| 具菲降解特性植物内生细菌的分离筛选及其生物学特性 |
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| 引用本文： | 陈小兵,盛下放,何琳燕,江春玉,孙乐妮,马海燕.具菲降解特性植物内生细菌的分离筛选及其生物学特性[J].环境科学学报,2008,28(7):1308-1313. |
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| 作者姓名： | 陈小兵 盛下放 何琳燕 江春玉 孙乐妮 马海燕 |
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| 作者单位： | 南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,南京,210095 |
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| 基金项目： | 国家自然科学基金
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高等学校学科创新引智计划(111计划) |
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| 摘 要： | 从长期受石油污染的植物体内分离到内生细菌36株,通过平皿促生和定殖试验筛选出1株能高效降解菲并明显促进小麦生长、能在小麦体内良好定殖的内生细菌7J2菌株.同时,对菌株7J2的生物学特性进行了研究.结果表明,菌株7J2在菲含量为30 mg·L-1的条件下,28℃振荡培养6d,菲的降解率达到99.81%.菌株7J2能够产生吲哚乙酸(IAA)(3.4±0.2)mg·L-1)和铁载体(2,3-二羟基苯甲酸)(4.3±1.6) mg·L-1).振荡培养3d,接活菌的培养基中有效磷浓度比接灭活菌的处理增加了32.5%.另外,菌株7J2对重金属Pb2 、Cr2 、Cu2 、Ni2 、Zn2 、Cd2 均有较强的抗性.通过16SrDNA序列测定,菌株7J2属于肠杆菌属.
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| 关 键 词： | 内生细菌 分离 菲降解 特性 |
| 收稿时间： | 5/25/2007 7:41:07 PM |
| 修稿时间： | 2007/9/28 0:00:00 |
Isolation and characteristics of a plant growth-promoting, phenanthrene-degrading endophytic bacterium from plants |
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| CHEN Xiaobing,SHENG Xiafang,HE Linyan,JIANG Chunyu,SUN leni and MA Haiyan.Isolation and characteristics of a plant growth-promoting, phenanthrene-degrading endophytic bacterium from plants[J].Acta Scientiae Circumstantiae,2008,28(7):1308-1313. |
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| Authors: | CHEN Xiaobing SHENG Xiafang HE Linyan JIANG Chunyu SUN leni and MA Haiyan |
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| Institution: | Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095,Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095 and Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095 |
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| Abstract: | |
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| Keywords: | endophytic bacterium isolation phenanthrene degradation characterization |
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