鳗鲡仔鱼(Anguilla japonica Temn.et Schl.)对铜离子的回避反应

本文力图用回避实验的方法分析铜离子对闽江鳗鲡及其他水产资源的影响。实验在流水中进行,温度为16±0.5℃,pH为7.43±0.2,溶解氧为62—68.9％;鱼体长为46—69.5mm,重量为174.2—226.6mg;每一浓度测试20次。当浓度为0.0001ppm时,回避度为12.81％;浓度为0.016ppm时,有明显的回避反应,回避度为58.28％;浓度升至0.064ppm时,回避度84.74％,为完全回避。     

室内培养底栖端足类日本大螯蜚饵料研究

本文在实验条件下探讨了５种饵料对海洋底栖端足类日本大螯蜚的培养效果。实验指标为存活率，生长速度，日增长率和发育状况。结果表明，青岛大扁藻与新月菱形藻１：１混合液的培养效果最佳，合成饲料是较好的补充饵料，螺旋藻粉不宜作为日本大螯蜚的培养饵料。动物性饵料培养效果不如植物性饵料，但对日本大螯蜚的早期快速成长具有一定的促进作用。     

酸碱度变化对日本三角涡虫生存及再生的影响

用自来水和去离子水配制不同的pH溶液，测定涡虫生存及再生的情况，以及涡虫可承受的pH变化幅度，确定日本三角涡虫对不同酸碱环境的耐受性。结果表明涡虫对酸碱环境的耐受力较强。     

Production and characterization of biodiesel from Eriobotrya Japonica seed oil: an optimization study

Biodiesel is now-a-days recognized as a real potential alternative to petroleum-derived diesel fuel due to its number of desirable characteristics. However, its higher production cost resulting mainly due to use of costly food-grade vegetable oils as raw materials is the major barrier to its economic viability. Present work is an attempt to explore the potential of Eriobotrya japonica seed oil for the synthesis of biodiesel using alkali-catalyzed transesterification. Optimization of production parameters, namely molar ratio of alcohol to oil, amount of catalyst, reaction time and temperature, was carried out using Taguchi method. Fatty acid composition of both oil and biodiesel was determined using GC and H¹ NMR. Alcohol to oil molar ratio of 6:1, catalyst amount of 1% wt/wt, 2 h reaction time and 50 ^°C reaction temperature were found to be the optimum conditions for obtaining 94.52% biodiesel. Highest % contribution was shown by the ‘amount of catalyst’ (67.32%) followed by molar ratio of alcohol to oil (25.51%). Major fuel properties of E. japonica methyl esters produced under optimum conditions were found within the specified limits of ASTM D6751 for biodiesel, hence it may be considered a prospective substitute of petro-diesel.     

Low uptakes of Cd,Cu, and Zn in Dugesia japonica,a freshwater planarian with higher tolerance to metals

Although a freshwater planarian is proposed as a potential model for studying toxicities of environmental pollutants, they are less sensitive to metal exposures, compared to other freshwater invertebrates. We hypothesised that the metal uptake is low in treated planarians, which may lead to their higher tolerance to metals. The acute toxicities of Cu and Zn to Dugesia japonica were determined. The 24- and 48-h median lethal concentrations were 4.50 and 3.46?mg?L^?1, respectively, for Cu, and 41.97 and 37.79?mg?L^?1, respectively, for Zn. Accordingly, combining with the data on Cd in our previous study, higher tolerance of planarians to metals was revealed. Also, tissue levels of Cd, Cu, and Zn in treated D. japonica were determined, and their dissolved uptake rate constants (k₁) were calculated. Results showed that bioaccumulations of these metals in D. japonica were, indeed, lower than that in other freshwater invertebrates having higher susceptibilities to these metals.     

Matrine and oxymatrine in corroborant plant extracts and fertilizers: HPLC/MS-MS method development and single-laboratory validation

A reversed phase high-performance liquid chromatographic method (HPLC/MS-MS) has been developed and validated for detection of alkaloids matrine and oxymatrine in fertilizer with labeled enhancer plant defense activities. The analytical method was validated statistically. The results show a strong matrix effect, requiring quantification by standard addition method. The regression lines showed r² > 0.994. Recoveries ranging from 97 to 104% were obtained for the fortification level of 0.01% wt wt^?1 and the relative standard deviations ranged from 3 to 4% (n = 10). The limits of detection were below 0.0001% wt wt^?1, while the limits of quantification did not exceed 0.0004% wt wt^?1. The method is currently applied in ICQRF Laboratory of Catania on fertilized and corroborant plant extract collected in the Italian market in the frame of MIPAAF institutional quality control activity, with the aim to dectect these unpermitted active substances.     

