COMBUSTIVE APPROACH FOR MEASURING TOTAL VOLATILE PHOSPHORUS CONTENT IN LANDFILL GAS

A technique was developed to measure the total gaseous phosphorus content in biogas. The amount of air needed for a neutral to oxidising flame was mixed with the biogas. The gas mixture was burnt in a closed quartz burner and the combustion gasses were bubbled through a nitric acid solution. The phosphate content in the bubbling liquid was determined with sector field ICP-MS. The technique was validated in the lab with phosphine. Afterwards the set-up was installed on a landfill. The total gaseous phosphorus content in the landfill gas, measured with the combustive technique, ranged from 1.65 to 4.44 g P/m³. At the same time the phosphine concentration in the landfill gas was determined gas chromatographically (GC). The phosphine (PH₃) content measured with GC ranged from 7.6 to 16.7 g PH₃-P/m³. Since the phosphine-P content (GC) was consistently higher than the total gaseous phosphorus content (burner/ICP-MS), the hypothesised presence of highly toxic gaseous phosphorus compounds other than phosphine could not be demonstrated.     

Influence of glucose feeding on the ligninolytic enzyme production of the white-rot fungus Phanerochaete chrysosporium

The present work studied the influence of glucose feeding on the ligninolytic enzyme production of Phanerochaete chrysosporium in a nitrogen-limited (C/N ratio is 56/8.8 mmol/L) medium. Several sets of shaking flask experiments were conducted. The results showed that 2 g/L glucose feeding on the first day of the culture (24 h after the inoculation) simulated both fungal biomass growth and enzyme production. The manganese peroxidase (MnP) activity was 2.5 times greater than that produced in cultures without glucose feeding. Furthermore, the glucose feeding mode in fed-batch culture was also investigated. Compared to cultures with glucose feeding every 48 h, cultures with glucose feeding of 1.5 g/L (final concentration) every 24 h produced more enzymes. The peak and total yield of MnP activity were 2.7 and 3 times greater compared to the contrast culture, respectively, and the enzyme was kept stable for 4 days with an activity of over 200 U/L. Translated from Acta Scientise Circumstantiae, 2007, 27(3): 363–368 [译自: 环境科学学报]     

Connecting sources, fractions and algal availability of sediment phosphorus in shallow lakes: An approach to the criteria for sediment phosphorus concentrations

Although point and nonpoint sources contribute roughly equal nutrient loads to lakes, their relative role in supporting algae growth has not been clarified. In this research, we have established a quantitative relationship between algae-available phosphorus (P) and P chemical fractions in sediments; the latter indicates the relative contribution of point versus nonpoint sources. Surface sediments from three large shallow lakes in eastern China, namely, the Chaohu, Taihu and Hongzehu Lakes, were sampled to assess their algae-available P and chemically extracted P fractions. The algae-available P primarily comes from iron/aluminium (hydr)oxide-bound P (Fe/Al-bound P), 45% of which is algae-available P. The ratio of Fe/Al-bound P to calcium compound-bound P (Ca-bound P) indicated the relative contribution of point to nonpoint sources, with the point sources contributing the majority of increased Fe/Al-bound P in sediments. Therefore, the reduction of point sources from urbanized areas, rather than nonpoint sources from agricultural areas that primarily contribute to the Ca-bound P fraction, should be prioritized to alleviate cyanobacterial algal blooms (CyanoHABs) in shallow lakes with sediment P as a potential source to support algae growth. With these important results, we proposed a conceptual model for “P-pumping suction” from sediments to algae to aid in the development of the criteria for sediment P concentrations in shallow lakes.     

软式排球健身价值的研究

运用文献、调查访问、观察、实验、统计及分析等研究方法,对有、无排球技术基础学生的对比分析,探讨软式排球的健身价值。研究结果表明:软式排球较硬式排球技术更易学习掌握,更易在学生中组织比赛,有着很重要的健身价值。为使软式排球更具有健身和娱乐性,应在比赛中灵活制定比赛规则。     

Activity of biotin-dependent and gaba metabolizing enzymes in chorionic villus samples: Potential for 1st trimester prenatal diagnosis

We have documented the presence of five mitochondrial enzymes in samples of chorionic villus tissue and measured the levels of activity. Three of the enzymes catalyse biotindependent reactions. These are propionyl-CoA carboxylase, 3-methylcrotonyl-CoA carboxylase and pyruvate carboxylase. the other enzymes. 4-aminobutyric acid aminotransferase and succinic semialdehyde dehydrogenase, are involved inthe degradation of the central inhibitory neurotransmitter GABA. Distinct diseases in whichthere is deficiency of each of these enzymes have been documented in man. Significant levels of activity were observed for all five enzymes in chorionic villus tissue. This methodology should permit early prenatal diagnosis of deficiencies of these enzymes by chorionic villus biopsy in the first trimester.     

