反硝化生物滤池中生物膜量与脱氮效果和脱氢酶活性的关系

采用自主研发的中试反硝化生物滤池处理传统活性污泥法的二沉池出水，研究了稳定运行下生物膜量与脱氮效果和脱氢酶活性之间的关系。结果表明：根据VSS/SS=0.78、VSS/SS>0.78、VSS/SS<0.78，将SS分为3个区域，分别为区域1(232.5~1 246.6 mg·L−1)、区域2(1 246.6~2 542.7 mg·L−1)、区域3(2 542.7~3 523.9 mg·L−1)。在区域2内能获得最大的${rm{NO}}_3^ - $-N和TN去除能力，去除率分别为95.0%和85.7%及最大的总脱氢酶活性(TDHA)，为112.5 g；单位质量生物膜脱氢酶活性(DHA)与SS和VSS之间显著负相关，R2分别为0.822和0.876；TDHA随SS的增加而增加，直至VSS/SS开始减小时随之减小。DHA能较好地从微观层面反应微生物的活性，TDHA可从宏观层面反映整个反应器的生物活性，为反硝化生物滤池运行提供参考。

Relationship between biofilm mass and nitrogen removal efficiency,dehydrogenase activity in the denitrification biological filter

A self-developed pilot denitrification biological filter was used to study the relationship between biofilm mass and nitrogen removal efficiency, dehydrogenase activity in stable state when treating the secondary effluent from a conventional activated sludge process. The results showed that SS could be divided into three regions: region 1(232.5~1 246.6 mg·L−1), region 2(1 246.6~2 542.7 mg·L−1), region 3(2 542.7~3 523.9 mg·L−1) according to VSS/SS ratio equal to 0.78, higher than 0.78 or less than 0.78. In Region 2, the maximum removal rates of ${rm{NO}}_3^ - $-N and TN were obtained, and they were 95.0% and 85.7%, respectively. At the same time, the maximum total dehydrogenase activity(TDHA) was also obtained with a value of 112.5 g. Biological membrane dehydrogenase activity per unit mass (DHA) was negatively correlated with SS and VSS, with the R2 of 0.822 and 0.876, respectively. TDHA increased with the increase of SS, and decreased until VSS/SS began to decrease. DHA could better reflect the microbial activity at a micro-level, while TDHA could reflect the biological activity of the whole reactor at a macro-level, which provides theoretical basis for the denitrification biological filter operation.