氟化钠与强致突变物联合诱致的高等植物细胞微核效应

氟化钠在与强致突变物的联合实验中均发生较强的颉颃(减毒)作用,例如非常显著地抑制由平阳霉素(染色体断裂剂)或秋水仙素(有丝分裂毒素)诱致的微核效应(F＇相似文献   

Chromosome doubling of Sedum alfredii Hance: A novel approach for improving phytoremediation efficiency

Sedum alfredii Hance is a cadmium(Cd)/zinc(Zn) hyperaccumulator native to China.However, its relatively low biomass restricted the large-scale application for heavy metal contamination remediation. The chromosome set doubling of S. alfredii in vitro was achieved by 0.1%–0.2%(W/V) colchicine treatment. The plant DNA ploidy was analyzed by flow cytometry and chromosome set doubling plants(CSD) were identified based on the obvious different sharp peak. A tissue culture experiment with different Cd treated levels and a field trial with natural polluted mined soil were conducted to study the effects of chromosome doubling on plant biomass and Cd accumulation in shoots. The results suggested that S.alfredii is a mixoploid. Compared with the wild type plants(WT), CSD exhibited typical"gigas" characteristics in morphology including stem thickness, root hair production,number of leaves and size of stoma guard cell. Fresh weight and dry weight of CSD were increased to 1.62–2.03-fold and 2.26–3.25-fold of WT. And Cd content of CSD showed a17.49%–42.82% increase and 59% increase under tissue culture and field condition,accordingly. In addition, the TF and in BCF of CSD were 2.37-and 1.59-fold of WT,respectively. These results proved that it is feasible to promote phytoextraction efficiency of S. alfredii in Cd contaminated soils through chromosomal engineering, which provides a novel approach for hyperaccumulator application in phytoremediation.