可降解壬基酚的产电假单胞菌LQF-6的分离及其特性研究

从稳定运行以壬基酚为燃料的微生物燃料电池阳极生物膜分离到一株能以壬基酚为唯一碳源生长的产电菌LQF-6。对菌株LQF-6的形态和16S rDNA序列分析表明该菌为假单胞菌。LQF-6以壬基酚为唯一碳源,培养84 h壬基酚降解率为53.80%。差分脉冲伏安测定结果表明,LQF-6分别在-0.088 V和0.272 V出现两个氧化峰。菌株LQF-6接种至微生物燃料电池阳极室,以壬基酚为底物,铁氰化钾为阴极电子受体,外接电阻为1000Ω时,微生物燃料电池最大输出电压达0.17 V,电池最大功率密度为(58.51±2.24)m W/m²。

Isolation of a nonylphenol degradation electrogen Psedudomonas LQF-6 and analysis of its special electrochemical feature

The given paper is to try to isolate a group of nonylphenol degradation electrogenic bacteria(short for LQF-6) from the biofilm attached to the anode of the double chamber microbial fuel cell(MFC) by taking nonylphenol as the fuel for 18 months via the stable operation of the plate spreading method. And,then,the isolated electrogenic bacteria known as LQF-6 can be identified with the help of the electronic microscope morphological observation and 16 S rDNA sequence analysis. And,next,we have also made efforts to examine the electrochemical characterization and nonylphenol degradation performance of the strain LQF-6. And,fruitfully,the electronic scanning microscope image may demonstrate that the isolated strain LQF-6 tends to be a rod-shaped bacteria,ranging from 0. 6 μm to 1. 5 μm in length and about 0. 4 μm in width. The 16 S rDNA sequence of LQF-6 can be submitted to the Gen Bank and assigned to be the sequence number of MN736490. And,afterwards,the phylogenetic tree analysis of the 16 S rDNA sequence may tell us that the strain LQF-6 and Psedudomonas delhiensis RLD-1(NR043731)can remain to be clustered in the same branch. And,then,combined with the morphological features of the strain,it can further help us to confirm that the isolated strain LQF-6 belongs to the genus Pseudomonas. The above results may also suggest that the degradation rate of the nonylphenol ought to be positively related to the time of its treatment. To be accurate,the nonylphenol concentration tends to decrease from the initial 20 mg/L to( 9. 24 ± 0. 86) mg/L through a treating time of 84 h. Nevertheless,the concentration rate of nonylphenol can only be made decreased by 0. 44 mg/L through a 96 h treating time to be extended from 84 h. And,according to the differential pulse voltammetry analysis of the strain LQF-6,there ought to be 2 oxidation peaks in a length from-0. 088 V to 0. 272 V. And,when the strain LQF-6 is inoculated into the anode chamber of MFC with nonylphenol as fuel,as well as the potassium ferricyanide as the electron acceptor in the cathode chamber,the output voltage of the MFC can be made to reach 0. 17 V through a running process of 20 hours. And,consequently,the output voltage can be supposed to decrease bit by bit in consumption of nonylphenol in the anolyte. On the other hand,the polarization analysis also demonstrates that the maximum power density generated by the strain LQF-6 in MFC with nonylphenol as fuel can be expected to reach(58. 51 ± 2. 24) m W/m2 with an external resistance load of 1000 Ω.