Biological Activity in a Heavily Organohalogen-Contaminated River Sediment (8 pp)

Background, Aims and Scope Sediments of the Spittelwasser creek are highly polluted with organic compounds and heavy metals due to the discharge of untreated waste waters from the industrial region of Bitterfeld-Wolfen, Germany over the course of more than one century. However, relatively few data have been published about the chloroorganic contamination of the sediment. This paper reports on the content of different (chloro)organic compounds with special emphasis on polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F), and chlorobenzenes. Existing concepts for the remediation of Spittelwasser sediment include the investigation of natural attenuation processes, which largely depend on the presence of an intact microbial food web. In order to gain more insight in terms of biological activity, we analyzed the capacity of sediment microflora to degrade organic matter by measuring the activities of extracellular hydrolytic enzymes involved in the biogeochemical cycling of carbon, nitrogen, phosphorus and sulfur. Furthermore, the detection of physiologically active bacteria in the sediment, particularly of those known for their capability to reductively dehalogenate organochlorine compounds, illustrates the potential for intrinsic bioremediation processes. Methods PCDD/F and chlorobenzenes were analyzed by gas chromatography(GC)/mass spectrometry and GC/flame ionization detection, respectively. The activities of hydrolytic enzymes were determined from freshly sampled sediment layers using 4-methylumbelliferyl (MUF) or 7-amino-4-methylcoumarin-conjugated model compounds and kinetic fluorescence measurements. Physiologically active bacteria from different sediment layers were microscopically visualized by fluorescence in situ hybridization (FISH). Specific bacteria were identified by 16S rRNA gene amplification and sequencing. Results and Discussion The PCDD/F congener profile was dominated by dibenzofurans. In addition, the presence of specific tetra and pentachlorinated dibenzofurans supported the assumption that extensive magnesium production was one possible source for the high contamination. A range of other chloroorganic compounds, including several isomers of chlorobenzenes, hexachlorocyclohexane and 1,1,1-trichloro-2,2-bis (p-chloro-phenyl)ethane (DDT), was present in the sediment. Activities of extracellular hydrolytic enzymes showed a strong decrease in those sediment layers that were characterized by high contents of absorbable organic halogen (AOX), indicating disturbed organic matter decay. Interestingly, an abnormal increase of cellulolytic enzyme activities below the organochlorine-rich layers was observed, possibly caused by residual cellulose from discharges of sulfite pulping wastes. FISH revealed physiologically active bacteria in most sediment layers from the surface down to the depth of about 60 cm, including members of Desulfitobacterium (D.) and Sulfurospirillum. The presence of D. dehalogenans was confirmed by its partial 16S rRNA gene sequence. Conclusions Results of chemical sediment analyses demonstrated high loads of organochlorine compounds, particularly of PCDD/F. Several years after stopping the waste water discharge to Spittelwasser creek, this sediment remains a main source for pollution of the downstream river system by way of the ongoing mobilization of sediment during high floods. As indicated by our enzyme activity measurements, the decomposition potential for organic matter is low in organochlorine-rich sediment layers. In contrast, the comparably higher enzyme activities in less organochlorine-polluted sediment layers as well as the presence of physiologically active bacteria suggest a considerable potential for natural attenuation. Recommendations and Perspectives From our data we strongly recommend to explore the degradative capacity of sediment microorganisms and the limits for in situ activity towards specific sediment pollutants in more detail. This will give a sound basis for the integration of bioremediation approaches into general concepts to reduce the risk that permanently radiates from this highly contaminated sediment. Submission Editor: Dr. Henner Hollert (Henner.Hollert@urz.uniheidelberg.de)     

平顶山市湛河水污染现状及防治对策

以“九五”期间平顶山市湛河水质例行监测结果为依据，分析了湛河水污染现状、特征及原因，并提出综合性湛河污染防治对策。     

低负荷间歇运行燃煤锅炉烟尘排放规律的探讨

通过对不同负荷情况下运行的燃煤锅炉烟尘排放浓度测试,探讨了低负荷间歇运行燃煤锅炉的烟尘排放规律.随着锅炉负荷的降低,过量空气系数变大,烟尘排放浓度和排放量也变大.在相近低负荷条件下运行的不同出力的锅炉,额定出力大的锅炉烟尘排放量大.在燃煤量相近而在不同负荷状态下运行的两台锅炉,额定出力大的锅炉烟尘排放量大.对于常年低负荷运行时的锅炉,环境管理部门在对其烟尘排放进行监督管理时宜以低负荷运行时的烟尘测试教据为依据.     

