高密度微阵列基因芯片技术在微生物分子生态学研究中的运用

应用基于16S rDNA 的高密度微阵列基因芯片(Microarray)技术对膜生物反应器内的微生物多样性进行研究.结果表明,膜生物反应器具有较高的微生物多样性,Microarray共检测到1 019种微生物.Proteobacteria是其中的优势种,共含有657种微生物,占总种群数量的64.5%左右.gamma Proteobacteria是Proteobacteria中的优势种,占35.8%左右,但相对含量一般并不高.beta Proteobacteria虽然种群数量稍少,但其在荧光强度最高的前25种和50种微生物中比重最大,分别占40%和36%左右.通过比较微生物的相对荧光强度,发现Clostridia是系统中的优势微生物种属.一些常见的硝化细菌如Nitrosomonadaceae、Nitrospiraceae等也具有较高的含量.Microarray作为一种实时、高效、准确的分子生物学手段,可应用于废水处理中的微生物多样性研究.

Using High-density Universal 16S rRNA Microarray in Microbial Molecular Ecology Research

High-density universal 16S rRNA Microarray was used to detect the microbial community in membrane bioreactor (MBR). The results showed MBR had high microbial diversity. 1 019 bacteria were detected by Miroarray in MBR. Proteobacteria was the predominant phylum. It had 657 OTUs, occupying 64.5% of the total bacteria. gamma Proteobacteria was the main class of Proteobacteria, which occupied 35.8% of Proteobacteria, but the average fluorescence intensity of was not very high. Despite the bacteria diversity of beta Proteobacteria was lower than gamma Proteobacteria. However, it occupied 40% and 36% in the 25 and 50 dominant bacteria according to relative average fluorescence intensity. Clostridia had a relative high concentration when compare the average fluorescence intensity of the whole bacteria in MBR. Some common nitrify bacteria such as Nitrosomonadaceae and Nitrospiraceae were also detected and had high fluorescence intensity in the system. Microarray is a newer molecular method and has some advantages such as real time, high efficiency and exact results. It's very suitable for investigation of microbial diversity in wastewater treatment.