Factors driving the seasonal dynamics of Pseudo-nitzschia species and domoic acid at mussel farming in the SW Mediterranean Sea

ABSTRACT

The seasonal variations in Pseudo-nitzschia species and domoic acid (DA) concentration were investigated, at three shellfish farms in SW coastal Mediterranean. In parallel, the toxicity of mussels was tested. Two distinct groups of species were enumerated according to morphology and size (Pseudo-nizschia delicatissima and P. seriata groups). DA was detected over a nine-week period from July to October 2012 in the Lagoon, with a maximum concentration recorded in July (12.71?ng?DA?L^?1). DA was positively correlated with the presence of P. seriata-group and P. delicatissima-group and was mostly occurred during P limitation period in seawater. No DA was found in mussels that were collected during the period of DA absence in seawater. Our results suggest that temperature, salinity, inorganic and organic nutrients were significant for the seasonal dynamics of P. seriata and P. delicatissima groups, but that the P limitation was the most driving factor for DA production in these areas. The relative influence of environmental factors should be further studied to better understand the recent surfacing of massive blooms of toxigenic Pseudo-nitzschia in SW Mediterranean coast.     

氮磷等营养盐对尖刺拟菱形藻生长的影响

尖刺拟菱形藻Pseudo-nitzschiapungensHalse是我国沿海常见的赤潮生物。通过对采集和分离于珠江口大亚湾海域的尖刺拟菱形藻藻种,在实验室条件下,研究不同浓度氮、磷等营养盐限制下该藻生长增殖的关系和特征,以及氮磷比(c(N)/c(P))变化等对该藻生长的影响。实验结果表明:尖刺拟菱形藻对氮、磷的依赖性较强,随浓度的变化而变化,属于营养依赖型藻类。实验进一步发现,尖刺拟菱形藻的生长对氮、磷的需求并不是越多越好,而要求有一定的比例范围,得出c(N)/c(P)在10～32范围内有相对较大的生长,而当c(N)/c(P)<5或c(N)/c(P)>100时,尖刺拟菱形藻的生长明显受到抑制。     

温度和光照对两株赤潮硅藻生长的影响

利用多因子实验设计,研究了主要环境因子温度、光照对尖刺拟菱形藻(Pseudo-nitzschia pungens)和中肋骨条藻(Skeletonema costatuma)生长的影响。经ANOVA统计分析(Tukey test),结果表明,在实验范围(温度15,20,25,30℃和光照强度1500,4000,7000lx)内,温度对尖刺拟菱形藻的生长有显著影响(0.01相似文献