土壤正构烷烃微生物降解变化实验分析

在石油烃微生物降解的最佳条件下,利用色谱-质谱联用技术分析测定了石油烃中正构烷烃的降解规律.结果表明,正构烷烃的降解最为明显;正构烷烃的降解随着碳数的增加其降解速度逐渐减小;其中C23烷的降解速度与石油烃整体降解速度相当,碳数小于23的正构烷烃降解速度大于石油烃降解速度,碳数大于23的正构烷烃降解速度小于石油烃降解速度;C16～C25的正构烷烃在0～6*!d内降解速度最快,在7～14 d内达到最低值,之后有逐渐上升的趋势;整个正构烷烃的降解符合二级反应动力学规律. 

Experimental Analysis of N-Alkanes Degradation by Microorganisms in Soil

Under the optimal conditions for degradation of petroleum hydrocarbon by microorganisms,GC\|MS technique was used to monitor and analyze the degradation patterns of n\|alkanes in soil.The results showed that n\|alkanes have the fastest degradation rate among petroleum hydrocarbons;heavier n\|alkanes have slower degradation rate;tricosane shares the same degradation rate with the total petroleum hydrocarbons in the soil;the degradation rate of alkanes with carbon number less than 23 is greater than that of total petroleum hydrocarbons;and the degradation rate of alkanes with carbon number greater than 23 is less than that of total petroleum hydrocarbons.The degradation rates of C16～C25 are biggest in the period from the beginning to the 6th day,reach the minimum from the 7th to 14th day,and have the increasing trend afterwards.The degradation of the total n\|alkanes follows with the second order reaction kinetics law.