The effect of the introduction of prenatal diagnosis on the reproductive history of women at increased risk from neural tube defects

The reproductive history of 45 couples at increased risk for neural tube defect (NTD) who came for genetic counselling in 1970 and 1971 were compared with a similar number counselled in 1975 and 1976, when prenatal diagnostic tests were freely offered. They were subsequently interviewed in their homes and had their reproductive history recorded to the end of 1973 and 1978 respectively. Nearly all had a previous child with an NTD and none of the women were pregnant at the time of counselling. The effect of prenatal diagnosis was to speed somewhat the decision about further pregnancies, but the number of couples deciding on no further children and on having further pregnancies were almost identical in the two groups. The average number of pregnancies was 2·8 per family, with only 1·2 surviving children. The pregnancy outcomes are discussed as are the reasons for not attempting further pregnancies in both groups, which included very high risk of recurrence, a surviving spina bifida child, inability to accept the tests or its implications. Ninety per cent of the second group had tests. Their reactions to the tests were favourable but all complained of the waiting time between amniocentesis and obtaining the results. They all would have tests again in any future pregnancy. The reason for women not having prenatal diagnostic tests included inability to accept termination. It is concluded that couples in South Wales decide either to have no more children or to have further pregnancies regardless of tests. but tests speed a decision and enable the women to enjoy the pregnancy after obtaining the results, and that an NTD greatly reduces the number of children per family. A termination for an NTD is much more acceptable to most than an NTD at term. The reasons for this are discussed.     

环境化学污染对人类的生殖危害

目前认为，环境化学物污染已对人类生殖造成一定的有害影响，综述近年来国内外有关环境中金属元素、有机化学品、气体及农药等化学物质对人类生殖影响的研究报道，为进一步开展环境生殖研究提供参考。     

The adaptive significance of male polymorphism in the acarid mite Caloglyphus berlesei

Summary Two forms of males occur in the acarid mite, Caloglyphus berlesei. One of them uses its thickened third pair of legs to kill other males; the other, without modified legs, does not attack other males. Previous studies have shown that the form of the male is environmentally determined: fighter males develop only at low population densities and their development can be suppressed by substances emanating from dense colonies. In this study, the duration of development, longevity and virility of the two male morphs were measured. The only significant difference was that fighters had a shorter development time between the two last moults. In another experiment, two groups of colonies were maintained under the same conditions but had different numbers of individuals: small colonies contained 2 fighters and 2 non-fighters, whereas large colonies contained 30 males of each type. The relative reproductive success of both morphs was estimated from numbers of matings. In small colonies, the estimated reproductive success (ERS) was significantly higher for fighters than for non-fighters, mainly because in over 50% of these colonies a single fighter male managed to kill all the rival males and monopolize the females. The opposite was true in large colonies, in which non-fighters achieved significantly higher ERS, mainly because the fighters were killed in fights more often than the non-fighters. This implies that the ratio of costs to benefits obtained by adopting the fighter-male strategy increases with the number of rival males, which may explain conditional male development in C. berlesei.     

Reproductive success increases with degree of kinship in cooperative coalitions of female red howler monkeys (Alouatta seniculus)

Evaluation of evolutionary mechanisms proposed to promote cooperative behavior depends on the relative influence of the behavior on the reproductive success of individuals, the reproductive success of the group in which they interact behaviorally, and the degree of gene correlation among cooperators. The genetic relationship within cooperative coalitions of female red howler monkeys was examined for three populations with different densities and growth rates. Patterns of gene correlation change within coalitions is documented using data from the mitochondrial and nuclear genomes, and long-term census monitoring. Differences in fecundity and infant survivorship within and between groups of unrelated (rˉ=0) and related (rˉ≥ 0.25) females are compared. Females that emigrate from their natal groups form coalitions with other migrant females. These coalitions attempt to establish a territory and, once successful in producing offspring, exclude other females from feeding resources. Females in these coalitions had different mtDNA haplotypes and a genetically estimated mean r of 0, supporting demographic data on emigration patterns indicating that these females rarely have the opportunity to form coalitions with kin. Patterns of recruitment and rate of matriline development within social groups supported behavioral data indicating that females actively attempt to promote their own matriline as breeders over that of other females, and that some matrilines are more successful at this than others. Mean r among females was significantly higher in coalitions established as social groups for several generations (rˉ=0.44). In these groups, females all shared the same mtDNA haplotype, and mtDNA haplotype divergence was significantly higher between than within groups. Females in coalitions with kin had significantly higher reproductive success than females in unrelated coalitions in all populations. This difference was not a function of coalition size, number of males, socionomic sex ratio, or primiparity, although anecdotal evidence suggests that allomothering may compensate for inept new mothers in related coalitions more often than in unrelated ones. Differences in territory quality could not be ruled out as a potential causal factor in the saturated populations, but were unlikely in the low-density, growing population. There were substantial differences among long-established coalitions in overall reproductive output in all three populations, and this was significantly correlated with the number of breeding females. Increase in coalition size was a function of both group age and the behavioral tolerance among females. Regardless of the underlying reasons for the patterns observed, reproductive success clearly increases with degree of gene correlation among females within cooperative coalitions, and coalitions that recruit more daughters produce more offspring. The nature of the cooperative relationship among group females directly influences both of these outcomes. This is associated with substantial genetic differentiation among social groups within populations, creating conditions in which genetic tendencies towards cooperative behavior can become tightly associated with group reproductive success. Received: 15 September 1999 / Revised: 27 April 2000 / Accepted: 27 May 2000     

