Optimizing aerobic biodegradation of dichloromethane using
response surface methodology |
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Authors: | WU Shijin YU Xiang HU Zhihang ZHANG Lili and CHEN Jianmeng |
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Institution: | College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China |
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Abstract: | Response surface methodology (RSM) was employed to evaluate the optimum aerobic biodegradation of dichloromethane (DCM)
in pure culture. The parameters investigated include the initial DCM concentration, glucose as an inducer and hydrogen peroxide
as terminal electron acceptor (TEA). Maximum aerobic biodegradation efficiency was predicted to occur when the initial DCM
concentration was 380 mg/L, glucose 13.72 mg/L, and H2O2 115 mg/L. Under these conditions the aerobic biodegradation rate reached
up to 93.18%, which was significantly higher than that obtained under original conditions. Without addition of glucose, degradation
efficiencies were 6 80% at DCM concentrations < 326 mg/L. When concentrations of DCM were more than 480 mg/L, the addition of
hydrogen peroxide did not help to significantly increase DCM degradation efficiency. When DCM concentrations increased from 240
to 480 mg/L, the overall DCM degradation efficiency decreased from 91% to 60% in the presence of H2O2 for 120 mg/L. |
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Keywords: | biodegradation dichloromethane response surface methodology |
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