycle and at a follow-up 30 days later. All the patients of groups A and B completed the therapy without the appearance of side effects: they presented a significant improvement of the subjective pain and the functional limitation, which remained stable at the follow-up examination. In group C, there was no improvement of the pain symptoms or articular functionality. This study suggests that the TAMMEF system is efficacious and safe in the treatment of patients with chronic low back pain.