Multiple song repertoire characteristics in the willow warbler (Phylloscopus trochilus): correlations with female choice and offspring viability

Male willow warblers have song repertoires which vary in complexity along several dimensions. We examined whether female choice, as measured by date of pairing, was based on these song characteristics in 4 different years. Pairing date was negatively correlated with song repertoire size in 1 year, and with song versatility in another year, but there was no consistent effect of any song characteristic on pairing throughout the years or in the pooled sample. The variable that best explained how soon a male pairs is male arrival date (only males that had settled territories before the first female arrived were considered in the analysis). This correlation is consistently significant in all years. This is most parsimoniously interpreted as females choosing some habitat characteristic in the same way that males do. A small percentage of males (8.3%) attracted and paired with a second female. The likelihood of becoming polygynous was not explained by any measured song characteristic, but it was related to arrival date: early males were more likely to pair with two females. Males with large repertoires fledged more young in their primary nests, and there was a trend for the offspring of these males to have a greater probability of being recruited into the population. In conclusion, the results show that in most years there is no sexual selection by female preference on song characteristics, although the data on reproductive success is consistent with the idea of repertoire size being an indicator of male quality. Received: 4 June 1999 / Received in revised form: 1 December 1999 / Accepted: 31 December 1999     

Social and mating system of cooperatively breeding laughing kookaburras (Dacelo novaeguineae)

DNA fingerprinting was combined with field observations over four breeding seasons to investigate the social structure and mating system of the laughing kookaburra (Dacelo novaeguineae). Groups comprised a socially dominant pair and up to six helpers of either sex. Helpers were always recruited from young hatched in the group. Territorial inheritance, which is a feature of other cooperative breeders and an oft-cited benefit of philopatry, did not occur. Helpers only attained dominant status in an established group by dispersing into a vacant dominant position in that group. However, helpers could also form new groups by excising a new territory, often through a ”budding” process. The mating system was overwhelmingly monogamous. There were no cases of extra-group parentage in a sample of 140 nestlings; within groups of three or more birds, dominance predicted parentage almost perfectly (99.2% of 129 nestlings), irrespective of whether helpers in the group were related to one or both dominant birds. This is contrary to predictions from models of reproductive skew, possibly because they currently fail to incorporate the willingness of females to share reproduction among males. Received: 15 May 1999 / Received in revised form: 2 November 1999 / Accepted: 6 November 1999     

Aggression and resource sharing among foundresses in the social wasp Polistes dominulus: testing transactional theories of conflict

