| 丙烯酰胺降解细菌的质粒检出及生长特性研究 |
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| 引用本文： | 边才苗,李钧敏,邵红. 丙烯酰胺降解细菌的质粒检出及生长特性研究[J]. 环境科学与技术, 2004, 27(1): 9-10,24 |
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| 作者姓名： | 边才苗 李钧敏 邵红 |
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| 作者单位： | 浙江台州学院生物系,临海,317000;浙江台州学院生物系,临海,317000;浙江台州学院生物系,临海,317000 |
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| 基金项目： | 浙江省台州学院校立项目(2002XL18)。 |
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| 摘 要： | 从化工厂的活性污泥中筛选出具有降解丙烯酰胺能力的细菌12株,经鉴定均为不动杆菌属。碱裂解法抽提显示质粒检出率为75%,并在质粒量上有所差异。在实验室条件下,这些菌株中对丙烯酰胺的最大耐受力可达40mg/mL;12株菌株都对氨苄青霉素有抗性,而对链霉素、卡那霉素、庆大霉素及氯霉素无抗性;在以丙烯酰胺为唯一碳源的无机盐培养基中,菌株DAW01在30℃,108γ/min旋转式摇床振荡培养6d后,对丙烯酰胺降解能力可达45.01%。
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| 关 键 词： | 丙烯酰胺 降解细菌 质粒 生长特性 |
| 文章编号： | 1003-6504(2004)01-0009-03 |
Plasmids in Acrylamide Degrading Bacteria:Detection and Study of Their Growth Properties |
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| BIAN Cai-miao,LI Jun-min,SHAO Hong. Plasmids in Acrylamide Degrading Bacteria:Detection and Study of Their Growth Properties[J]. Environmental Science and Technology, 2004, 27(1): 9-10,24 |
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| Authors: | BIAN Cai-miao LI Jun-min SHAO Hong |
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| Abstract: | Acrylamide degrading bacteria were screened from the activated sludge of a chemical plant and identified as Acinetobacteria. Results of the laboratory tests show the strains’ maximum tolerance to acrylamide could reach 40mg/mL and all of the strains have resistance to Ampicilin sodium. Shaking cultured at 30℃ and 108 γ/min for 6 days in mineral medium (MM) and with acryamide as an exclusive carbohydrate, the strain DAW01 displayed relatively higher degrading ability. |
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| Keywords: | acrylamide degrading bacteria plasmid growing properties |
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