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排序方式: 共有390条查询结果,搜索用时 15 毫秒
1.
Dr. J. F. Bruch P. Metezeau N. Garcia-Fonknechten Y. Richard V. Tricottet B.-L. Hsi A. Kitzis C. Julien E. Papiernik 《黑龙江环境通报》1991,11(10):787-798
Three monoclonal antibodies (MAbs) against trophoblast (GB17, GB21, and GB25) and flow cytometry were used to sort trophoblast-like cells (TLCs) from peripheral blood of pregnant women. Sorted TLCs were processed for electron microscopy and fetal DNA amplification of the Y-specific sequences from mothers carrying male fetuses. At the ultra-structural level, most of the nucleated cells had the morphology of leucocytes, suggesting maternal contaminants, and we did not find the characteristic features of the free inter-villous trophoblast cells. Nevertheless, polymerase chain reaction (PCR) analysis showed an amplification of Y-specific sequences in two out of three samples of sorted TLCs. These results suggest that besides the maternal leucocytes, sufficient trophoblast nucleated fetal cells can be obtained using cell enrichment by sorting. This sensitive method holds promise for non-invasive prenatal diagnosis of fetal sex and if sufficient Y(positive) nuclei are found, for the diagnosis of selected numerical chromosome abnormalities. 相似文献
2.
南宁城市大气污染对人体健康的危害及治理对策 总被引:6,自引:0,他引:6
南宁市大气属煤烟型污染 ,大气的主要污染物为SO2 、NOx、TSP、降尘 ,虽然随着环境管理和污染治理工作的加强 ,污染物浓度逐年下降 ,但是工业区大气污染仍然较重。污染物流行病学调查显示 :工业区癌症和呼吸系统疾病死亡率均高于全市平均水平 2倍左右 ,城区又高于郊区 2倍 ,大气污染综合指数与呼吸内科门诊就诊人数呈正相关。用邓聚龙的灰色系统理论分析得知污染物对癌症和呼吸系统疾病死亡率有关联 ,关联度从大到小排序为 :TSP >降尘 >SO2 >NOx ,最后提出了大气污染治理的对策。 相似文献
3.
中国乳腺癌与土壤环境中化学元素的相关性研究 总被引:12,自引:1,他引:12
利用土壤元素资料2069448个数据、乳腺癌死亡调查资料31320例,研究了乳腺癌死亡率与人群生存的土壤环境中64个元素的相关性。结果表明,砷、汞、钡、铊、钕、铀、锆、锑等元素与乳腺癌死亡率有相关性。等级相关系数分别为-0.6820(P〈0.0005)、0.4567(P〈0.01)、-0.3773(P〈0.025)、-0.3852(P〈0.025)、0.3179(P〈0.05)、-0.4377(P〈 相似文献
4.
5.
Dr Alan E. Donnenfeld Larry R. Glazerman Denise M. Cutillo Ronald J. Librizzi Stuart Weiner 《黑龙江环境通报》1989,9(5):301-308
Selective termination by intracardiac potassium chloride injection was performed in twins discordant for hydrocephaly at 20 weeks' gestation. Because of the potential for vascular anastomoses to exist between the twins, fetal angiography was performed prior to the selective termination procedure. Determination of vascular connections between the fetuses was hindered by fetal bradycardia following intracardiac administration of contrast material. Selective termination was performed without difficulty using intracardiac potassium chloride (KCl) to produce asystole in the twin with hydrocephaly. The unaffected fetus appeared active and had a normal heart rate during and immediately after the procedure. However, both twins were found to have died the following day. Pathologic examination documented several vascular anastomoses between the monochorionic, diamniotic fetuses. A likely cause of death was exsanguination of the normal twin into the abnormal one. This case illustrates the difficulties encountered in selective termination of monozygotic twins and, to our knowledge, represents the first reported use of intrauterine fetal angiography. 相似文献
6.
Richard J. Stock M.D. 《黑龙江环境通报》1982,2(2):133-137
A case of fetal death secondary to a needle laceration during a second trimester amniocentesis is presented. The need for careful pathologic evaluation of fetuses lost subsequent to these procedures is stressed. 相似文献
7.
Yuen Tannirandorn Umberto Nicolini Peter C. Nicolaidis Nicholas M. Fisk Sabaratnam Arulkumaran Charles H. Rodeck 《黑龙江环境通报》1990,10(3):189-193
Twelve second-trimester fetuses with cystic hygroma underwent fetal blood sampling for rapid karyotyping, haematologic evaluation, and blood gas analysis. An abnormal karyotype was found in seven cases: monosomy X in five, trisomy 21 in one, and trisomy 13 in the other. Eight often fetuses undergoing blood gas analysis showed hypoxaemia, five of which were growth-retarded. Nine pregnancies were terminated. Of the remaining three, only one fetus survived the perinatal period. 相似文献
8.
Emanuela Terzian Jillian Boreham Howard S. Cuckle Nicholas J. Wald Martin Bobrow Richard Lindenbaum A. C. Turnbull 《黑龙江环境通报》1985,5(6):401-414
A survey was conducted of the results of mid-trimester diagnostic amniocenteses in the Oxford Region from 1974 to 1981. The survey used data relating to all 4357 singleton pregnancies in which an amniocentesis was performed during this period. Follow-up information on outcome was obtained in respect of 4284 (98 per cent) pregnancies. A cell culture to determine karyotype and an alpha-fetoprotein determination was carried out in all cases. From 1974 to 1981 amniocenteses became increasingly common, rising from 2 to 32 per 1000 births. The most common indication for amniocentesis was a high risk of a chromosome abnormality–56 per cent of all amniocenteses. Within this group advanced maternal age was responsible for 89 per cent of the cases. The next most common indication was a high risk of a neural tube defect (37 per cent of all amniocenteses)–in 1974 a raised maternal serum alpha-fetoprotein level accounted for only 4 per cent of these; by 1981 this had risen to 67 per cent. There were seven false-positive and 132 true-positive diagnoses of neural tube defect; since 1981, with the introduction of amniotic fluid acetylocholinesterase determination as a secondary diagnostic test for neural tube defects, there have been no further false-positive diagnoses. In 1981 76 per cent of women aged 35 years or more did not have an amniocentesis. It is not known to what extent this was due to not offering women in this age group amniocentesis or to women not accepting such an offer. 相似文献
9.
Hurler's disease was excluded in a fetus at 23 weeks' gestation by demonstrating normal iduronidase activity in fetal leucocytes following failure of amniotic cell culture after amnic-centesis at 16 and 19 weeks' gestation. The diagnosis was confirmed in the neonate. 相似文献
10.
We report on a case of trisomy 8 mosaicism detected prenatally in a single clone of amniotic fluid culture, and confirmed on fetal blood and on peripheral lymphocytes after birth. A follow-up was performed over 3 years, showing a clinically normal female with cognitive, neuropsychological, and linguistic development in a normal range. 相似文献