化学发光流动注射法测定雨水中过氧化氢

用化学发光流动注射分析装置,将16孔阀连接成异步双流路,以过氧化氢-鲁米诺-钴(Ⅱ)为化学发光体系,建立了快速测定雨水中过氧化氢的方法,最低检测限为0.1ng/ml,在0.1—400ppb范围内有良好的线性关系,相对标准差为0.78—3.03％,每小时可分析480个以上的样品.     

Immunotoxicity of bisphenol a to Carassius auratus Lymphocytes and macrophages following in vitro exposure

Bisphenol A （BPA） is the monomer component of polycarbonate plastics and classified as an endocrine disrupting chemical （EDC）. The reproductive toxicity of BPA has been extensively studied in mammals; however, relatively little information is available on the immunotoxic responses of fish to BPA. In this study, we investigated the effects of BPA on the immune functions of lymphocytes and macrophages in Carassius auratus. The effects of BPA were compared with those of two natural steroid hormones, estradiol and hydrocortisone. Proliferation of the two types of cells in response to PHA was measured using colorimetric MTT assay. Macrophage respiratory burst stimulated by Con A was measured using chemiluminescence assay. Results showed that BPA （0.054-5.4 mg/L）, estradiol （0.0002-2.0 mg/L） and hydrocortisone （5-50 mg/L） significantly induced Carassius auratus lymphocyte proliferation while higher doses of hydrocortisone （500-5000 mg/L） appeared to be inhibitory. BPA （0.005-50 mg/L）, estradiol （0.005-800 mg/L） and hydrocortisone （0.005-500 mg/L） markedly enhanced macrophage proliferation, whereas higher doses of BPA （500-1000 mg/L） appeared to inhibit cell proliferation. Furthermore, higher dosage of BPA （50 mg/L） and hydrocortisone （50 and 500 mg/L） suppressed the macrophages respiratory burst while estradiol is stimulative all the doses tested （0.05-500 mg/L）. In conclusion, BPA could have immunotoxicity to Carassius auratus and functional changes of lymphocyte and macrophage in Carassius auratus may be different between low and high dosages.     

用化学发光法测定雨水中的氨

本文研究了用ＴＣＰＯ－Ｈ２Ｏ２发光体系对氨进行测定。结果表明，当ｐＨ＝１０．３，ＯＰＡ浓度为１０ｍｍｏｌ．ｌ＾－１，氨浓度在０－６ｍｇ．ｌ＾－１时，其浓度和发光强度有良好的线性关系（相关系数为０．９９８），本法的最低检出限为２ｎｇ．ｌ＾－１。可用于实际雨水样品的测定，测定结果还表明：本法具有灵敏度高，精度高，线性范围宽干扰少等优点，适用于大批量分析及连续自动监测分析。     

上海中心城区二氧化氮在线测量比对研究

对比了基于腔衰减相移光谱技术(CAPS)、化学荧光法(CL)和非相干宽带腔增强吸收光谱技术(IBBCEAS)3种不同方法在线测量NO_2浓度的仪器,并于2017年5月10日—6月10日采用3台仪器对上海中心城区大气环境NO_2进行监测,验证了IBBCEAS实验装置的稳定性、灵敏度、检测下限等关键特性,证明可以实现复杂环境下大气痕量气体高精度在线监测.IBBCEAS装置与其它两种仪器监测数据的一致性较好,监测过程中上海市中心NO_2日浓度变化范围为3~63 ppbv.各仪器测量结果之间的线性关系图表明,3种方式对NO_2浓度监测较为准确,偏差普遍在10 ppbv.根据早晚高峰车流量增加情况分析可以看出,NO_2高浓度污染主要来自于车辆排放.另外发现,受局地污染源的影响,NO_2浓度随时间推移而增加.     

The influence of some biological antioxid ants on the light emission from the oxytetracycline oxidation reaction

The effect of the Superoxide radical (O₂ ^–)‐, hydroxyl radical (HO')‐inhibitors, singlet oxygen (¹O₂)‐quenchers and catalase on the light emission during autoxidation reaction of oxytetracycline was measured. The influence of the ¹O₂‐quenchers was investigated quantitatively and the rate constants were established.     

活性氧的化学发光测定法

以作者近年来的研究结果为中心 ,综合地介绍过氧化氢 (H2 O2 )、单线态氧 ( 1O2 )、超氧阴离子自由基 ( .O-2 )以及羟自由基 ( .OH)等活性氧化学发光研究的最新动态 .侧重介绍活性氧的鲁米诺、光泽精、甲壳动物荧光素化学发光测定体系 ,比较了这几种方法的各自特点和应用情况     

测定双酚A的流动注射化学发光法研究

在鲁米诺-高碘酸钾化学发光体系中加入双酚A,可以抑制该体系的化学发光,据此建立了双酚A的流动注射化学发光监测分析新方法。该法的线性范围为6 0×10-6～1 0×10-3g L,检测限为1 3×10-7g L。方法可用于水体中双酚A的测定。     

流动注射-化学发光流通池芯片及其在环境污染物测定中的应用

1　IntroductionMiniaturizationofanalyticalsystemhasbeenpaidmuchattentiontointherecentyearsforitsobviousadvantages:fastanalysis,portability,reducedconsumptionofreagentsandimprovingper formance .Suchdeviceisso calledaslaboratory on a chip[1 ],whichistheinte…     

Organic Nitrogen in Precipitation Across the United Kingdom

The concentration of water-soluble organic nitrogen (WSON) in precipitation has been measured at seven sites across the United Kingdom, over a period of 1–2 years, using protocols developed in a pilot study. Samples were collected over 1–2 weeks in continuously open funnels made of stainless steel, draining to a glass bottle, and were preserved during and after collection by the addition of thymol. After chemical analysis, samples were excluded from the long-term average if they showed signs of contamination (significant concentrations of K⁺ or PO₄ ^3–). Two methods of measuring total dissolved N were used, persulphate oxidation and high-temperature chemiluminescence. The latter generally gave the larger values, and has been used to asses the organic component of dissolved N. The long-term data set confirms the original results from the pilot study - organic N contributes between 24 and 40% to the total annual wet deposition of dissolved N across the United Kingdom. The fraction of WSON was greatest at western sites, and was strongly correlated with ammonium concentrations. However, the seasonal pattern across all sites showed a late spring maximum for ammonium and nitrate, but a late summer maximum for WSON. The magnitude of the contribution of WSON to wet-deposited N has implications for the setting and assessment of critical loads for N deposition.     

化学发光法测定废水中痕量苯酚

本文研究了鲁米诺-H_2O_2(KOH,H_3BO_3)发光体系和苯酚对该体系发光的淬灭作用。发现苯酚浓度处于2.0×10~(-6)—2.0×10~(-4)mol·L~(-1)时,与淬灭后的相对发光强度有线性关系,本法最低检出限为0.2mg·L~(-1)。用于工业废水中苯酚的测定,测定结果满意。