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一株Pseudomonas sp.的分离鉴定与所产絮凝剂性能研究
引用本文:闫永胜,刘彬彬,毛艳丽,许化溪,苏兆亮.一株Pseudomonas sp.的分离鉴定与所产絮凝剂性能研究[J].环境科学与技术,2008,31(6).
作者姓名:闫永胜  刘彬彬  毛艳丽  许化溪  苏兆亮
作者单位:1. 江苏大学化学化工学院
2. 江苏大学环境学院
3. 江苏大学医学技术学院,江苏镇江,212013
基金项目:江苏大学校科研和教改项目
摘    要:从土壤中分离筛选出一株絮凝剂产生菌,命名为C-2,通过nPCR扩增试剂盒鉴定得出这株絮凝剂产生菌为荧光假单胞菌(Pseudomonas fluorescens);絮凝性能实验表明:生物絮凝剂(命名为PS-2)在碱性条件下,以CaCl2作为助凝剂处理高岭土悬浊液时,用量仅为20mL/L,搅拌强度要求不高,能够使高岭土的絮凝率达到98%以上,絮体形成快,矾花大,沉降速度快,且温度的影响不大;将PS-2应用于多种高浊度废水的处理中,结果表明其对多种废水均具有良好的絮凝效果;成分分析表明PS-2是一种多糖和核酸的混合物。

关 键 词:微生物絮凝剂  菌种鉴定  絮凝性能  成分分析

Isolation and Identification of Flocculant-producing Pseudomonas sp. and Study on Properties of Flocculant
YAN Yong-sheng,LIU Bin-bin,MAO Yan-li,XU Hua-xi,SU Zhao-liang.Isolation and Identification of Flocculant-producing Pseudomonas sp. and Study on Properties of Flocculant[J].Environmental Science and Technology,2008,31(6).
Authors:YAN Yong-sheng  LIU Bin-bin  MAO Yan-li  XU Hua-xi  SU Zhao-liang
Abstract:A bioflocculant-producing bacterium named C-2 was isolated from soil.The strain was identified as Pseudomonas fluorescens by nPCR amplification kit,and flocculating experiment showed that in alkali condition and at the presence of CaCl2,the bioflocculant named PS-2 had high efficiency with small dosage for dealing with kaolin suspension with larger floc,rapid sedimentation rate over a wide range of temperature.The bioflocculant had good flocculation activity to many kinds of wastewater.Component analysis showed that PS-2 was composed of polysaccharide and nucleic acid.
Keywords:bioflocculant  bacteria identification  flocculation property  component analysis
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