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Silvana Vanucci 《Chemistry and Ecology》2003,19(4):299-320
Temporal changes in abundance and biomass of picophytoplankton, heterotrophic pico-eukaryotes, and nanoplankton assemblages were investigated along a transect crossing the Adriatic Sea, from the Italian to the Croatian coast. This 15-months (June 1999-August 2000) investigation allowed comparing microbial parameters during summer 1999 (year without mucilage) and summer 2000 when a major mucilage event occurred. Pico- and nanoplankton assemblages displayed significant differences between the 2 summer periods. The main differences can be summarized as: (i) presence of cyanobacterial blooms (up to 108 cells l-1) in summer 2000, absent in summer 1999; (ii) an increasing fraction of heterotrophic pico-eukaryotes (up to 5.0 × 106 cells l-1) and heterotrophic nanoplankton (size 2-5 µm) during mucilage event; (iii) a reduced abundance of small-sized (2-3 µm) phototrophic nanoplankton in summer 2000. Changes in community structure were signals of changes in trophic condition of the system, which resulted in a competitive advantage for small sized pico- and nanoheterotrophs. Data presented here indicated that mucilage events are associated with changes in microbial community structure and functioning in ambient water and induced the amplification of 3-step microbial food chain. The potential use of the heterotrophic pico-eukaryotes for describing alterations of the trophic pathways during mucilage events is discussed. 相似文献
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新型异养氨氧化菌的分离鉴定及氨氧化特性 总被引:1,自引:0,他引:1
从青岛海岸采集淤泥.通过富集培养,以硅胶平板点种分离.经纯化得到一株氨氧化菌H9菌株。该菌可以在营养培养基中生长,是一株异养氨氧化菌,经形态、生理生化特性、16SrRNA序列分析等初步鉴定该菌属于假诺卡氏菌属(Pseudonocardia sp.H9)。该菌株具有将氨氧化为硝酸的能力,明显不同于自养型的硝化作用过程需要亚硝化细菌和硝化细菌的密切协作才能把氨氧化成硝酸的特点。此外,该菌可以在无有机碳的培养基或在有机碳C/N达7或更高的培养基中氧化氨至硝酸;在亚硝化培养基与LB的混合培养基中,在LB占15%以下时,LB越高越有利于氨的氧化和菌的生长。该菌株的生长和氧化氨的最适温度为30℃;pH5.0~10.0,最适为8.0;氨离子摩尔浓度1~40mmol/L,最适为8mmol/L;氯化钠质量分数为0%~8%,最适为3.5%。由于该菌株具有以上的特性和优点.在污水的脱氮处理以及养殖海水的氨氮处理中有较高的应用前景,同时也是研究氨的生物氧化机制的理想菌株。 相似文献
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异养硝化及其在污水脱氮中的作用 总被引:23,自引:0,他引:23
通过与传统自养硝化作用的比较,异养硝化作用不仅是客观存在的过程,而且某些特殊的异养菌,可以同步进行异养硝化和好氧反硝化,对于污水脱氮具有重要的理论意义和应用价值。 相似文献
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J. Bai K. R. Li D. Y. Liu J. Zhang H. F. Zhang G. S. Liu J. L. Li Z. Y. Li D. M. Gao 《Water, Air, & Soil Pollution: Focus》2007,7(6):673-681
The distribution patterns and factors affecting uptake rates and turnover times of dissolved inorganic nutrients by heterotrophic
bacterioplankton (HB) in Jiaozhou Bay were studied based on in situ surveys and laboratory culturing. The uptake rates of
dissolved inorganic phosphorus (DIP) and dissolved inorganic nitrogen (DIN) by HB are limited by the temperature of the water
and the concentration of dissolved organic carbon. The turnover times of DIP and DIN by HB are regulated by the concentrations
of the nutrients and the numbers of HB, which show significant spatiotemporal variability in Jiaozhou Bay. HB may aggravate
the P-limitation of phytoplankton by competing for phosphorus in summer in Jiaozhou Bay. HB play an important role in the
biogeochemical cycling of nutrients and may increase the N:P ratio and the accumulation of DIN, thereby accelerating the imbalance
of the N:P ratio in Jiaozhou Bay. 相似文献
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Water biostability is of particular concern to water supply as a major limiting factor for heterotrophic bacterial growth in water distribution systems. This study focused on bacterial growth dynamics in the series dilution of water samples with TOC(total organic carbon) values determined beforehand. The results showed that the specific growth rate of Pseudomonas fluorescens P17 varied dramatically and irregularly with TOC value when TOC concentrations were low enough during the initial periods of incubation under given conditions. According to this relationship between bacterial growth rate and TOC, a dilution incubation method was designed for the study of water biostability. With the method under a given condition, a turning-point TOC value was found at a relatively fixed point in the curve between bacterial growth rate and TOC of water sample, and the variation of growth rate had different characteristics below the turning-point TOC value relative to that over this value. A turning-point TOC value similarly existed in all experiments not only with tap water, but also with acetate and mixed solutions. And in the dilution incubation method study, the affections were analyzed by condition factors such as inoculum amount,incubation time and nature of the organic carbon source. In very low organic carbon water environments, the variation characteristics of bacterial growth rate will be useful to further understand the meaning of water biostability. 相似文献
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Jiajun Hu Lei Wang Shiping Zhang Yuanqing Wang Fangming Jin Xiaohua Fu Huirong Li 《环境科学学报(英文版)》2014,26(8):1709-1716
The universality of improved CO2 fixing upon the addition of mixed electron donors(MEDs)composed of Na2 S,NO2-,and S2O32-to non-photosynthetic microbial communities(NPMCs)obtained from 12 locations in four oceans of the world was validated. The CO2 fixing efficiencies of NPMCs were universally enhanced by MED compared with those obtained using H2 alone as electron donor,with average increase of about 276%. An increase in microbial inoculation concentration could increase the net amount of CO2 fixing to853.34 mg/L in the presence of MED. NO2-and S2O32-may play the roles of both electron acceptor and electron donor under aerobic conditions,which may improve the energy utilization efficiency of NPMC and enhance the CO2 fixation efficiency. The sequence determination of 16 S ribosomal deoxyribonucleic acid(rDNA) from 150 bacteria of NPMC showed that more than 50% of the bacteria were symbiotic and there were many heterotrophic bacteria such as Vibrio natriegens. These results indicate that NPMC acts as a symbiotic CO2 fixing system. The interaction between autotrophic and heterotrophic bacteria may be a crucial factor supporting ladder utilization and recycling of energy/carbon source. 相似文献