| 两种实验设计研究DES和EV对MCF-7细胞增殖的联合作用 |
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| 引用本文： | 刘倩,雷炳莉,安静,尚羽,钟玉芳,康佳,文育.两种实验设计研究DES和EV对MCF-7细胞增殖的联合作用[J].环境科学,2013,34(8):3303-3308. |
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| 作者姓名： | 刘倩 雷炳莉 安静 尚羽 钟玉芳 康佳 文育 |
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| 作者单位： | 上海大学环境与化学工程学院,环境污染与健康研究所,上海200444 |
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| 基金项目： | 上海市重点学科项目(S30109); 上海市优秀青年基金项目(shu10040) |
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| 摘 要： | 研究了己烯雌酚(DES)和戊酸雌二醇(EV)对MCF-7细胞增殖的单一毒性和等浓度配比下的联合毒性作用,用相加指数法进行了联合毒性作用类型的判断,同时进行了联合毒性作用的3×3析因实验设计研究结果作为对比.结果表明,EV的24、48和72 h对MCF-7细胞增殖率的EC50值分别为6.02、0.40和0.33 nmol·L-1,DES的24、48和72 h的EC50值分别为5.90、6.98和2.90 nmol·L-1,等浓度配比下其联合毒性的EC50值分别为2.33、0.71和0.39 nmol·L-1,用相加指数判断其联合作用类型,在24 h显示为协同作用,其它两个时间段显示为拮抗效应,但从数值上可以发现协同和拮抗效应均不强,接近相加的联合效应类型.析因实验结果显示24、48和72 h这3个染毒时间段均显示了相加作用类型.析因实验与等浓度配比下获得的联合毒作用类型基本一致,但析因实验设计更为简捷方便,同时避免了由于计算EC50值产生的偏差对联合效应类型的影响.
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| 关 键 词： | 联合毒性 己烯雌酚 戊酸雌二醇 等浓度配比 析因实验设计 |
| 收稿时间： | 2012/10/17 0:00:00 |
| 修稿时间： | 2012/11/26 0:00:00 |
Study of Combined Effects of DES and EV on the Proliferation of MCF-7 Cells by Two Experimental Designs |
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| LIU Qian,LEI Bing-li,AN Jing,SHANG Yu,ZHONG Yu-fang,KANG Jia and WEN Yu.Study of Combined Effects of DES and EV on the Proliferation of MCF-7 Cells by Two Experimental Designs[J].Chinese Journal of Environmental Science,2013,34(8):3303-3308. |
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| Authors: | LIU Qian LEI Bing-li AN Jing SHANG Yu ZHONG Yu-fang KANG Jia and WEN Yu |
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| Institution: | Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China;Institute of Environmental Pollution and Health, College of Environmental and Chemical Engineering, Shanghai University, Shanghai 200444, China |
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| Abstract: | |
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| Keywords: | joint toxicity diethylstilbestrol β-estradiol 17-valerate equiconcentration ratio factorial experimental design |
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