Marsh Frog (Rana ridibunda Pall.) in Cooling Ponds in the Middle Urals

Environmental conditions in cooling ponds of thermal power plants are favorable for marsh frogs. Their populations inhabiting Verkhne-Tagil and Reftinskoe reservoirs differ in some traits, such as size and age composition, growth rate after metamorphosis, spawning type, fecundity, and characteristics of larval development. Differentiation in body size manifests itself at the early stages of terrestrial life and progresses at later stages. As a consequence, larger individuals may reach maturity and participate in reproduction at an earlier age.     

2,2’,4,4’-和3,3’,4,4’-四氯联苯(TCB)对小白鼠体外受精的影响(英文)

采用体外受精技术评价环境污染物 2 ,2’ ,4,4’ 和 3,3’ ,4,4’ 四氯联苯对小白鼠卵母细胞受精能力和 2 细胞胚胎发育的毒性作用 .实验结果表明 :ρ(PCB) =0 .1,1.0 ,10 .0mg/mL的 2 ,2’ ,4,4’ 和 3,3’ ,4,4’ 四氯联苯均显著降低小白鼠卵母细胞的受精能力 ;ρ(PCB) =1.0 ,10 .0mg/mL的 2 ,2’ ,4,4’ 和 3,3’ ,4,4’ 四氯联苯使卵母细胞的退化率和 2 细胞的畸形率显著增加 ;两种四氯联苯对小白鼠卵母细胞体外受精能力、退化率和 2 细胞胚胎的畸形率无统计差异 .表 2参 2 5     

Reference gene validation for quantification of gene expression during final oocyte maturation induced by diethylstilbestrol and di-(2-ethylhexyl)-phthalate in common carp

Final oocyte maturation is the key step to successful spawning and fertilization.Quantitative real-time PCR(q PCR) is the technique of election to quantify the abundance of functional genes in such study. Reference gene is essential for correct interpretation of q PCR data. However, an ideal universal reference gene that is stable under all experimental circumstances has not been described. Researchers should validate their reference genes while performing q PCR analysis. The expression of 6 candidate reference genes: 18 s r RNA,28 s r RNA, Cathepsin Z, Elongation factor 1-α, Glyceraldehyde-3-phosphate dehydrogenase andβ-actin were investigated during final oocyte maturation induced by different compounds(DES and DEHP) in common carp(Cyprinus carpio). Four softwares(Bestkeeper, ge Norm,Norm Finder and Ref Finder) were used to screen the most stable gene in order to evaluate their expression stability. The results revealed that EF1α was highly stable expressed when final oocyte maturation was induced by DES, while gapdh was the most stable gene when final oocyte maturation was induced by DEHP. Stable expressed reference gene selection is critical for all q PCR analysis to get accurate target gene m RNA expression information.     

香烟烟雾水溶物对小鼠卵母细胞体外减数分裂的影响

采用小鼠卵母细胞体外培养的方法研究了香烟烟雾水溶物对小鼠卵母细胞减数分裂的影响,以检查吸烟对生殖过程的影响。香烟烟雾水溶物可抑制卵母细胞排出第一极体,而不影响生发泡破裂。香烟烟雾水溶物抑制极体排出是由于破坏了细胞内的微丝或微管,使有丝分裂器不能向细胞边缘移动,并抑制了胞质分裂,形成两倍体的卵子。结果表明,吸烟对卵母细胞的减数分裂成熟有破坏作用。     

孕酮诱导非洲爪蟾卵母细胞生发泡破裂(GVBD)实验方法的优化及干扰物质筛查

非洲爪蟾卵母细胞生发泡破裂(GVBD)是生物学上研究卵细胞成熟过程及其机制的良好模型。由于GVBD受激素的调控,曾有研究将孕酮诱导的非洲爪蟾GVBD试验用于内分泌干扰物的筛查。通过预注射人体绒毛膜促性腺激素(HCG)提高非洲爪蟾卵母细胞对孕酮的敏感性、缩小个体差异,缩短试验时间等优化GVBD试验方法;并选用甲氧氯作为阳性参照物对优化的方法进行验证,最后用此方法研究了米非司酮、来曲唑、咪鲜胺3种化学物质对孕酮诱导GVBD的影响。结果发现,甲氧氯对GVBD的发生具有明显抑制作用,随着剂量的增加抑制作用增强,显示优化方法的可靠性。米非司酮作为孕酮受体拮抗剂,没有表现出抑制GVBD的效应,证明孕酮诱导爪蟾GVBD并非完全由孕酮受体(PR)介导。同时,来曲唑和咪鲜胺对孕酮诱导爪蟾GVBD没有影响,显示此两种物质在孕酮诱导GVBD过程中不具有内分泌干扰作用。     

铁钛共掺杂氧化铝诱发表面双反应中心催化臭氧化去除水中污染物

多相催化臭氧化技术因能有效去除水中有机污染物而受到广泛关注.然而,基于单一位点氧化还原的金属氧化物催化臭氧化过程存在速率限制步骤,使活性受到抑制,极大地限制了多相催化臭氧化技术的实际应用.为解决这一瓶颈,以过渡金属物种Fe和Ti对金属氧化物γ-Al₂O₃基底进行晶格掺杂制备出新型双反应中心催化剂FT-A-1 DRCs.通过XRD、TEM和XPS等技术对其形貌结构和化学组成进行了表征分析,证明Fe和Ti对于Al的晶格取代,形成表面贫富电子微区（富电子Fe微中心和缺电子Ti微中心）.FT-A-1 DRCs被用于催化臭氧化过程,对布洛芬等一系列难降解有机污染物的去除表现出优异的活性和稳定性.利用EPR和电化学技术揭示了界面反应机制.发现在催化臭氧化过程中,O₃/H₂O在富电子微中心被定向还原产生·OH,而污染物可在缺电子微中心作为电子供体而被氧化,为反应体系持续提供电子.这一反应过程利用污染物自身的能量实现了污染物的双途径降解（·OH攻击和直接电子供体）,突破了金属氧化物催化臭氧化过程存在速率限制步骤.     

