Poisoned plusiines: toxicity of milkweed latex and cardenolides to some generalist caterpillars

Summary. Larvae of Trichoplusia ni Hübner (Lepidoptera: Noctuidae) that ingested latex from Asclepias curassavica L. (Asclepiadaceae) often regurgitated and convulsed with spasms before becoming immobile and unresponsive. Some larvae required over a day to recover sufficiently to feed. Latices from four other plant species in three families were all deterrent, but none caused detectable poisoning. The toxicity of A. curassavica latex was evidently due to cardenolides because pure cardenolides had similar effects when ingested by T. ni. Other species of noctuid caterpillars (Rachiplusia ou Guenée, Anagrapha falcifera Kirby, and Autographa precationis Guenée) sometimes also suffered spasms and temporary immobility when fed A. curassavica latex. A more distantly related noctuid, Spodoptera ornithogalli, was deterred by the latex, but showed no overt physiological responses at the dosage tested. T. ni larvae failed to develop on intact leaves of A. curassavica, on leaves with latex canals deactivated by midrib severance, and on excised leaves. Similarly, larvae reared on excised A. syriaca L. leaves to the final instar died when transferred to A. curassavica leaves with either intact or severed midribs. The final instar larvae sometimes suffered from spasms and immobility even when confined on leaves with depressurized canal systems. Evidently, cardenolides stored outside the latex system suffice to poison larvae. We conclude that cardenolides in A. curassavica have potent physiological effects on some generalist caterpillars and that the presence of these compounds both inside and outside laticifers effectively protects the plant. Received 30 June 1999; accepted 18 October 1999     

Glues or poisons: which triggers vein cutting by monarch caterpillars?

Summary. Late instar larvae of the monarch butterfly (Danaus plexippus L., Lepidoptera: Nymphalidae) deactivate the latex defense of milkweeds by chewing a furrow in the leaf midrib or petiole. The larva then feeds beyond the cut where latex outflow is minimal. If a larva does encounter latex exudate during feeding, it often returns to its initial cut to damage the midrib or petiole more extensively before resuming feeding. We used this response to latex as an assay for testing what cue triggers vein cutting. A sticky solution of polyethylene glycol and a mixture of cardenolides both were ineffective; drops applied to the mouthparts of feeding monarchs failed to elicit renewed vein cutting. Activity resided instead within a methanol extract of the supernatant obtained from centrifuged latex of the milkweed, Asclepias curassavica L. (Asclepiadaceae). Treatment with proteinase K did not eliminate activity documenting that the active compound is unlikely to be proteinaceous. Our results indicate that latex adhesiveness and low polarity cardenolides are relatively unimportant releasers of vein cutting. We propose that milkweeds contain noncardenolide stimulants of vein cutting; these compounds presumably serve a defensive role for milkweeds. Over 50 species of insects are known to employ vein-cutting before feeding on plants with canal-borne exudates; most of these species are dietary specialists like the monarch. Our behavioral assay provides a novel approach for identifying ecologically-significant compounds in the exudates of their diverse host plants.