白银矿区土壤重金属及可培养细菌重金属耐受性研究

对白银矿区土壤重金属进行分析研究,分离、筛选和鉴定了受污染土壤中的可培养细菌,并考察其对土壤中重金属胁迫的耐受性。结果表明：白银矿区王岘镇尾渣堆和东大沟河沟土壤重金属污染Pb＞Zn＞Cu;从污染最严重的土壤中分离、筛选出砖红色微杆菌（Microbacterium testaceum）、特基拉芽孢杆菌（Bacillus tequilensis）和解单端孢菌素微杆菌（Microbacterium trichothecenolyticum）3种细菌,其中菌株M1（Microbacterium testaceum）对Pb、Zn、Cu、Cd的胁迫具有明显的耐受性。     

Isolation of culturable mycobiota from agricultural soils and determination of tolerance to glyphosate of nontoxigenic Aspergillus section Flavi strains

Glyphosate-based herbicides are extensively used in Argentina's agricultural system to control undesirable weeds. This study was conducted to evaluate the culturable mycobiota [colony forming units (CFU) g^?1 and frequency of fungal genera or species] from an agricultural field exposed to pesticides. In addition, we evaluated the tolerance of A. oryzae and nontoxigenic A. flavus strains to high concentrations (100 to 500 mM – 17,000 to 84,500 ppm) of a glyphosate commercial formulation. The analysis of the mycobiota showed that the frequency of the main fungal genera varied according to the analyzed sampling period. Aspergillus spp. or Aspergillus section Flavi strains were isolated from 20 to 100% of the soil samples. Sterilia spp. were also observed throughout the sampling (50 to 100%). Aspergillus section Flavi tolerance assays showed that all of the tested strains were able to develop at the highest glyphosate concentration tested regardless of the water availability conditions. In general, significant reductions in growth rates were observed with increasing concentrations of the herbicide. However, a complete inhibition of fungal growth was not observed with the concentrations assayed. This study contributes to the knowledge of culturable mycobiota from agricultural soils exposed to pesticides and provides evidence on the effective growth ability of A. oryzae and nontoxigenic A. flavus strains exposed to high glyphosate concentrations in vitro.     

Changes in desert steppe soil culturable bacteria from northwestern China and correlation with physicochemical parameters

Seasonal variations effects on desert steppe soil culturable bacteria in northwestern China and physicochemical parameters were investigated. Soil temperature ranged from 5.37 to 23.73 °C. Moisture content varied from 3.22% to 7.62%. The pH was between 7.98 and 8.72. Soil nutrients were altered in different seasons. Total potassium (K) remained the same, but available K levels ranged from 101.29 to 227.55 mg/kg. The culturable bacterial counts in January, April, July and October were 0.13 × 10⁷, 4.09 × 10⁷, 5.33 × 10⁷ and 1.8 × 10⁷ colony forming units (cfu)/g, respectively. Bacterial populations were most abundant in surface layers and declined with increase of soil depth. Seventy-two bacterial strains were isolated and classified according to 27 genera of 5 phyla groups: Firmicutes, Actinobacteria, Alphaproteobacteria, Gammaproteobacteria and Bacteroidates. The bacterial diversities varied according to seasons. In spring, only Firmicutes and Actinobacteria were isolated. In summer, Firmicutes, Actinobacteria and Bacteroidetes were detected with Firmicutes the dominant species. In autumn and winter, Firmicutes, Actinobacteria, α-Proteobacteria and γ-Proteobacteria predominated. Our results indicated that climatic variations, vegetation coverage and soil physicochemical parameters are critical factors for maintaining culturable bacterial populations and diversity in the desert steppe. Soil moisture content and temperature exerted marked influence on bacterial quantities and diversities.     

Characterization and source analysis of indoor/outdoor culturable airborne bacteria in a municipal wastewater treatment plant

The potential health risks of airborne bacteria emission from a wastewater treatment process have been concerned. However, few studies have investigated the differences in community structure between indoor and outdoor bacteria. In this work, the characterization of airborne bacteria was studied in a municipal wastewater treatment plant in Beijing, China. Two indoor (i.e., fine screen room and sludge dewatering house) and two outdoor (i.e., aeration tank and control site) sampling sites were selected. An Andersen six-stage impactor was used for collecting culturable airborne bacteria in the air, and Illumina MiSeq sequencing was conducted to track the emission source of the culturable airborne bacteria. The results indicate that, compared with the outdoor aeration tank site, the concentrations of culturable airborne bacteria in the indoor fine screen room with poor ventilation were more than ten times higher and the particle size was about twice as large. The community structures of indoor and outdoor culturable airborne bacteria were obviously different. Enterobacteriaceae and opportunistic pathogens were detected in indoor culturable airborne bacteria, with wastewater and sludge dewatering machine identified as the primary sources. Conversely, Enterobacteriaceae and opportunistic pathogens were not detected in outdoor culturable airborne bacteria. Outdoor high wind speed might have resulted in rapid dilution and mixing of culturable airborne bacteria generated from the aeration tank with the ambient air. The results of the present research suggest that covering pollution sources, increasing ventilation rates, and using protective measures for personnel should be implemented to decrease the exposure risk to indoor culturable airborne bacteria.     

Communities of Wood-Attacking Fungi in the Region of Oil and Gas Production

The communities of xylotrophic fungi were studied in the northern and middle taiga subzones of western Siberia, in forests growing in the region of oil and gas production and exposed to various anthropogenic factors (emissions of petroleum gas flares, oil pollution, spills of mineralized oil-field water, etc.). Structural and functional rearrangements in these communities were revealed, and the prevalence of rot in the tree stand was assessed.