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水体中隐孢子虫卵囊的检出及其活力分析   总被引:2,自引:0,他引:2  
从水中检测隐孢子虫卵囊是评价水源传播人类隐孢子虫病风险的第一步,从水中检出的卵囊的活力需要进行测定才能估计水源爆发的真正风险,应用聚丙烯盒式过滤器和美国环保局(USEPA)1622过滤方法的囊式过滤器(Gelman,Michigan,USA),分别过滤了当地(法国鲁昂)的环境水和供给水,并用免疫磁分离技术(IMS)进行分离。用免疫荧光检测法(IFA)从浓缩的水样中检测到了隐孢子虫卵囊。环境水中的卵囊浓度是每升水中0.12-2.7个卵囊;供给水中的卵囊浓度是每升水中0.03-0.06个卵囊,用新生小鼠模型初步评价了检出卵囊的感染力。结果证明了隐孢子虫卵囊在环境中尤其是水中的广泛分布;同时表明,评估水源传播的隐孢子虫病的风险时,不仅要从水中检出卵囊,而且要测定检出的卵囊的活力。表1参22。  相似文献   
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Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia under USEPA Method 1623. In this study, IMS was evaluated on the basis of recovery efficiencies for Giardia cysts under various IMS operation conditions. Significant change in recovery was observed by altering the debris ratio of water samples. Notably, cyst recovery efficiencies utilizing IMS dropped with increased turbidity, and results for varying dosages of magnetic beads and cysts indicate that 1 / 100 immunomagnetic beads is sufficient to conjugate large numbers of cysts. Changing vessel volume and replacing the sample buffer had no significant effect on cyst recovery efficiencies.  相似文献   
3.
采用澳大利亚源流域水量水质模型模拟东山小流域内2001-2010年9种不同土地利用类型产生的降雨径流及总氮和总磷的污染负荷,模拟结果为:流域内多年平均径流量为6 150万m3/a,总氮输出负荷为270 t/a,总磷负荷为22 t/a。模拟结果表明:水产养殖塘和高地茶果树是东山地区主要的营养物来源。通过三个情景方案的模拟,说明当地环境管理方案可以有效地削减入湖的营养负荷,其中第二个情景方案的削减量最大,总氮和总磷负荷分别削减了18%和25%。  相似文献   
4.
全自动阵列离子迁移谱仪连续监测挥发性有机化合物   总被引:1,自引:0,他引:1  
为了拓宽离子迁移谱仪的检测范围、提高化合物的识别准确度,研制了一套阵列离子迁移谱仪,该仪器基于63 Ni源正离子模式、63Ni源负离子模式和真空紫外灯光电离模式的组合电离源,可以连续监测空气中挥发性有机化合物.仪器采用全自动的采样进样系统,同时检测了二甲基亚砜的正离子和二氯甲烷的负离子,实现了正负离子的同时检测.通过对阵列离子迁移谱图的综合解析,识别了63Ni源正离子模式下难以鉴别的丙烯腈、间二甲苯和丙酮.连续4 d定量测定丙酮样品,结果表明仪器对丙酮的线性检测范围为2个数量级,线性相关系数R优于0.995,相对标准偏差控制在4.0%~18.3%.采用动态跟踪法,连续24 h在线监测了模拟泄漏的丙烯酸甲酯,监测结果直接反映了其泄漏的时间和浓度.  相似文献   
5.
新疆塔里木河流域的胡杨资源在维持荒漠生态系统的协调稳定和保障绿洲生态安全等方面意义重大。运用GIS技术、多媒体技术、数据库管理技术的理论与方法,综合胡杨资源及其环境的遥感信息、文字信息及地图信息,建立集图象、图形、声音、文字为一体的计算机信息管理系统,为胡杨资源信息的存贮、查询、检索、更新、分析服务,同时,为胡杨资源合理利用及保护提供科学依据及决策支持。  相似文献   
6.
Cryptosporidium and Giardia are two typical species of pathogenic protozoans that seriously endanger water quality. Previous works indicated that detection of Cryptosporidium and Giardia with modified United States Environmental Protection Agency (USEPA) method-1623 using a membrane filtration-elution for sample concentration attained better recovery and lower cost compared to the USEPA method-1623. Several improvements of membrane filtration-elution step as well as immunomagnetic separation (IMS) steps were investigated and an optimized method for detection of Cryptosporidium and Giardia in wastewater reclamation system was recommended in this paper. The experimental results show that an overnight soak of the membrane after scraping and vortex agitation before elution could enhance and stabilize the recovery. Increasing turbidity to 4 NTU by adding kaolin clay before filtration could effectively improve the recovery of low-turbidity water. Washing the concentrate after centrifugation and twice acid dissociation both reduced the impact of water quality to protozoan recovery. Protozoans in different water samples were determined by this optimized method, and the recovery of Cryptosporidium and Giardia were above 70% and 80% respectively, much higher than the acceptance of method-1623. __________ Translated from Environmental Science, 2006, 27(12): 2547–2552 [译自: 环境科学]  相似文献   
7.
油田采出水膜法处理技术应用研究进展   总被引:2,自引:0,他引:2  
油田采出水的处理回用是一种必然的趋势.膜法处理技术可以解决传统水处理中存在的问题,有效地去除采出水中的油类、细菌、硬度,防止油层堵塞、结垢和外排水的环境污染,对于促进油气田可持续发展、水资源再利用和环境保护具有非常重要的意义.微滤、超滤和纳滤、反渗透膜技术在油田采出水处理中已有较多的应用研究.超滤在除油和除浊方面表现出...  相似文献   
8.
N. Hamidi  M. Meftahi 《Safety Science》2012,50(5):1180-1189
Nowadays, with implementation of management systems and environment management and due to the influence of safety and health issues on working processes, organizations have also sought to acquire health and safety management systems. This study which aims to examine the influence of integrated management system on safety and productivity indices has retrospective experimental nature. It was conducted in Cement Factories in which three systems are used: quality, environment and safety systems. The collected data were: accident reports and the investigation of events in 6 years during 2005-2010 (3 years before and 3 years after the implementation of the system). The safety indices that used in this study are: IFR, ISR, FSI, and Safe T. Score. Then, the data analyzed with the T-test, mean and standard deviation. The results showed a significant difference between various safety indices before and after the implementation. The examination of production indices such as increasing rate of productivity and production indicates the influence of these systems on production and productivity indices. The results have showed that the safety system existence cannot ensure productivity increase. Indices definition can be helpful for the safety system effectiveness and system continual improvement. It is important to say that there might be various indices definition in different industries.  相似文献   
9.
A worldwide radionuclide network of 80 stations, part of the International Monitoring System, is being setup to monitor compliance with the Comprehensive Nuclear-Test-Ban Treaty (CTBT). The radioactivity sampled at these stations is primarily 220Rn progenies affecting the detection capability. A model linking the 220Rn emanation with the sampled 212Pb activity was developed and is presented here. The model and the performed measurements show that the variation of the sampled 212Pb activity can be fully explained by the variation of the local 220Rn activity concentration.  相似文献   
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