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Bunge Michael Khknen Mika A. Rmisch Winfried Opel Matthias Vogler Susanne Walkow Fred Salkinoja-Salonen Mirja Lechner Ute 《Environmental science and pollution research international》2007,14(1):3-10
Background, Aims and Scope Sediments of the Spittelwasser creek are highly polluted with organic compounds and heavy metals due to the discharge of untreated
waste waters from the industrial region of Bitterfeld-Wolfen, Germany over the course of more than one century. However, relatively
few data have been published about the chloroorganic contamination of the sediment. This paper reports on the content of different
(chloro)organic compounds with special emphasis on polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/F), and chlorobenzenes. Existing concepts for the remediation of Spittelwasser sediment include
the investigation of natural attenuation processes, which largely depend on the presence of an intact microbial food web.
In order to gain more insight in terms of biological activity, we analyzed the capacity of sediment microflora to degrade
organic matter by measuring the activities of extracellular hydrolytic enzymes involved in the biogeochemical cycling of carbon,
nitrogen, phosphorus and sulfur. Furthermore, the detection of physiologically active bacteria in the sediment, particularly
of those known for their capability to reductively dehalogenate organochlorine compounds, illustrates the potential for intrinsic
bioremediation processes.
Methods PCDD/F and chlorobenzenes were analyzed by gas chromatography(GC)/mass spectrometry and GC/flame ionization detection, respectively.
The activities of hydrolytic enzymes were determined from freshly sampled sediment layers using 4-methylumbelliferyl (MUF)
or 7-amino-4-methylcoumarin-conjugated model compounds and kinetic fluorescence measurements. Physiologically active bacteria
from different sediment layers were microscopically visualized by fluorescence in situ hybridization (FISH). Specific bacteria were identified by 16S rRNA gene amplification and sequencing.
Results and Discussion The PCDD/F congener profile was dominated by dibenzofurans. In addition, the presence of specific tetra and pentachlorinated
dibenzofurans supported the assumption that extensive magnesium production was one possible source for the high contamination.
A range of other chloroorganic compounds, including several isomers of chlorobenzenes, hexachlorocyclohexane and 1,1,1-trichloro-2,2-bis
(p-chloro-phenyl)ethane (DDT), was present in the sediment. Activities of extracellular hydrolytic enzymes showed a strong
decrease in those sediment layers that were characterized by high contents of absorbable organic halogen (AOX), indicating
disturbed organic matter decay. Interestingly, an abnormal increase of cellulolytic enzyme activities below the organochlorine-rich
layers was observed, possibly caused by residual cellulose from discharges of sulfite pulping wastes. FISH revealed physiologically
active bacteria in most sediment layers from the surface down to the depth of about 60 cm, including members of Desulfitobacterium (D.) and Sulfurospirillum. The presence of D. dehalogenans was confirmed by its partial 16S rRNA gene sequence.
Conclusions Results of chemical sediment analyses demonstrated high loads of organochlorine compounds, particularly of PCDD/F. Several
years after stopping the waste water discharge to Spittelwasser creek, this sediment remains a main source for pollution of
the downstream river system by way of the ongoing mobilization of sediment during high floods. As indicated by our enzyme
activity measurements, the decomposition potential for organic matter is low in organochlorine-rich sediment layers. In contrast,
the comparably higher enzyme activities in less organochlorine-polluted sediment layers as well as the presence of physiologically
active bacteria suggest a considerable potential for natural attenuation.
Recommendations and Perspectives From our data we strongly recommend to explore the degradative capacity of sediment microorganisms and the limits for in situ activity towards specific sediment pollutants in more detail. This will give a sound basis for the integration of bioremediation
approaches into general concepts to reduce the risk that permanently radiates from this highly contaminated sediment.
Submission Editor: Dr. Henner Hollert (Henner.Hollert@urz.uniheidelberg.de) 相似文献
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湿法烟气脱硫的腐蚀机理及防腐技术 总被引:4,自引:0,他引:4
综述了湿法烟气脱硫的腐蚀与防腐现状,分析了腐蚀环境及腐蚀机理,并概括总结了湿法烟气脱硫工艺系统中材料防腐技术和工艺防腐技术,对此法的现场实际应用有一定的指导意义。 相似文献
5.
测定环境空气中微量氟的方法一般采用滤膜法,但此法工作量大,针对这种情况,本文作两点改进:一是将采集的两张滤膜同时测定;二是将测定过程中的搅拌时间缩短,这样不影响测定结果的准确性,而且降低了工作量。 相似文献
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介绍了系统安全评价的AHP方法,叙述了该方法的基本步骤,并以某公司热处理分厂评忏为实例,证明了该方法的有效性和实用性。 相似文献
7.
通过对变流量冷冻水系统的分析指出,在运用平衡调温调湿方式的空调系统中,应用变流量冷冻水系统可以较大地节约能源和投资;并提出了实现这种应用的方法。 相似文献
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复杂矿井通风系统角联风路自动识别方法的研究 总被引:4,自引:0,他引:4
角联风路的识别与稳定性分析是矿井通风系统稳定性与可靠性分析理论中的核心内容之一。笔者对通风系统角联风路进行了深入的研究;分析了通路法的局限性,简化了角联风路的数学模型,提出了快速、准确、灵活的角联风路自动识别的新方法——节点位置法;编写了角联风路自动识别软件,并应用现场数据进行测试,将其结果与通路法的结果进行了比较分析;证明了节点位置法是切实可行的。 相似文献
10.
The recreational-use value of hiking in the Bellenden Ker National Park, Australia has been estimated using a zonal travel cost model. Multiple destination visitors have been accounted for by converting visitors’ own ordinal ranking of the various sites visited to numerical weights, using an expected-value approach. The value of hiking and camping in this national park was found to be $AUS 250,825 per year, or $AUS 144,45 per visitor per year, which is similar to findings from other studies valuing recreational benefits. The management of the park can use these estimates when considering the introduction of a system of user pays fees. In addition, they might be important when decisions need to be made about the allocation of resources for maintenance or upgrade of tracks and facilities. 相似文献