A routine method for storing lymphocytes for repeated isolation of DNA

A protocol for easy storage and later expansion of lymphocyte populations is given. Compared with methods using transformed cell lines, the method has a number of advantages for repeated production of cells for the isolation of DNA in amounts sufficient for use in diagnostic DNA technology.     

调引太湖水改善苏州市水动力条件研究

基于苏州地区独特的地理位置,简述引水改善该地区水环境的必要性,拟定了3种调水方案,利用感湖河网数学模型,对3种方案调水后主要河道的水动力条件进行预测,分析了各河道调水后的水动力变化趋势,提出了利用太湖水自流引水改善苏州市水环境的最佳方案.     

浅谈荆马河污水资源化生态工程

遵循可持续发展的原则，针对经济欠发达地区的水环境污染现状，提出了兴建产业化的污水处理生态工程，科学地发展污灌，既有效地改善了京杭运河（徐州段）的水环境质量，又使徐州市城市污水得以资源化利用，对促进环境与社会经济协调发展具有积极意义。     

昆明城市生态河道建设探讨

在城市河道治理过程中，由于只片面强调防洪、排水，忽略了河道的其他功能，致使河道越挖越宽、越挖越深、越修越直，而河水越来越臭、越来越脏。建设生态河道是解决这些问题的唯一出路，既要顾及河道排洪、防洪等基本功能，又要保持河道生态系统的完整性。     

Characterisation of Organic Matter and Carbon Cycling in Rehabilitated Lignite-rich Mine Soils

Open-cast lignite mining in the Lusatian mining district resulted in rehabilitated mine soils containing up to four organic matter types: (1) recent plant litter, (2) lignite deposited by mining activity, (3) carbonaceous ash particles deposited during amelioration of the lignite-containing parent substrate and (4) airborne carbonaceous particles deposited during contamination. The influence of lignite-derived carbon types on the organic matter development and their role in the soil carbon cycle was unknown. This paper presents the findings obtained during a six year project concerning the impact of lignite on soil organic matter composition and the biogeochemical functioning of the ecosystem. The organic matter development after rehabilitation was followed in a chronosequence of rehabilitated mine soils afforested in 1966, 1981 and 1987. A differentiation of the organic matter types and an evaluation of their role within the ecosystem was achieved by the use of ¹⁴C activity measurements, ¹³C CPMAS NMR spectroscopy and wet chemical analysis of plant litter compounds. The results showed that the amount and degree of decomposition of the recent organic matter derived from plant material of the 30 year old mine soil was similar to natural uncontaminated forest soil which suggests complete rehabilitation of the ecosystem. The decomposition and humification processes were not influenced by the presence of lignite. On the other hand it was shown that lignite, which was thought to be recalcitrant because of its chemical structure, was part of the carbon cycle in these soils. This demonstrates the need to elucidate further the stabilisation mechanisms of organic matter in soils.     

塔河流域森林生态建设存在的主要问题及其策略

随着时间的推移,塔河流域生态环境恶化的现实已被越来越多的人们所认识。造成塔河中下游森林退化,生态环境恶化的主要原因除自然因素外,主要是人为不合理的社会经济活动的影响。目前塔河流域森林生态建设存在的主要问题有:第一水资源不合理的利用成为沙漠化和次生盐渍化的主要原因;第二传统的生活方式和掠夺式的生产方式使生态环境遭到破坏;第三耕地的增加,满足不了人口发展的需求,大面积的无序开荒,导致生态环境进一步恶化。为塔河中下游林地植被恢复、建设及防止退化的策略有:塔河中下游生态工程治理的原则;塔河中下游生态环境治理的基本思路;塔河流域生态环境治理的主要对策,合理利用水资源,坚持生产、生态建设并重,确保生态用水;要积极调整农村产业结构,正确处理林业和农业、林业和牧业以及林业和工业发展的关系;大力发展农村能源建设,从根本上解决农村薪材和生态保护之间矛盾;大力保护、恢复、发展荒漠林草植被,大力发展绿洲林业,建立荒漠生态环境和绿洲共同支撑的稳定的生态体系;积极实施林业生态工程和林果工程,建立较为完备和发达的生态和产业体系。     

塔里木河下游绿色走廊的生态问题与治理对策

从经济、军事和生态三个角度分析了保护塔里木河下游绿色走廊的必要性,概括了该区域的生态环境现状,即河水矿化度升高、地下水位下降从而导致植被的退化和沙漠化加剧的问题。最后提出了以水资源合理利用为前提和中心的治理对策。