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排序方式: 共有318条查询结果,搜索用时 15 毫秒
1.
Dr Marianne Johansen Marian Knight Edward J. Maher Kim Smith Ian L. Sargent 《黑龙江环境通报》1995,15(10):921-931
Trophoblast deportation is known to occur in normal human pregnancy, but it is not yet clear whether these cells routinely enter the maternal peripheral circulation and are available as a source of fetal DNA for non-invasive prenatal diagnosis of genetic disorders. To resolve this issue requires an efficient method of enriching trophoblast from maternal blood combined with a means to confirm its identity. Five different techniques were tested on ten retroplacental blood samples to determine the most sensitive and operator-efficient method. Lysis of red cells alone gave the best recovery of trophoblast but had to be discounted, together with Ficoll density gradient centrifugation, due to the very low purity and the excessive time required. Fluorescence-activated cell sorting (FACS) of pre-enriched trophoblast resulted in the lowest recovery rate (8 per cent) despite a 3250-fold enrichment and a very high purity. Immunomagnetic beads (Dynabeads) coated with anti-CD 16 antibody proved to be the best method for the subsequent immunocytochemical characterization of deported trophoblast. However, IO beads coated with anti-CD45 antibody may be more useful for isolating trophoblast for prenatal diagnosis due to the high purity, enrichment (32-fold), and recovery rate (78 per cent) obtained with this method. 相似文献
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The capability of decolorization for commercial dyes by Coriolus versicolur fermentation broth containing laccase with or without immobilized mycelium was evaluated.With cell-free fermentation broth containing laccase,high decolorization ratio was achieved for acid orange 7,but not for the other dyes concerned.The immobilized mycelium was proved to be more efficient than the cell-free system.All the four dyestuffs studied were found being decolourized with certain extent by immobilized mycelium.The repeated-batch decolorization was carried out with satisfactory results.The experimental data showed that the continuous decolorization of wastewater from a printing and dyeing industry was possible by using self-immobilized C.Versicolor. 相似文献
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镉胁迫下大豆中镉的分布状况及其籽粒品质 总被引:18,自引:1,他引:18
溶液培养中0.5μmol·L-1镉胁迫浓度下,大豆表现出轻微受害症状,籽粒减产25 . 7%,而籽粒中粗脂肪和粗蛋白含量变化不大.镉在大豆中的积累分布状况为根>叶>籽>油,比例为32.100:1.690:1.000:0.003.大豆籽粒含镉4.89mg/kg,超过了国家环境标准规定的最高容许量.而大豆粗脂肪中含镉仅0.015mg/kg,远低于国家食品环境卫生标准.豆粕中含镉6.17mg/kg,表明大豆籽粒中的镉主要存在于粗蛋白和淀粉中. 相似文献
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固定化微生物技术在环境工程中的应用 总被引:4,自引:0,他引:4
固定化微生物技术是一种新技术,与普通生物处理法相比有许多优点,对固定化微生物技术、微生物的固定化方法、固定化栽体及固定化技术在环境工程中的应用和研究进展状况进行了综述,并对其发展问题做了探讨。 相似文献
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浅谈环境信息公开与环境管理 总被引:1,自引:0,他引:1
环境信息公开是通过社区和公众的舆论,使环境行为主体产生改善其环境行为的压力,从而达到环境保护的目的。环境信息是环境管理和环境保护的基础,环境信息公开能够有效地加强环境管理的公众参与和监督,促进政府重视环境质量的改善,促使污染者加强污染防治,改善其环境行为。本文将对环境信息与环境管理的关系与作用进行阐述。 相似文献
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The production of laccase by Coriolus versicolor was studied.The effect of cultivation on laccase production by Coriolus versicolor was examined to obtain optimal medium and cultivation conditions.Both batch and repeated-batch processes were performed for laccase production.In repeated-batch fermentation with self-immobilized mycelia, total of 14 cycles were performed with laccase activity in the range between 3.4 and 14.8U/ml. 相似文献
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饮用水反硝化脱氮方法研究 总被引:2,自引:0,他引:2
为去除饮用水中的硝酸盐氮(NO3ˉ-N),采用上流式厌氧污泥床(UASB)反应器和固定化微生物进行异养反硝化;自制固定化反硝化菌涂层电极和生物电化学脱氮装置,并用于自养反硝化。试验结果表明。若以甲醇为碳源。当碳氮比(C/N)≥1.o时,室温下经UASB处理4h,NO3ˉ-N去除率为97.7%;若以乙酸为碳源。当C/N≥1.0时,30C下经固定化微生物处理6h,N03ˉ-N去除率为98.6%;在无外加碳源的条件下处理东湖现场水样,30C下经60h后。N03ˉ-N去除率达93.5%。生物电化学脱氮装置可迅速建立自养反硝化菌所需的厌氧环境,水样在室温下经72h处理,脱氮率达96.3%。 相似文献
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