Toxicity screening of biodegradable polymers. I. Selection and evaluation of cell culture test methods |
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Authors: | M -H Dang F Birchler K Ruffieux E Wintermantel |
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Institution: | (1) ETH Zurich, Wagistr, 23, CH-8952 Schlieren, Switzerland |
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Abstract: | A screening test method for potential toxicity of biodegradable plastics on humans and the environment was selected and evaluated with samples of cellulose acetate, Bionolle, polyhydroxybutyrate-co-valerate (Biopol), and polycaprolactone (Tone polymer). Among the standardin vitro tests using animal cell cultures for the evaluation of biomedical materials, the test by direct contact and the test with extract were examined. Qualitative and quantitative determinations of the cell viability and morphology indicate that the test with extracts can be easily performed, providing reproducible and comparable results for all materials. Using the cell culture test with the extract of sterile samples, an estimation of the toxicity of a new polymeric material can be obtained within a few weeks. |
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Keywords: | Toxicity screening biodegradable polymers cell cultures test with extract |
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