Using lysosomal membrane stability of haemocytes in Ruditapes philippinarum as a biomarker of cellular stress to assess contamination by caffeine, ibuprofen, carbamazepine and novobiocin |
| |
Authors: | Gabriela V. Aguirre-Mart nez,Sara Buratti,Elena Fabbri,Angel T. DelValls M. Laura Mart n-D az |
| |
Affiliation: | Unitwin/Unesco/WiCoP, Physical Chemistry Department, University of Cádiz, Faculty of Marine and Environmental Sciences, Polígono Río San Pedro s/n, 11510 Puerto Real, Cádiz, Spain;Andalusian Center for Marine Science and Technology (CACYTMAR), Campus Universitario de Puerto Real, 11510, Puerto Real, Cádiz, Spain,University of Bologna, Interdepartment Centre for Research in Environmental Sciences (CIRSA), via S. Alberto 163, 48123 Ravenna, Italy,University of Bologna, Interdepartment Centre for Research in Environmental Sciences (CIRSA), via S. Alberto 163, 48123 Ravenna, Italy,Unitwin/Unesco/WiCoP, Physical Chemistry Department, University of Cádiz, Faculty of Marine and Environmental Sciences, Polígono Río San Pedro s/n, 11510 Puerto Real, Cádiz, Spain and Unitwin/Unesco/WiCoP, Physical Chemistry Department, University of Cádiz, Faculty of Marine and Environmental Sciences, Polígono Río San Pedro s/n, 11510 Puerto Real, Cádiz, Spain;Andalusian Center for Marine Science and Technology (CACYTMAR), Campus Universitario de Puerto Real, 11510, Puerto Real, Cádiz, Spain |
| |
Abstract: | Although pharmaceuticals have been detected in the environment only in the range from ng/L to μg/L, it has been demonstrated that they can adversely affect the health status of aquatic organisms. Lysosomal membrane stability (LMS) has previously been applied as an indicator of cellular well-being to determine health status in bivalve mussels. The objective of this study is to evaluate LMS in Ruditapes philippinarum haemolymph using the neutral red retention assay (NRRA). Clams were exposed in laboratory conditions to caffeine (0.1, 5, 15, 50 μg/L), ibuprofen (0.1, 5, 10, 50 μg/L), carbamazepine and novobiocin (both at 0.1, 1, 10, 50 μg/L) for 35 days. Results show a dose-dependent effect of the pharmaceuticals. The neutral red retention time measured at the end of the bioassay was significantly reduced by 50% after exposure to environmental concentrations (p < 0.05) (caffeine = 15 μg/L; ibuprofen = 10 μg/L; carbamazepine = 1 μg/L and novobiocin = 1 μg/L), compared to controls. Clams exposed to these pharmaceuticals were considered to present a diminished health status (retention time < 45 min), significantly worse than controls (96 min) (p < 0.05). The predicted no environmental effect concentration (PNEC) results showed that these pharmaceuticals are very toxic at the environmental concentrations tested. Measurement of the alteration of LMS has been found to be a sensitive technique that enables evaluation of the health status of clams after exposure to pharmaceuticals under laboratory conditions, thus representing a robust Tier-1 screening biomarker. |
| |
Keywords: | neutral red pharmaceuticals bioassay haemolymph bivalves health status Manila clam |
本文献已被 维普 万方数据 ScienceDirect 等数据库收录! |
| 点击此处可从《环境科学学报(英文版)》浏览原始摘要信息 |
|
点击此处可从《环境科学学报(英文版)》下载全文 |
|