农业技术进步、规模效率与粮食安全——以东北三省粳稻、玉米为例

以东北三省粳稻、玉米为例,使用DEA-malmquist指数法实证检验农业技术进步、规模效率与粮食生产效率增长之间的关系。结果发现:①2000—2019年,吉林和黑龙江均保持了良好的粮食生产效率。②黑龙江属于"农业技术进步单一驱动型"的生产模式,而辽宁、吉林属于"农业技术进步和技术效率双驱动型"的生产模式。③吉林玉米生产的规模效率非有效,辽宁农业技术进步的衰退分别阻碍了各自粮食生产效率的提高。基于以上研究,提出建议:黑龙江、吉林、辽宁3省应因地制宜,分别通过增加农业财政投入、加强农业科技研发力度、鼓励农地流转等措施为粮食安全保驾护航。     

亚硝态氮胁迫对日本蟳消化酶活力及同工酶表达的影响

亚硝态氮是养殖水体中常见的胁迫因子.为探讨亚硝态氮对养殖蟹类的毒性作用,采用生物酶测定及聚丙烯酰胺凝胶电泳的方法,研究了水体中不同浓度(0.15,0.3,2,5,10,20mg/L)亚硝态氮胁迫下日本蟳肝胰腺消化酶活力的变化,以及高浓度(20mg/L)亚硝态氮胁迫对日本蟳同工酶表达的影响.结果表明:低浓度(0.15,0.3mg/L)的亚硝态氮胁迫对碱性蛋白酶,酸性蛋白酶,脂肪酶和淀粉酶活力均产生一定的诱导效应,且在处理7d时,除淀粉酶活力略低于对照组外,其他3种消化酶活力仍保持在较高的水平.较高浓度(2,5,10mg/L)亚硝态氮的短期胁迫(0.5~1d)会诱导酸性蛋白酶,碱性蛋白酶和脂肪酶活力的迅速升高,但随即快速下降.高浓度(20mg/L)的亚硝态氮胁迫对蛋白酶和淀粉酶活力表现出明显的抑制效应.处理7d时,胁迫浓度2mg/L及以上的实验组各消化酶活力与剂量浓度间均呈显著负相关关系.20mg/L亚硝态氮胁迫下,除肌肉中新增两条酶带(MDH-2和MDH-4)外,日本蟳鳃,肝胰腺,胃,心脏,卵,精子等组织中的α-淀粉酶(α-AMY)同工酶,乳酸脱氢酶(LDH)同工酶,苹果酸脱氢酶(MDH)同工酶和过氧化物酶(POD)同工酶均出现活性减弱或酶带数量减少现象.结果显示高浓度亚硝态氮胁迫对日本蟳消化酶活力和同工酶表达均表现出明显的抑制作用.     

东亚三角涡虫监测水体重金属污染的可行性研究

随着现代工、农业的快速发展,水体重金属污染已成为全球性的环境问题。对水体重金属污染的现状、来源、危害以及监测方法进行了介绍,并探讨了生物监测技术的优越性。在前人的研究基础上,结合本实验室工作,提出利用东亚三角涡虫(Dugesiaojapoice)作为水体重金属污染监测指示生物的可行性,并对更为灵敏的生物标志物的策略和实验方案进行了展望。     

大亚湾日本星杆藻种群动态及其与环境因子的关系

研究了1997～1998年及1999和2000年春季大亚湾日本星杆藻(Asterionella japonica)种群动态及其与环境因子之间的关系.日本星杆藻是大亚湾浮游植物常见优势种类,可在全年温度范围(14～32.8℃)内形成数量高峰,并发生水华.日本星杆藻在1998年和2000年春季不常出现,但1999年春丰富的营养盐含量及它们之间合适的比值、多变的水温、稳定的盐度和晴朗的天气使日本星杆藻迅速生长,并发生了水华,最高细胞密度达5255cells/mL.水华消耗了大量的营养,水华期间DIN、DIP和Dsi分别下降了76.0%、69.7%和58.8%,而且营养元素的消耗特别是DIN的耗尽是水华消退的重要原因,而桡足类等浮游动物摄食也是水华消退的原因之一.