气态甲醛致雌性小鼠生殖细胞DNA-蛋白质交联的研究

为了研究气态甲醛对雌性小鼠生殖细胞的影响,以昆明雌性小鼠卵巢为实验材料,采取动态吸入式染毒方式,应用KCl-SDS沉淀法检测了气态甲醛染毒后所引起的小鼠卵巢生殖细胞DNA-蛋白质交联(DPC)效应.结果表明,较低浓度的甲醛(0.5mg·m-3),即可导致明显的DPC效应(与对照相比,p<0.05),并且随着染毒浓度的升高(0.5、1.0、3.0mg·m-3),DPC系数也逐渐升高.上述结果表明在实验所设浓度范围内,甲醛对小鼠卵巢生殖细胞的DNA损伤可以引起DNA-蛋白质的交联,并且DPC系数随着染毒浓度的增大而增大.DNA-蛋白质交联是DNA分子的一种严重损伤,气态甲醛对雌性小鼠生殖细胞具有一定的影响.

Study on DNA-Protein Crosslinks of Reproductive Cell of Female Mice Induced by Formaldehyde

In order to study the influence of gaseous formaldehyde(FA)on the reproductive cell of female mice, ovaries of Kunming female mice were used as experimental materials in the present study. Mice were exposed to gaseous FA, and KCl-SDS method was used to determine DNA-protein crosslinks(DPC)in mice ovaries. The results showed that FA at low concentration(0.5mg·m-3)could induce significant DPC formation(compared with the control group, p < 0.05),and DPC was increased with the increasing of FA concentrations(0.5, 1.0, 3.0mg·m-3). The result suggested that among the concentrations investigated in the present study, the form of DNA damage induced by gaseous FA on reproductive cell of female mice was DPC, and DPC was increased with the increasing of FA concentrations, indicating that gaseous FA has obvious genetic toxicity on the reproductive cells of female mice since DPC is a grievous damage of DNA.