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介绍了蔗糖厂自愿清洁生产审核中的审核重点、审核目标的确定,清洁生产方案的产生和筛选,以及清洁生产审核效果的统计。 相似文献
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介绍了蔗糖厂自愿清洁生产审核中的审核重点、审核目标的确定,清洁生产方案的产生和筛选,以及清洁生产审核效果的统计。 相似文献
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Hans Preusting Jaap Kingma Gjalt Huisman Alexander Steinbüchel Bernard Witholt 《Journal of Polymers and the Environment》1993,1(1):11-21
WhenPseudomonas oleovorans (GPo1) is grown on sodium octanoate under ammonium limiting conditions, it is able to accumulate a copolyester consisting of medium chain length 3-hydroxyalkanoic acids (PHAm). 3-Hydroxybutyrate is only incorporated in trace amounts. WhenP. oleovorans is equipped with the PHB biosynthetic genes ofAlcaligenes eutrophus (GPo1[pVK101::PP1]), it forms a polyester containing major amounts of 3-hydroxybutyrate. The resulting polymer however is a blend of PHAm and PHB, rather than a copolymer of 3-hydroxybutyrate and medium chain length 3-hydroxyalkanoic acids [11]. To establish whether PHAm and PHB molecules are stored in the same or separate granules by this recombinantP. oleovorans strain, we studied polymer forming cells by freeze-fracture electron microscopy. This approach is possible because previous freeze-fracture electron microscopy studies on PHAm and PHB accumulating strains have shown that PHAm and PHB granules can be distinguished from each other: PHAm granules from mushroom-like structures, whereas PHB granules from needle structures during freeze-fracturing. In this paper we show that stationary phase cells of GPo1[pVK101::PP1] contained both mushroom and needle-like structures, indicating that PHAm and PHB chains were stored in separate granules. To be able to determine whether the separation of PHAm and PHB is complete, the respective granules were separated on sucrose gradients. A total cell extract of GPo1[pVK101::PP1] which was subjected to sucrose gradient centrifugation revealed two white bands of different densities: the upper band with a density of 1.05 g/mL consisted exclusively of PHAm granules, while the lower band with a density of 1.19 g/mL consisted of PHB granules only. Thus, when bacteria synthesize both PHAm and PHB, the resulting polymer chains are segregated completely and stored in separate granules. 相似文献
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姚蓉 《应用与环境生物学报》1996,(1)
用一组多克隆抗体对马氏甲烷八叠球菌(Methanosarcinamazei)S-6菌株的基因组DNA文库进行了筛选.仅选出p60A克隆能对马氏甲烷八叠球菌中可发生细胞形态学变化菌株的抗血清发生阳性反应.对p60A克隆的双链DNA进行了序列分析.识别出一开式阅读框架(openreadingframeP,ORFP).表达的蛋白是ORFP编码的蛋白的3倍,并得到ORFP蛋白的三聚体,在SDS-PAGE中表现出整体蛋白的迁移行为.同时,此表达蛋白抗10%SDS,6mol/L尿素及热处理.基因结构分析表明,ORFP具有与M.barkrimcrA有同源性的核糖体结合位点和一个与甲烷细菌启动子共有序列相似度达77%的启动子序列.使用参照序列分析表明,由ORFP演绎的氨基酸序列,具有高密度的带电荷的氨基酸和占优势的β-层迭构型.对此表达蛋白作了Neurosroracrassa的porin蛋白抗血清试验,以研究其可能功能.检验了M.mazeiS-6细胞的蔗糖梯度制备物,对此原细胞中的ORFP蛋白作了定位.结果表明,ORFP蛋白可能是一种古细菌Porin,其在M.mazeiS-6中的表达,可能象大肠杆菌那样与渗透压调节有关. 相似文献
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C. H. Schilling S. B. Biner H. Goel J. Jane 《Journal of Polymers and the Environment》1995,3(3):153-160
Traditional methods for the shape-forming of engineering ceramics entail plastic deformation of powder slurries containing hazardous organic liquids as suspending media. Replacing these organic with aqueous media requires the development of environmentally benign, water-soluble additives which serve as plasticizers and binders. Fundamental studies were performed with aqueous suspensions of colloidal-Al2O3 to evaluate the role of sucrose and maltodextrin on viscosity, sedimentation, and filtration characteristics, plastic flow behavior of filter cakes, and sinterability. Maltodextrin systems exhibited superior results, including filtration to high packing densities and clay-like plasticity with minimal cracking.Paper presented at the Bio/Environmentally Degradable Polymer Society—Third National Meeting, June 6–8, 1994, Boston, Massachusetts.Ames Laboratory is operated for the U.S. Department of Energy by Iowa State University under Contract W-7405-Eng-82. 相似文献
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根肿(Plasmodiophora brassicae)休眠孢子的纯化和超声波破碎方法研究 总被引:2,自引:0,他引:2
根肿(Plasmodiophora brassicae Woronin)是严重危害十字花科植物的世界性病害.采用蔗糖密度梯度离心和超声波处理方法从油菜染病根部组织分离和提纯根肿休眠孢子.众多因素可以影响提纯休眠孢子的纯度,为了得到更高纯度和均匀度的休眠孢子的优化方法,我们对一系列影响因素如超声波破碎功率、pH、孢子破碎浓度、处理时间和不同的水溶物质等进行了研究.在pH值5.5左右、添加0.5%的二甲基亚砜、孢子浓度约为7×108、345W功率下超声波处理10min条件下获得了最优数量和均匀度的孢子,杂菌数量减少了51%以上,杂菌种类减少超过75%.超声波处理50min后,孢子破碎率稳定地达到90%以上,除菌率达99%以上.用此方法能在较短时间内获得高纯度和高均匀度的休眠孢子,为休眠孢子的深入研究提供了资料.图4表3参10 相似文献
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Summary Changes in the density of beech scale infestation (Cryptococcus fagisuga) of a mature beech stand (Fagus sylvatica) were investigated after gap-cutting in limed and unlimed areas bordering on the gaps and in untreated areas over a 5-year period. Parallelly the concentrations of sucrose, protein amino acids, and procyanidins were examined in the inner and outer bark of non-infested beech trees and beeches infested by beech scale.Irrespective of liming in the areas bordering on the gaps the proportion of beech trees with increasing beech scale infestation was significantly higher, the proportion of beeches with diminishing infestation was significantly lower than in the untreated control areas. Trees with the same infestation tendency occurred in clusters.Physiological defence reactions in the inner and outer bark of the infested beech trees were registrated as an increase in the procyanidin content and a decrease in the protein amino acid content. A change in the outer bark—inner bark—ratio of the infested beech trees showed a transfer of compounds between the inner and outer bark. During the period of observation long-lasting shifts occurred in the pattern of compounds of the bark irrespective of actual infestation intensity. 相似文献
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崇左市是全国最大的蔗糖生产和加工基地,被誉为“中国糖都”,甘蔗种植水平和制糖企业的生产技术水平位于广西前列,蔗糖循环经济初具规模.对崇左市发展蔗糖循环经济的基本条件、目标原则及发展途径进行系统分析,为政府加快发展蔗糖循环经济提出对策建议. 相似文献