磺胺类制药废水中苯系物的快速同步检测

磺胺类制药废水是一类难降解有机废水，其中含有苯磺酰胺、苯磺酸、苯酚等苯系物，对这些物质的快速检测十分必要，针对这3种物质建立了基于高效液相色谱的快速同步检测方法。采用C₁₈柱为分离柱，比较了乙腈/水、甲醇/水和甲醇/缓冲盐3种流动相体系的分离效果，优化了230、240、250和260 nm 4种检测波长等条件。结果表明，V_甲醇∶V_{磷酸二氢铵溶液}(0.5%，pH=3.5)=50∶50作为流动相时，基线稳定，峰形较好，3种目标物在11 min内即可实现有效分离，且浓度为5~100 mg·L⁻¹时，3种目标物峰面积与质量浓度的线性关系良好(R²>0.999)，检出限为15.0~29.4 μg·L⁻¹，相对偏差为0.05%~1.56%(n=5)，该方法能同时检测苯磺酸、苯酚和苯磺酰胺，具有简便、灵敏、准确等优点，可为制药废水的快速检测和磺胺类药物降解机理的分析提供便利。

Rapid synchronous detection of benzene series in sulfonamide pharmaceutical wastewater

Sulfonamide pharmaceutical wastewater is a kind of refractory organic wastewater, which usually contains benzene series compounds e.g. benzenesulfonic acid, phenol and benzene. It is necessary to develop their fast detection methods. In this study, a rapid method for synchronous detection of benzenesulfonic acid, phenol and benzene sulfonamide in pharmaceutical wastewater was established based on high performance liquid chromatography. The reverse-phase C18 column was used, the separation effects of three mobile phase systems of acetonitrile/water, methanol/water and methanol/buffering salt were compared, and the four detection wavelengths of 230, 240, 250 and 260 nm was optimized. The results showed that the stable baseline and high-quality peak shape could be detected when Vmethanol∶Vammonium dihydrogen phosphate solution (0.5%, pH=3.5) = 50∶50 was used as the mobile phase, and the three target compounds could be well-separated within 11 min. Within the concentration range of 5~100 mg·L−1, good linear relationship between the peak area of the three targets and the mass concentration was achieved (R2>0.999). The detection limit and relative deviation were between 15.0~29.4 μg·L−1 and between 0.05%~1.56% (n=5), respectively. This method can effectively separate the three substances, and has the advantages of simplicity, sensitivity and accuracy. It can facilitate the fast detection of pharmaceutical wastewater and the analysis of the sulfonamides degradation mechanism.