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Enzymatic decolourization of the azo dye, Direct Yellow (DY106) by Cucurbita pepo (courgette) peroxidase (CP) is a complex process, which is greatly affected by pH, temperature, enzyme activity and the concentrations of H2O2 and dye. Courgette peroxidase was extracted and its performance was evaluated by using the free-CP (FCP) and immobilized-CP (ICP) forms in the decolourization of DY106. Immobilization of peroxidase in calcium alginate beads was performed according to a strategy aiming to minimize enzyme leakage and keep its activity at a maximum value by optimizing sodium alginate content, enzyme loading and calcium chloride concentration. The initial conditions at which the highest DY106 decolourization yield was obtained were found at pH 2, temperature 20℃, H2O2 dose 1 mmol/L (FCP) and 100 mmol/L (ICP). The highest decolourization rates were obtained for dye concentrations 50 mg/L (FCP) and 80 mg/L (ICP). Under optimal conditions, the FCP was able to decolorize more than 87% of the dye within 2 min. While with ICP, the decolourization yield was 75% within 15 min. The decolourization and removal of DY106 was proved by UV-Vis analysis. Fourier transform infrared (FT-IR) spectroscopy analysis was also performed on DY106 and enzymatic treatment precipitated byproduct. 相似文献
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众所周之,安全生产工作是一项复杂的系统工程,需要包括政府和企业在内的全社会共同努力:而其中的“两个主体”(政府监管主体和企业责任主体)和“两个负责制”(行政首长负责制和企业法人代表负责制)显得尤为重要,然而在政府主管部门三令五申下,仍有企业“鸟枪充炮”,对安全生产应付了事。 相似文献
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中秋、国庆节长假期间,一些高速路变成拥堵不堪的"龟速路",景区变成"寸步难行"的人海,假日休闲变成"花钱买罪受"。随之而来的也产生了一些不环保的行为。今年中秋、国庆8天长假期间,在全国首次实行重大节假日收费公路免费和174家景区门票降价利好政策的刺激下,民众出行呈现出"井喷"之势。与旅游相关的吃、住、行、购物等消费快速增长,提振了假日经济。但随之产生的多地高速公路拥堵,造成汽车废气排放增加、人们在高速公路和景点乱丢垃圾等不环保的行为,困扰着 相似文献
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综合实践活动课程是一个与各学科课程既有密切联系、又有着本质区别的新领域。然而,如何在实际教学中开展实践活动却是一个难题。鉴于此,我校以采蜜园为基地,以花卉种植为专题,在开发综合实践活动课程方面进行了一些尝试,并收到了良好的效果。 相似文献
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一次性牙刷、牙膏、香皂、浴液、拖鞋、梳子等六种酒店免费提供的日常用品被称为酒店“六小件”。长期以来,下榻酒店的顾客习惯于享受“六小件”带来的便利。但不久后,这些免费提供的一次性消费品或许在广东将受禁止,免费提供将成为历史。 相似文献
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1992年,在我刚刚担任县环保局办公室主任的时候,就认识了《环境工作通讯》,作为一套免费赠送的内部资料,我力争每期必读,并从中了解和学习了一些国家环保政策和时事新闻、大事记,从而也提高了自己的环保法制理论水平。 相似文献