Are motorcycles really “donorcycles”? Examining organ donation rates between unhelmeted and helmeted motorcyclists

Introduction: Motorcycles are colloquially referred to as “donorcycles” among medical staff. However, the actual impact of helmet laws and helmet use on organ donation is unknown. Michigan’s 35-year-old universal helmet law (UHL) was repealed in April 2012 and replaced by a partial-helmet law. We hypothesized that there would be an increase in organ donation rates from unhelmeted motorcyclist fatalities. Methods: Michigan's Gift of Life Michigan organ donation database was queried from April 2008 through May 2015 in conjunction with the Michigan Trauma Quality Improvement Program database from the same time period. All in-hospital motorcycle crash fatalities were examined. Results: A three-fold increase was found in the rate of organ donation for unhelmeted motorcyclists compared to helmeted motorcyclists (p = 0.006). Motorcycle crash fatalities tended to be younger in age after the UHL repeal with an average age of 32.8 years versus 40.8, however, this finding was not statistically significant (p = 0.071). Additionally, there was no significant difference in organ donation rates pre-UHL repeal (2008–2012) versus post-repeal (2012–2015). Conclusions: This is the first study to demonstrate an increased rate of organ donation among unhelmeted motorcyclist fatalities compared to helmeted rider fatalities. There was no significant increase in the rate of organ donation following the Michigan UHL repeal. However, we identified that some motorcycle crash fatalities were from illegally unhelmeted riders in the past, prior to the repeal. Practical Application: Unhelmeted motorcyclists are three times more likely than helmeted riders to become organ donors, possibly due to the well documented increase in severe traumatic brain injuries in this population. From a public health perspective, helmets should be required for all motorcyclists and efforts to advocate in favor of helmet legislation should be supported by trauma systems and health professionals.     

镉对小鼠卵母细胞成熟和体外受精的影响

采用小鼠卵母细胞体外培养、体外受精的方法研究了CdCl2对小鼠卵母细胞的生发泡破裂（GVBD）、第一极体释放及体外受精（IVF）的影响．结果表明,CdCl₂对GVBD没有影响,但可以抑制卵母细胞第一极体的释放,影响卵母细胞的存活率并可降低IVF频率CdCl₂影响极体释放和IVF率可能是其对纺锤体的破坏,使得减数分裂不能正常进行,同时还可能影响微丝等细胞骨架成分,阻止正常胞质分裂实验结果还显示,CdCl₂这种抑制作用可能是可逆的,或者仅仅是对减数分裂的延迟作用．     

Nature Documentaries,Connectedness to Nature,and Pro-environmental Behavior

We investigate the effects of nature documentaries on pro-environmental cognition and behavior. Of central interest is the concept of connectedness to nature, which describes an individual's sense of being connected to nature. Based on previous research showing that a direct nature experience in zoos can increase connectedness to nature, we questioned whether watching a nature documentary could increase connectedness to nature and pro-environmental behavior. An experiment using one control group (watching a documentary about Einstein's theory of relativity) and one treatment group (watching a nature documentary) revealed that a mediated nature experience is not sufficient to elicit an increase in connectedness to nature. However, we found that exposure increased actual donation behavior for animal and environmental protection organizations. It is important to note that nature documentary exposure increased pro-environmental donation behavior only in those already having a strong sense of connectedness. We discuss real-world implications for donation-accepting, non-profit organizations.     

Economic benefits from food recovery at the retail stage: An application to Italian food chains

The food supply chain is affected by losses of products near to their expiry date or damaged by improper transportation or production defects. Such products are usually poorly attractive for the consumer in the target market even if they maintain their nutritional properties. On the other hand undernourished people face every day the problem of fulfilling their nutritional needs usually relying on non-profit organizations. In this field the food recovery enabling economic benefits for donors is nowadays seen as a coherent way to manage food products unsalable in the target market for various causes and thus destined to be discarded and disposed to landfill thus representing only a cost. Despite its obvious affordability the food recovery is today not always practiced because the economic benefits that could be achieved are barely known. The paper aims at presenting a deterministic mathematical model for the optimization of the supply chain composed by retailers and potential recipients that practice the food recovery, taking into account the benefits recognized to donors and the management costs of the food recovery. The model determines the optimal time to withdraw the products from the shelves as well as the quantities to be donated to the non-profit organizations and those to be sent to the livestock market maximizing the retailer profit. The results show that the optimal conditions ensuring the affordability of the food recovery strategy including the tax reliefs and cost saving for the retailers outperforms the profit achievable in absence of such a system.