| 海洋细菌B3B产河豚毒素特性的鉴定及其发酵培养基优化 |
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| 引用本文： | 范延辉,胡江春,王书锦.海洋细菌B3B产河豚毒素特性的鉴定及其发酵培养基优化[J].应用与环境生物学报,2007,13(3):361-364. |
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| 作者姓名： | 范延辉 胡江春 王书锦 |
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| 作者单位： | 1. 中国科学院沈阳应用生态研究所,沈阳,110016;中国科学院研究生院,北京,100039
2. 中国科学院沈阳应用生态研究所,沈阳,110016 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 从我国渤海红鳍东方豚(Fugu rubripes)的卵巢中分离到了一株海洋细菌B3B,对其发酵产物进行了分离和精制,通过小鼠试验、高效液相色谱试验及质谱分析,认定其发酵产物中含有河豚毒素(tetrodotoxin,TTX).采用均匀设计回归分析及优化系统对其培养基组分做了优化,获得了较理想的发酵培养基,使TTX的产量提高了128.7%.图2表2参21
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| 关 键 词： | 河豚毒素 红鳍东方豚 均匀设计 小鼠单位 |
| 收稿时间： | 2005-12-21 |
| 修稿时间： | 2005-12-212006-03-28 |
Optimization of Culture Medium for a New Tetrodotoxin-producing Marine Bacterium B3B by Uniform Design |
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| FAN Yanhui,HU Jiangchun,WANG Shujin.Optimization of Culture Medium for a New Tetrodotoxin-producing Marine Bacterium B3B by Uniform Design[J].Chinese Journal of Applied and Environmental Biology,2007,13(3):361-364. |
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| Authors: | FAN Yanhui HU Jiangchun WANG Shujin |
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| Institution: | 1 Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China ; 2 Graduate University of Chinese Academy of Sciences, Beijing 100039, China |
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| Abstract: | A new tetrodotoxin-producing marine bacterium, B3B was isolated from the ovary of puffer fish Fugu rubripes collected from the Bohai Sea, and the tetrodotoxin (TTX)was identified by high-performance liquid chromatography (HPLC), mass spectrometry (MS) and mouse toxicity assay. UROS software was used to choose the optimum medium constituents. The TTX yield from the optimized medium was increased by 128.7%, compared to the original medium. Fig 2, Tab 2, Ref 21 |
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| Keywords: | tetrodotoxin Fugu rubripes uniform design mouse unit |
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