类二 多氯联苯对赤子爱胜蚓生理活性的影响

通过人工土壤法,研究0.1, 1, 10, 100, 1000μg/kg类二 多氯联苯暴露浓度对赤子爱胜蚓 (Eisenia fetida)抗氧化酶(SOD酶、CAT酶和GST酶)、抗氧化分子(GSH)、氧化损伤物(MDA)等生理活性指标在第2, 7, 14, 28d时的影响.结果显示,类二 多氯联苯刺激赤子爱胜蚓生理活性显著增加(P<0.05),可诱导蚯蚓抗氧化系统应答启动;不同暴露时间下,受试物浓度与蚯蚓生理活性的相关性分析显示,蚯蚓不同生理活性指标对受试物的最佳响应时间不同, Cu-Zn SOD响应最早(7d), SOD, CAT, GSH, MDA次之(14d), GST响应最迟(28d);在最佳响应时间下,受试物暴露浓度对数与生理活性值对数均存在显著的剂量-效应关系(P <0.001)

Effect of dioxin-like PCBs on physiological activities of earthworms (Eisenia fetida)

Using artificial soil test,physiological activities in earthworms (Eisenia foetida),which included superoxide dismutase(SOD,Cu-Zn SOD),catalase(CAT)and gnlathione-S-transferase(OST),and other biochemical activities,such as GSH and MDA,exposedto dioxin-like PCBs at the levels of 0.1,1,10,100,1000μg/kg,were observed after 2,7,14 and 28d exposure.Dioxin-like PCBs could significantly increase SOD,Cu-Zn SOD,CAT,GST activities and GSH and MDA levels(P<0.05),and induce starting of antioxidant systems in the earthworms.The relationships results showed that the best response times of various physiological activities in earthworms to dioxin-like PCBs were different.The best response time of Cu-Zn SOD activities was 7 days.whereas that of GST activities was 28 days.The best response times of others physioiogical activities were all 14 days.During the best response times,there were significantly dose-response relationships between the dioxin-like concentrations logarithms and physiological activities logarithms (P<0.001).