An investigation of methods for enriching trophoblast from maternal blood

Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method.     

生物传感器快速测定BOD在海洋监测中的应用

选用一株耐高渗透压的酵母菌种作为敏感材料可以实现BOD的快速测定。采用海藻酸钙包埋法和聚乙烯醇包埋法两种细胞固定化方法并进行了比较。就聚乙烯醇固定化微生物进行了渗稼压和重金属离子影响的研究。     

Production of laccase by Coriolus versicolor and its application in decolorization of dyestuffs: (Ⅱ) Decolorization of dyes by laccase containing fermentation broth with or without self-immobilized mycelia

The capability of decolorization for commercial dyes by Coriolus versicolur fermentation broth containing laccase with or without immobilized mycelium was evaluated.With cell-free fermentation broth containing laccase,high decolorization ratio was achieved for acid orange 7,but not for the other dyes concerned.The immobilized mycelium was proved to be more efficient than the cell-free system.All the four dyestuffs studied were found being decolourized with certain extent by immobilized mycelium.The repeated-batch decolorization was carried out with satisfactory results.The experimental data showed that the continuous decolorization of wastewater from a printing and dyeing industry was possible by using self-immobilized C.Versicolor.     

包埋法固定化细胞技术及其在水处理中的应用研究

固定化细胞技术(主要为包埋技术),由于方法简单、条件温和、较高的稳定性及细胞容量,在废水处理中应用广泛.本文主要介绍了近年来固定化细胞技术在污水深度处理中的应用研究现状和发展前景,并对固定化细胞技术在污水深度处理过程中的影响因素进行简要论述.     

镉胁迫下大豆中镉的分布状况及其籽粒品质

溶液培养中0.5μmol·L-1镉胁迫浓度下,大豆表现出轻微受害症状,籽粒减产25 . 7%,而籽粒中粗脂肪和粗蛋白含量变化不大.镉在大豆中的积累分布状况为根>叶>籽>油,比例为32.100:1.690:1.000:0.003.大豆籽粒含镉4.89mg/kg,超过了国家环境标准规定的最高容许量.而大豆粗脂肪中含镉仅0.015mg/kg,远低于国家食品环境卫生标准.豆粕中含镉6.17mg/kg,表明大豆籽粒中的镉主要存在于粗蛋白和淀粉中.     

共生细菌SB1降解聚乙烯醇的研究Ⅱ．共生细菌SB1聚乙烯醇氧化酶的产生、纯

共生细菌ＳＢ１接种在０．３％聚乙烯醇培养堪中，３０℃摇床振荡培养或发酵罐通气培养时，ＰＶＡ氧化酶产生的高峰在４８ｈ或４０ｈ。离心收集的上清液采用４０％－５０％饱和度的硫酸铵分级沉淀ＤＥＡＤ－Ｓｅｐｈａｄｅｘ离子交换柱和Ｃｉｂａｃｒｏｎ蓝Ｓ－Ｓｅｐｈａｒｏｓｅ４Ｂ亲和柱层析等纯化步骤。ＰＶＡ氧化酶收得率３０％，比活力提高３０倍。     

固定化微生物技术在环境工程中的应用

固定化微生物技术是一种新技术，与普通生物处理法相比有许多优点，对固定化微生物技术、微生物的固定化方法、固定化栽体及固定化技术在环境工程中的应用和研究进展状况进行了综述，并对其发展问题做了探讨。     

浅谈环境信息公开与环境管理

环境信息公开是通过社区和公众的舆论，使环境行为主体产生改善其环境行为的压力，从而达到环境保护的目的。环境信息是环境管理和环境保护的基础，环境信息公开能够有效地加强环境管理的公众参与和监督，促进政府重视环境质量的改善，促使污染者加强污染防治，改善其环境行为。本文将对环境信息与环境管理的关系与作用进行阐述。     

Production of laccase by Coriolus versicolor and its application in decolorization of dyestuffs : (Ⅰ) Production of laccase by batch and repeated-batch processes

The production of laccase by Coriolus versicolor was studied.The effect of cultivation on laccase production by Coriolus versicolor was examined to obtain optimal medium and cultivation conditions.Both batch and repeated-batch processes were performed for laccase production.In repeated-batch fermentation with self-immobilized mycelia, total of 14 cycles were performed with laccase activity in the range between 3.4 and 14.8U/ml.     

壳聚糖/PVA微粒上Cu2+的吸附平衡与动力学

采用粒径＜200μm、在酸碱介质中溶胀率小且能稳定存在的壳聚糖/PVA微粒对Cu2+的吸附进行了研究,结果表明,在pH 6,室温条件下,其饱合吸附量达56.0mg/g,随温度升高,吸附量降低.通过计算不同温度下各热力学参数△G0、△H0和△S0,证实该吸附为自发的放热过程.对实验数据运用相关数学模型拟合,显示等温吸附平衡符合Langmuir模型,吸附过程动力学更适合二级反应,被吸附在壳聚糖/PVA微粒上的Cu2+,可被0.01mo/L的EDTS或HNO3溶液快速地脱附出来,其洗脱率分别为85.3%和65.9%.