实验性糖尿病新西兰兔主要器官组织的病理学观察

将雄性新西兰兔随机分为:基础饲料组(即对照组:n=15)和高糖高脂饲料组(即实验组:n=15,喂食10％猪油,37％白蔗糖混合53％的基础饲料).共观察12周.于12周实验结束时,全部处死动物,取动物心脏、肝脏、胰腺、肾脏,用1O％福尔马林液固定,常规石蜡包埋切片,H、E染色,光学显微镜下观察并照相.结果表明:动物心肌细胞肥大,并出现灶性坏死,淋巴细胞浸润,心肌细胞间成纤维细胞增生和毛细血管增生,胶原纤维增加;肝细胞有明显脂肪变性,细胞肿胀;肾脏表现有肾小球球囊增大,细胞增多,毛细血管壁增厚,肾小管上皮细胞胞体增大;胰腺组织表现为胰岛分布稀疏,数量减少,胰岛萎缩,胰岛细胞数量减少,成纤维细胞增生.     

Screening for acute ocular toxicity of commercialized products of cartap,an insecticide,and correlation to blood concentrations in rabbits

Cartap is an insecticide known to produce contraction and damage to mouse and rabbit diaphragms, and plays a pivotal role in respiratory paralysis and mortality in rabbits. The aim of this study was conducted to screen for the toxic properties and blood concentrations of commercialized cartap products in New Zealand White (NZW) rabbits. Five granular formulations (GRs) with different concentrations (4, 6, 6.5, 6.66 or 10%) and seven water-soluble powders (SPs, 50%) of commercialized cartap products were chosen to study toxicity following ocular instillation. Furthermore, ocular instillation (5, 12.5, 25 or 50 mg kg^?1) and oral administration (25 mg kg^?1) of the technical material (TC) cartap was also performed to compare blood concentration changes in rabbits. There was no death or eye irritation with all GR products; however, soluble powder (SP) products produced severe toxicity and mortality in rabbits. The blood concentration of TC cartap in rabbits started to increase from 2 min and reached peak levels at 7–9 min after ocular instillation in a dose-related pattern. For comparison of the blood distribution of cartap via different administration routes, oral exposure exhibited a higher absorption of cartap than ocular instillation. It was suggested that the commercialized GR products of cartap were safe but 50% SP products were highly toxic to rabbits following ocular exposure. This study provides valuable data to screen for potential acute toxicity of cartap to farmers and animals in the field.     

Effect of dietary Jatropha curcas seed meal on rabbits infested by adult ticks of Hyalomma marginatum marginatum

The objective of this study was to investigate the effectiveness of the oral administration of Jatropha curcas seed meal (JCSM) on serum biochemical parameters and histopathology of skin, liver, and kidney of rabbits experimentally infested by Hyalomma marginatum marginatum. Thirty healthy mixed-breed rabbits were randomly divided into five equal groups. The first group was kept as a control and fed soya bean meal. The remaining groups were fed diets containing 2.5%, 5%, 7.5%, or 10% JCSM. At the sixth week, each group was divided into two subgroups. Ticks were introduced to all members of one subgroup, the other subgroup being kept as control. Blood samples were collected and analyzed for biochemical parameters before (zero time) and after the treatment, on the 6th and 8th weeks. Histopathological studies were conducted 8 weeks after the treatment. The results revealed that JCSM could be used in the treatment of ectoparasites at levels of less than 10% in the diet without any serious effects on liver and kidney functions.     

Some bacteriological aspects of poultry litter used as feed supplement in rabbit diets

An investigation was carried out to determine the microbial load in poultry litter used as feed supplement in rabbit diets. Before heat treatment by deep stacking the poultry litter, samples of the litter were collected to assay for microbial load at the Microbiology Laboratory of the Rivers State University of Science and Technology, Port Harcourt, Nigeria. Results obtained showed that before heat-treating the poultry litter, Escherichia coli, Salmonella, Mycobacterium, Clostridium and Staphylococcus were isolated at 41, 37, 37, 41 and 42°C, respectively. The results obtained also indicate that no pathogen was isolated after heat treatment of between 40 and 55°C for 21 days. This study justifies the suitability of using poultry litter as a feed supplement.     

Effects of two organophosphorus insecticides on some biochemical constituents in the nervous system and liver of rabbits

Abstract

Male Baladi rabbits were acutely and sub‐chronically intoxicated with cyanofenphos and profenophos. The levels of cholesterol, triglycerides, B‐lipoproteins and total proteins were determined in the serum, brain, spinal cord and sciatic nerve of rabbits. Moreover, the activities of alkaline phosphatase, acid phosphatase and glutamic‐pyruvic transaminase were determined in the liver of the animals. The whole Studie revealed that the biochemical constituents were highly affected by the tested insecticides. Also, the liver function suffered from adverse effects of the tested insecticides.