Tug-of-war models of within-group conflict predict that the frequency of conflict will be positively related to the degree of reproductive sharing within the group; in contrast, a negative relationship supports transactional models, in which reproductive payments among group members limit the degree of within-group selfishness. We tested predictions of the tug-of-war and transactional models by examining cofoundress interactions during the founding (preworker) phase of colony development in 30 naturally nesting colonies of the paper wasp Polistes dominulus. We found that the mean rate of foundress aggression and the mean probability of food sharing were significantly negatively associated, which supports the prediction of the transactional, not the tug-of-war model. Further, cofoundress aggression significantly increased over the founding phase (independently of temperature), while the fraction of aggression initiated by the dominant (alpha) foundress significantly decreased over this period. We show that both of these results are predicted by the transactional model of within-group conflict. Interestingly, the alpha’s rate of aggression was significantly positively temperature dependent, while the beta’s was not. This indicates that the alpha’s aggression level may often be near her physiological maximum, while the beta’s aggression is limi- ted by other factors, contradicting the prediction of the tug-of-war model. Moreover, the alpha’s aggression was significantly positively temperature dependent only in the second half of the founding period, as predicted by the transactional model since this is when there is least reproductive sharing. Finally, our results indicate that the alpha’s level of aggression depends on the resources controlled by the beta. Received: 18 January 2000 / Revised: 19 June 2000 / Accepted: 24 June 2000     

Reproductive success of monogamous and polygynous pied flycatchers (Ficedula hypoleuca)

One of the most interesting aspects of the mating system of pied flycatchers is the regular occurrence of polygyny. Here we present data on the reproductive success of polygynously paired pied flycatcher males compared to monogamous males based on paternity analyses through DNA fingerprinting. Males paired with two females suffered a higher loss in reproductive output per female compared to monogamous males due to (1) a greater proportion of unhatched eggs in their broods, (2) greater nestling mortality and (3) a greater probability of being cuckolded. Nevertheless, the number of fledglings was significantly greater for polygynous males. Based on the number of nestlings that returned for subsequent breeding seasons, however, the reproductive success of monogamous and polygynous males did not differ significantly. These data raise the question as to why males attempt polygyny. Received: 16 August 1999 / Received in revised form: 15 March 2000 / Accepted: 18 March 2000     

Diversity, Seasonality, and Context of Mammalian Roadkills in the Southern Great Plains

Thousands of mammals are killed annually from vehicle collisions, making the issue an important one for conservation biologists and environmental managers. We recorded all readily identifiable kills on or immediately adjacent to roads in the southern Great Plains from March 2004–March 2007. We also recorded distance traveled, whether a road was paved or divided, the number of lanes, and prevailing habitat. Surveys were opportunistic and were conducted by car during conditions of good visibility. Over our 239 surveys and >16,500 km traveled, we recorded 1412 roadkills from 18 different mammal species (size ranged from Sciurus squirrels to the white-tailed deer, Odocolieus virginianus). The overall kill rate was 8.50 / 100 km. Four species were prone to collisions: the Virginia opossum (Didelphis virginiana), nine-banded armadillo (Dasypus novemcinctus), striped skunk (Mephitis mephitis), and northern raccoon (Procyon lotor). Together they accounted for approximately 85% (1198) of all roadkills. Mortality rate differed significantly between 2- and 4-lane roads (8.39 versus 7.79 / 100 km). Kill rates were significantly higher on paved versus unpaved roads (8.60 versus 3.65 / 100 km), but did not depend on whether a road was divided. Roadkills were higher in spring than in fall (1.5×), winter (1.4×), or summer (1.3×). The spring peak (in kills / 100 km) was driven chiefly by the armadillo (2.76 in spring/summer versus 0.73 in autumn/winter) and opossum (2.65 versus 1.47). By contrast, seasonality was dampened by a late winter/early spring peak in skunk mortalities, for which 41% occurred in the 6-week period of mid-February through March. The raccoon did not exhibit a strong seasonal pattern. Our data are consistent with dispersal patterns of these species. Our results underscore the high rate of highway mortality in the southern plains, as well as differences in seasonality and road type that contribute to mortality. Conservation and management efforts should focus on creating underpasses or using other means to reduce roadkill rates.     

Mating patterns and reproductive success in the bushy-tailed woodrat (Neotoma cinerea), as revealed by DNA fingerprinting

The mating patterns and reproductive success of the bushy-tailed woodrat (Neotoma cinerea) were investigated over a 3-year period (1992–1994) using DNA fingerprinting. Paternity was determined by genetic analysis of 58 juveniles of known maternity from 35 litters. Analysis of DNA fingerprints revealed that all offspring within a litter were fathered by a single male; the statistical probability of detecting multiple males mating with a female was high, indicating that multiple paternity would have been detected had it occurred. However, individual males did not father more than one litter from a given female either within or between years. At least 75% of females and 57% of males successfully produced offspring each year. The finding that all littermates are first-order relatives may contribute to the high level of female cooperation in this species. Received: 28 May 1997 / Accepted after revision: 22 March 1998