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现代生物技术发展虽历时短暂,但它为解决人类生存和发展危机,实现可持续发展战略带来了一线曙光,因而倍受世人瞩目.同时,由于现代生物技术存在着广泛、潜在而长期的危害及发展不平衡性,从而给人类赖以生存与发展的环境构成了巨大威胁和严峻挑战;它既危及到人类的环境安全,又削弱了可持续发展基础.笔者在分析现代生物技术对人类生存和发展环境构成的威胁与挑战的基础上,探讨了中国入世后所面临的更加严峻的环境安全与压力,并提出规范现代生物技术,促进可持续发展的对策. 相似文献
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盐藻烯醇酶基因表达载体的构建及其转基因烟草的鉴定 总被引:2,自引:1,他引:2
以载体pCAMBIA2301为骨架引入pBI121中的GUS基因,构建了简便、实用的中间载体pCAMBIA2301G.将其中一个GUS基因用目的片段盐藻烯醇酶基因替换,构建了可以在植物中高效表达的载体pCAMBIA2301G—enolase,并将其转入根癌农杆菌EHA105中,采用叶盘转化法将盐藻烯醇酶基因转入模式植物烟草中.Southern杂交结果显示,盐藻烯醇酶基因已整合到植物基因组中,在转基因烟草中的拷贝数为2~4个.图6参10 相似文献
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以质粒pCUBAC-HPT作抗虫基因供体,优良光温敏核不育系612S为受体,采用基因枪转化法,获得了转抗虫基因sck和sbk的植株.分子证据表明,外源基因己整合到受体基因组中.蛋白活性测定和Western blotting结果显示,转基因在受体植株中得到表达.田间抗虫性测定表明,外源基因的转入提高了受体的抗虫性.本文还讨论了转基因技术在水稻育种中的作用.图5表2参12 相似文献
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Sean?A.?WeaverEmail author Michael?C.?Morris 《Journal of Agricultural and Environmental Ethics》2005,18(2):157-189
We present an annotated bibliography of peer reviewed scientific research highlighting the human health, animal welfare, and environmental risks associated with genetic modification. Risks associated with the expression of the transgenic material include concerns over resistance and non-target effects of crops expressing Bt toxins, consequences of herbicide use associated with genetically modified herbicide-tolerant plants, and transfer of gene expression from genetically modified crops through vertical and horizontal gene transfer. These risks are not connected to the technique of genetic modification as such, but would be present for any conventionally produced crops with the same heritable traits. In contrast, other risks are a direct consequence of the method used in gene manipulation. These come about because of the unstable nature of the transgene and vectors used to insert it, and because of unpredictable interactions between the transgene and the host genome. The debate over the release of genetically modified organisms is not merely a scientific one; it encompasses economics, law, ethics, and policy. Any discussion on these levels does, however, need to be informed by sound science. We hope that the scientific references provided here will provide a useful starting point for further debate. 相似文献
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