Assessing the bioavailability of phenanthrene to soil microorganisms using the Tenax extraction method |
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Authors: | Bozena Smreczak Barbara Maliszewska-Kordybach Agnieszka Klimkowicz-Pawlas |
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Institution: | (1) Institute of Soil Science and Plant Cultivation – National Research Institute, ul. Czartoryskich 8, 24-100 Pulawy, Poland |
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Abstract: | The Tenax TA extraction technique followed by gas chromatography mass spectrometry (GC-MS) determinations was used to assess
the actually bioavailable fraction of phenanthrene (ABF-Phe) in three different soils freshly contaminated with this compound
at levels of 10, 100, and 1,000 mg kg−1. The results were related to the ecotoxic effect of phenanthrene on soil nitrifying bacteria. Nitrification potential measurements
were applied as an ecotoxicity end point. A strong positive linear relationship (r
2 = 0.95) was obtained between the content of the actually bioavailable phenanthrene fraction and the inhibition of nitrifying
bacteria activity. |
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Keywords: | Bioavailability Phenanthrene Potential nitrification Tenax TA |
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