3种重金属对青萍毒害的研究

对水体中主要的3种重金属(铜、镉、铅)对青萍抗氧化酶系统的几种酶,包括过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性,丙二醛(MDA)的含量,可溶性蛋白含量及叶绿体色素含量等指标的影响进行了研究.研究表明青萍对镉污染反应最为敏感,对铜的反应相对缓和一些;青萍在铅的胁迫下表现出无序的状态.因此,青萍不适合应用于重金属镉污染的富营养化水体的治理和生态修复中.     

沉淀—树脂吸附法处理对氨基偶氮苯盐酸盐生产废水的研究

对氨基偶氮苯盐酸盐（ＰＡＢＳ）是生产染料的重要中间体。在ＰＡＢＳ生产过程中，排放出高浓度（ＣＯＤ４万～８万ｍｇ／Ｌ）、高色度（４万～８万）、高毒性（含苯胺和对氨基偶氮苯）和高氯（Ｃｌ－４万～１０万ｍｇ／Ｌ）的废水。利用中和沉淀—大孔吸附树脂吸附法进行了处理工艺的小试研究。结果表明，废水色度去除率达到９９９％，降到２０以下；出水中基本不含对氨基偶氮苯（ＰＡＢ）；出水中苯胺的浓度降到３ｍｇ／Ｌ。ＧＣ／ＭＳ分析的结果表明，乙醇脱附液中苯胺是最主要的有机物，所以利用该工艺能实现苯胺的回收。     

Influence of no-tillage on the distribution and lability of phosphorus in Finnish clay soils

No-tillage (NT) is a method adopted to reduce erosion and particulate phosphorus (P) load from arable land to watercourses. However, it has been found to increase the loss of dissolved P with surface runoff, but the reasons for that have rarely been examined in detail. The objective of the present study was to determine the chemical factors explaining this response by investigating the impact of NT on the type and distribution of P reserves as well as on organic carbon (C) in the 0–35 cm topsoil layer of clay soil profiles (Vertic Cambisols). Soil samples were taken from two experimental fields (Jokioinen and Aurajoki) at 0–5, 5–20 and 20–35 cm depths in conventionally tilled (CT) and non-tilled (for 4–5 years) plots. The plots had been cultivated and fertilized according to the common field practices in Finland (15–18 kg P and 100–128 kg N ha⁻¹ year⁻¹).Inorganic and organic P reserves characterized by a modified Chang and Jackson fractionation procedure were not significantly affected by the cultivation methods. However, in the uppermost soil layer (0–5 cm) in NT of the Jokioinen field, the labile P determined by water extraction (P_w) increased significantly, whereas the increase in P extracted with acid ammonium acetate (P_AAC) remained statistically insignificant. The increase in labile P coincided with a significant increase in organic carbon (C), which supports the theory that competition between organic anions and phosphate for the same sorption sites on oxide surfaces will enhance the lability of soil P. In the Aurajoki field with distinct soil cracking, P_w and P_AAC were not affected by NT in the uppermost soil layer, but they increased in the deepest soil layer (20–35 cm) concomitantly with an increase in Al-bound P and organic C. However, the increases were not statistically significant. In both fields, soil acidification due to the repeated application of N fertilizers at a shallow soil depth as well as the accumulation of organic C lowered pH of the uppermost soil layer in NT compared to the deeper soil layers. The results indicated that even short-term NT can increase the labile P in clay soil. However, further studies are needed to assess the long-term changes in lability of surface soil P and, consequently, the possible need for readjustment of the fertilization level in NT.     

半导体生产排放的废气和废水中砷、磷、硫的分析方法研究

研究了一种快速、灵敏、可靠的高温氢还原-气相色谱分析方法[1-2]，并自行设计了高温还原系统(还原炉、六通阀等)，测定半导体生产排放废气和废水中的砷、磷、硫及其化合物，样品不需预处理，使气相色谱仪能直接测定固、液、气相样品中砷、磷、硫及其化合物的含量。砷、磷、硫的检测限分别为0．01mg／L、0．003mg／L、0．02mg／L；相对标准偏差分别为6．2％、8．6％和0．3％，与经典方法进行对比，结果基本一致。从分析方法的误差统计角度，也说明该方法准确、可靠。     

青藏高原北缘P波波形线性度的动态跟踪及其变化的物理机制初探

利用数字化近震Ｐ波波形资料，追踪研究了青藏高原北缘１９９０年２月～１９９５年８月Ｐ波波形时间线性度的变化过程。发现该区较大地震发生前线性度有一定程度的下降现象。分析认为：大震前孕震区应力场的不均匀分布可使通过孕震区及其周缘的地震波产生频散，可能是较大地震前波形时间线性度下降的物理本质