The aim of this study was to survey the response of the microbial community to crude oil and the diversity of alkane hydroxylase (alkB) genes in soil samples from the Qinghai-Tibet Plateau (QTP). The enrichment cultures and clone libraries were used. Finally, 53 isolates and 94 alkB sequences were obtained from 10 pristine soil samples after enrichment at 10 °C with crude oil as sole carbon source. The isolates fell into the phyla Proteobacteria, Actinobacteria, and Bacteroidetes, with the dominance of Pseudomonas and Acinetobacter. The composition of degraders was different from polar habitats where Acinetobacter sp. is not a predominant responder of alkane degradative microbial communities. Phylogenetic analysis showed that the alkB genes from isolates and enrichment communities formed eight clusters and mainly related with alkB genes of Pseudomonas, Rhodococcus, and Acinetobacter. The alkB gene diversity in the QTP was lower than marine environments and polar soil samples. In particular, a total of 10 isolates exhibiting vigorous growth with crude oil could detect no crude oil degradation-related gene sequences, such as alkB, P450, almA, ndoB, and xylE genes. The Shannon-Wiener index of the alkB clone libraries from the QTP ranged from 1.00 to 2.24 which is similar with polar pristine soil samples but lower than that of contaminated soils. These results indicated that the Pseudomonas, Acinetobacter, and Rhodococcus genera are the candidate for in situ bioremediation, and the environment of QTP may be still relatively uncontaminated by crude oil. 相似文献
Environmental Science and Pollution Research - There is profound interest in knowing the degree to which the effectiveness of China’s nature reserves, and whether leakage is common around the... 相似文献
Ecological indicators have widespread appeal to scientists, environmental managers, and the general public. Remote sensing is unique in its capability to record variety of spatio-temporal information on land surface with complete coverage, especially with regard to larger spatial scales, which has been proven to be an effective data source to create indicators to rapidly identify regional eco-environment. In this paper, a new index, remote sensing based ecological index (RSEI) based on the pressure-state-response (PSR) framework, was applied to assess regional ecological changes in Fuzhou City of Fujian Province, southeastern China, using Landsat ETM+/OLI/TIRS images. Taking the advantages of being totally free of artificial interference in the calculation using principal components analysis (PCA) to assign weights of each variable, the RSEI can assess the regional ecological status more objectively and easily. The effectivity of the new index was validated by four approaches, including point-based, classification-based, correlation-based, and urban-rural-gradient-based comparisons. The case study showed that Fuzhou has witnessed ecological improvement during the study period, with the value of RSEI increasing from 0.663 in 2000 to 0.675 in 2016. Spatial variation analysis showed that the poor level of RSEI distributed mostly in the central urban areas, and the ecological degradation was attributed to the fast expansion of the built-up area, characterized by increasing greatly in the value of the normalized differential built up and soil index (NDBSI) in such areas.
Hydroxyapatite (HAP) has been widely used to immobilize many cationic metals in water and soils. The specific reason why an increase in the surface area of HAP enhances cadmium (Cd) uptake, but has no effect on lead (Pb) uptake, is not clear. The aim of this study was to determine the factors causing the differences in sorption behavior between Cd and Pb by evaluating HAPs with different surface areas. We synthesized HAPs with two different surface areas, which were characterized by X-ray diffraction, N2 adsorption, and scanning electron microscopy, and then evaluated them as sorbents for Cd and Pb removal by testing in single and binary systems. The sorption capacity of large surface area HAP (1.85 mmol/g) for Cd in the single-metal system was higher than that of small surface area HAP (0.64 mmol/g), but there were no differences between single- and binary-metal solutions containing Pb. After the Cd experiments, the HAP retained a stable structure and intact morphology, which promotes the accessibility of reactive sites for Cd. However, a newly formed precipitate covered the surface and blocked the channels in the presence of Pb, which reduced the number of potential adsorption sites on HAP for Cd and Pb. Remediation experiments using Cd- and Pb-contaminated soil produced similar results to the solution tests. These results indicate that alterations of the structure and morphology during the reaction is an important factor influencing metal sorption to HAP. 相似文献