超声波提取西兰花中叶黄素的工艺研究

以西兰花真空冻干粉为原料,探明了超声波强化四氢呋喃提取叶黄素的工艺条件。在确定适合提取料液比后,采用正交试验研究了超声波功率、提取温度、超声作用时间对提取量的影响。结果显示,较理想的提取条件为：超声波功率400W、提取温度40℃、超声波作用时间10min。在此条件下,叶黄素提取量最高。通过方差分析可知,温度影响达到0．05的显著水平。     

Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties

In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles (NPs) in green chemistry method. The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV–Vis spectrophotometer, Field Emission Scanning Electron Microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV–Vis color intensity of the nanoparticles was shown at 540 and 450 nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanoparticles were seen in spherical shapes. The average spherical size of the particles was observed in 24–38 nm for gold and 30–45 nm for silver NPs. X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally, the functional metabolites were identified by the Fourier Transform Infrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes (sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50 μg/mL respectively. In addition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens.     

A comparison of two stomatal conductance models for ozone flux modelling using data from two Brassica species

In this study we tested and compared a multiplicative stomatal model and a coupled semi-empirical stomatal-photosynthesis model in their ability to predict stomatal conductance to ozone (g_st) using leaf-level data from oilseed rape (Brassica napus L.) and broccoli (Brassica oleracea L. var. italica Plenck). For oilseed rape, the multiplicative model and the coupled model were able to explain 72% and 73% of the observed g_st variance, respectively. For broccoli, the models were able to explain 53% and 51% of the observed g_st variance, respectively. These results support the coupled semi-empirical stomatal-photosynthesis model as a valid alternative to the multiplicative stomatal model for O₃ flux modelling, in terms of predictive performance.     

Hydrolytic biodegradation of chlorothalonil in the soil and in cabbage crops

Broccoli and Chinese cabbage crops were treated at planting by pouring an emulsion of the fungicide chlorothalonil around the stem of the plant. During culture, chlorothalonil was biodegraded in soil into l,3‐dicarbamoyl‐2,4,5,6‐tetrachlorobenzene (compound 1), l,3‐dicyano‐4‐hydroxy‐2,5,6‐trichlorobenzene (compound 2), and l‐carbamoyl‐3‐cyano‐4‐hydroxy‐2,5,6‐trichlorobenzene (compound 3). Compounds 1 and 2 were the major metabolites in soil. In the harvested broccoli (in the flower) and Chinese cabbages (the leaves), the concentrations of chlorothalonil and of compounds 1 and 2 were lower respectively than 0.1, 0.1 and 0.5mg/kg fresh weight.     

Intracellular biosynthesis of Au and Ag nanoparticles using ethanolic extract of Brassica oleracea L. and studies on their physicochemical and biological properties

In this present study, we reported broccoli (Brassica oleracea L.) as a potential candidate for the synthesis of gold and silver nanoparticles (NPs) in green chemistry method. The synthesized metal nanoparticles are evaluated their antimicrobial efficacy against different human pathogenic organisms. The physico-chemical properties of gold nanoparticles were analyzed using different analytical techniques such as a UV-Vis spectrophotometer, Field Emission Scanning Electron Microscopy, energy dispersive X-ray spectroscopy, X-ray diffraction and a Fourier Transform Infrared spectrophotometer. In addition, gold and silver NP antimicrobial efficacy was checked by disc diffusion assay. UV-Vis color intensity of the nanoparticles was shown at 540 and 450 nm for gold and silver nanoparticles respectively. Higher magnification of the Field Emission Scanning Electron Microscopy image shows the variable morphology of the gold nanoparticles such as spherical, rod and triangular shapes and silver nanoparticles were seen in spherical shapes. The average spherical size of the particles was observed in 24- 38 nm for gold and 30-45 nm for silver NPs. X-ray diffraction pattern confirmed the presence of gold nanoparticles and silver nanoparticles which were crystalline in nature. Additionally, the functionalmetabolites were identified by the Fourier TransformInfrared spectroscopy. IR spectra revealed phenols, alcohols, aldehydes (sugar moieties), vitamins and proteins are present in the broccoli extract which are accountable to synthesize the nanoparticles. The synthesized gold and silver NPs inhibited the growth of the tested bacterial and fungal pathogens at the concentration of 50 μg/mL respectively. In addition, broccoli mediated gold and silver nanoparticles have shown potent antimicrobial activity against human pathogens. © 2015 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences.